Dynamics of the Pulpo-Dentin Complex

Pashley, David H.

doi:10.1177/10454411960070020101

Cited by 376 publications

(317 citation statements)

References 155 publications

(208 reference statements)

Supporting

Mentioning

255

Contrasting

Unclassified

Order By: Relevance

“…32 In spite of these chemical differences between dentin and pulp, the two are functionally coupled and hence are integrated as a tissue. 33 When normal teeth are subjected to any type of insult or trauma the pulp responds to these insults, for a short-term by an inflammatory response, and in the long-term by inducing the cytodifferentiation of odontoblast-like cells to produce reparative dentin as a biologic response to reduce the permeability of the pulpdentin complex and to restore it to its original sequestration. 33 In order to generate normal dentin-like tissue, the first requirement would be to mimic the physiologic environment.…”

Section: Dentin Matrix Protein 1 Induces Cytodifferentiation a Almushmentioning

confidence: 99%

“…33 When normal teeth are subjected to any type of insult or trauma the pulp responds to these insults, for a short-term by an inflammatory response, and in the long-term by inducing the cytodifferentiation of odontoblast-like cells to produce reparative dentin as a biologic response to reduce the permeability of the pulpdentin complex and to restore it to its original sequestration. 33 In order to generate normal dentin-like tissue, the first requirement would be to mimic the physiologic environment. For this, there should be cells that are competent and able to incorporate signals that are responsible for their cytodifferentiation to odontoblastlike cells, next there should be suitable induction signal(s) that will be able to induce these competent cells.…”

Section: Dentin Matrix Protein 1 Induces Cytodifferentiation a Almushmentioning

confidence: 99%

See 1 more Smart Citation

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

et al. 2005

View full text Add to dashboard Cite

Odontoblasts are postmitotic cells that differentiate from the dental papilla. These cells are responsible for producing the calcified dentin matrix. The pulp-odontoblast interphase contains undifferentiated mesenchymal stem cells, which have the ability to cytodifferentiate into odontoblast-like cells in response to specific signaling molecules. Dentin matrix protein 1 (DMP1) is one of the dentin noncollagenous extracellular matrix proteins that has been implicated in regulation of mineralization. In this study, we have examined the potential role of DMP1 in inducing cytodifferentiation of dental pulp stem cells into odontoblast-like cells and formation of reparative dentin in a rat model. Cavities were drilled and pulps exposed in maxillary first molars. Collagen matrix impregnated with recombinant DMP1 was implanted directly in Group 1, while calcium hydroxide, a commonly used pulp-capping agent was implanted in group 2, collagen matrix that was not impregnated with rDMP1 was implanted directly in group 3, which served as control. Each of these three groups was subdivided into two subgroups, A for 2 weeks time duration and B for 4 weeks duration. At the end of the time period the maxillae were excised, tissues were processed for histological and immunohistochemical evaluations. The results showed that DMP1 could act as a morphogen on undifferentiated mesenchymal cells present in the dentin-pulp complex. These differentiated cells had the potential to regenerate dentin-like tissue, which was confirmed by the presence of collagenous matrix, odontoblast specific markers and calcified deposits. Gene Therapy (2006) 13, 611-620.

show abstract

Section: Dentin Matrix Protein 1 Induces Cytodifferentiation a Almushmentioning

confidence: 99%

Section: Dentin Matrix Protein 1 Induces Cytodifferentiation a Almushmentioning

confidence: 99%

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

et al. 2005

View full text Add to dashboard Cite

show abstract

“…35 The shear strength of dentin differs in the central and cusp areas and is dependent on dentinal tubule orientation in the central area. 36 However, tubule orientation has no appreciable effect on the elastic behavior of normal dentin, as intertubular dentin governs the elastic behavior of the dentin substrate.…”

mentioning

confidence: 99%

Bonding Efficacy of Single-step Self-etch Systems to Sound Primary and Permanent Tooth Dentin

Uekusa

Yamaguchi

Miyazaki

et al. 2006

Operative Dentistry

View full text Add to dashboard Cite

Single-step self-etch systems are capable of producing a predictable bond to primary dentin, although the bond strength was found to be lower than permanent dentin. SUMMARYCurrently, there is little information regarding the bonding efficacy of single-step self-etch systems to primary tooth dentin. This study examined the microtensile bond strength of single-step self-etch systems (Clearfil tri-S Bond and One-Up Bond F Plus) to sound primary and permanent tooth dentin. Adhesives were applied to flat samples of primary and permanent tooth dentin, and resin composites were bonded according to the manufacturers' instructions. After 24 hours of storage in distilled water at 37°C, hour glass-shaped specimens were produced. They were subjected to microtensile testing at a crosshead speed of 1.0 mm/minute. The results were analyzed using 2-way analysis of variance (ANOVA) followed by the Tukey HSD post-hoc test (α α=0.05). Field-emission scanning electron microscopy (FE-SEM) observations of the adhesive-treated dentin surfaces and the resin/dentin interface were also conducted. The bond strengths of primary tooth dentin were significantly lower than that of permanent tooth dentin for both self-etch systems: 44.7 ± 10.4 versus 54.3 ± 9.0 MPa for Clearfil tri-S Bond and 40.6 ± 9.9 versus 50.0 ± 8.7 MPa for One-Up Bond F Plus (p<0.001). There was no statistically significant interaction between the type of adhesive system and the dentin substrate (p=0.957). Although there was no statistically significant difference in the mean values among the different adhesive systems (p=0.094), there was a statistically significant difference in mean values among the different dentin substrates (p<0.001), which were lower for primary tooth dentin than for permanent tooth Operative Dentistry dentin. The failure modes were also independent of the type of dentin but dependent on the adhesive systems, an equal distribution among the 3 types of failure for Clearfil tri-S Bond and cohesive failures in adhesives for One-Up Bond F Plus. FE-SEM observations of dentin to which adhesive had been applied revealed that the smear layer had been removed and the collagen fibers exposed. Though the bond strengths to primary tooth dentin were lower than to permanent tooth dentin, excellent adaptation of the single-step selfetch systems to both dentin substrates was observed by FE-SEM. Further studies are required to determine the long-term clinical performance of these adhesive systems when applied to primary tooth dentin.

show abstract

“…However, long-term studies have shown that the rate of tooth loss is higher for endodontically treated teeth compared to nontreated teeth, due to fracture, secondary caries and complex restoration-associated problems [5,6,7,8]. This has been one of the central challenges in dentistry, and thus the improvement of antimicrobial agents and restorative materials and the diagnosis of pulp vitality to facilitate the control of infection are great interest [9]. The treatment of irreversible pulpitis aims to restore the original architecture and biological function of the infected pulp tissue by facilitating repair or by allowing for tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effects of Matrix Metalloproteinase-3 on Irreversible Pulpitis of Mature Erupted Teeth

Hira

Kobayashi²,

Kawashiri³

et al. 2014

Journal of Oral and Maxillofacial Surgery

View full text Add to dashboard Cite

Matrix metalloproteinases (MMPs) are involved in extracellular matrix degradation and the modulation of cell behavior. These proteinases have also been implicated in tissue repair and regeneration. Our previous studies have demonstrated that MMP-3 elicits stimulatory effects on the proliferation and the migration of endothelial cells as well as anti-apoptotic effects on these cells in vitro. In addition, we found that MMP-3 enhanced the regeneration of lost pulp tissue in a rat incisor pulp injury model. However, continuously erupting rodent incisors exhibit significantly different pulp organization compared with mature erupted teeth. Therefore, we have further extended these studies using a canine irreversible pulpitis model to investigate the effects of MMP-3. In this study, the crowns of the canine mature premolars were removed and the pulp tissues were amputated. The amputated pulp tissues remained exposed for 24 or 72 hours to induce mild or severe irreversible pulpitis, respectively, followed by sealing of the cavities. In both models, the whole pulp tissues became necrotic by day 14. In this mild pulpitis model, the regeneration of pulp tissue with vasculature and nerves was observed until 14 days after sealing with MMP-3, followed by extracellular matrix formation in the regenerated pulp tissues until day 28. The treatment with MMP-3 resulted in a decrease in the number of macrophage and antigen-presenting cells and a significant inhibition of IL-6 expression on day 3. The inhibition of MMP-3 activity abolished these anti-inflammatory effects. Immunofluorescence staining demonstrated that MMP-3 was involved in the modification of serum-derived hyaluronanassociated proteins and hyaluronan (SHAP-HA) complexes possibly through the degradation of versican. These results demonstrate that MMP-3 can act as an anti-inflammatory agent and suggest that MMP-3 might represent a useful therapy for the treatment of mild irreversible pulpitis.

show abstract

Dynamics of the Pulpo-Dentin Complex

Cited by 376 publications

References 155 publications

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

Dentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblasts

Bonding Efficacy of Single-step Self-etch Systems to Sound Primary and Permanent Tooth Dentin

Anti-Inflammatory Effects of Matrix Metalloproteinase-3 on Irreversible Pulpitis of Mature Erupted Teeth

Contact Info

Product

Resources

About