2018
DOI: 10.1128/jvi.01418-18
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Dynamin Is Required for Efficient Cytomegalovirus Maturation and Envelopment

Abstract: Cytomegalovirus secondary envelopment occurs in a virus-induced cytoplasmic assembly compartment (vAC) generated via a drastic reorganization of the membranes of the secretory and endocytic systems. Dynamin is a eukaryotic GTPase that is implicated in membrane remodeling and endocytic membrane fission events; however, the role of dynamin in cellular trafficking of viruses beyond virus entry is only partially understood. Mouse embryonic fibroblasts (MEF) engineered to excise all three isoforms of dynamin were i… Show more

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Cited by 11 publications
(13 citation statements)
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“…3A, insets). Perinuclear clathrin accumulation in infected fibroblasts is consistent with previous findings that clathrin is re-localized to the VAC during infection 40,41 . However, the re-localization of clathrin to the VAC in infected HMVECs is less prominent.…”
Section: Clathrin Heavy Chain Associates With Ul32-gfp-positive Vesicles Clathrin Acts Assupporting
confidence: 92%
“…3A, insets). Perinuclear clathrin accumulation in infected fibroblasts is consistent with previous findings that clathrin is re-localized to the VAC during infection 40,41 . However, the re-localization of clathrin to the VAC in infected HMVECs is less prominent.…”
Section: Clathrin Heavy Chain Associates With Ul32-gfp-positive Vesicles Clathrin Acts Assupporting
confidence: 92%
“…The dynasore treatment at 4 hpi abolished the expression of unprocessed M55 protein (130 kDa) and the cleaved M55 fragment (55 kDa) ( Figure 9 C). These data are consistent with the observation of inhibited MCMV late protein (M55/gB) expression in triple dynamin knock-out cells [ 38 ]. Interestingly, the same result was achieved when cells were incubated with dynasore only during the period of 4–14 h after infection.…”
Section: Resultssupporting
confidence: 92%
“…The role of dynamin in CMV assembly and egress has been addressed in two recent studies. In the HCMV study [ 48 ], the dynamin role was also assessed using dynasore, whereas in the MCMV study [ 38 ] by monitoring virion production in the triple dynamin knock-out cells. Both studies agreed with the essential role of dynamin in virion production and suggested that its activity may be related to the establishment of the AC.…”
Section: Discussionmentioning
confidence: 99%
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