Dynamin Participates in the Maintenance of Anterior Polarity in the Caenorhabditis elegans Embryo

Nakayama, Yuji; Shivas, Jessica M.; Poole, Daniel S.; Squirrell, Jayne M.; Kulkoski, Jennifer M.; Schleede, Justin; Skop, Ahna R.

doi:10.1016/j.devcel.2009.04.009

Cited by 48 publications

(65 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, our FRAP analysis indicates that GPB-1 is internalized at comparable rates across the cell cycle (Fig. 3D), whereas the rate of endocytosis varies across the cell cycle in the early embryo (Nakayama et al, 2009). Second, we found that levels of GPB-1 at the cell membrane do not increase upon 2779 RESEARCH ARTICLE Gb trafficking in C. elegans embryos Table S2 in the supplementary material for actual values and statistical analysis.…”

Section: Gb Trafficking In Early C Elegans Embryosmentioning

confidence: 74%

“…Therefore, we reasoned that short exposures of dyn-1(ky51) embryos to the restrictive temperature followed by immunofluorescence analysis should provide a snapshot of the extent of GPB-1 trafficking at each stage of the cell cycle (see Materials and methods). In addition, we reasoned that such shortterm inactivation of dynamin function should not alter A-P polarity, which is also dynamin dependent (Nakayama et al, 2009), thus simplifying the interpretation of these experiments. As shown in Fig.…”

Section: Gpb-1 Endosomal Association Is Maximal During Mitosismentioning

confidence: 99%

“…Importantly, we found that the asymmetry in GPB-1 trafficking is lost upon inactivation of A-P polarity cues. Interestingly, however, this asymmetry is maintained in dyn-1(RNAi) embryos, in which the maintenance but not the establishment phase of A-P polarity is affected (Nakayama et al, 2009). This suggests that the asymmetry of GPB-1 trafficking is under the control of A-P polarity specifically during the establishment phase.…”

Section: Polarity Asymmetric Trafficking and Gbg Regulationmentioning

confidence: 99%

“…The anterior PAR polarity components PAR-6/PKC-3 and CDC-42 regulate intracellular trafficking, including recycling of clathrin-independent cargo (Balklava et al, 2007). In fact, the asymmetric distribution of dynamin, early endosomes and recycling endosomes is under the control of anterior PAR proteins (Andrews and Ahringer, 2007;Balklava et al, 2007;Nakayama et al, 2009), and overall intracellular trafficking is enriched on the anterior side (Nakayama et al, 2009). However, whether such an asymmetry holds true for specific effector cargoes implicated in asymmetric spindle positioning was not known prior to our work.…”

Section: Polarity Asymmetric Trafficking and Gbg Regulationmentioning

confidence: 99%

See 3 more Smart Citations

Polarity mediates asymmetric trafficking of the Gβ heterotrimeric G-protein subunit GPB-1 inC. elegansembryos

2011

View full text Add to dashboard Cite

SUMMARYAsymmetric cell division is an evolutionarily conserved process that gives rise to daughter cells with different fates. In one-cell stage C. elegans embryos, this process is accompanied by asymmetric spindle positioning, which is regulated by anterior-posterior (A-P) polarity cues and driven by force generators located at the cell membrane. These force generators comprise two G proteins, the coiled-coil protein LIN-5 and the GoLoco protein GPR-1/2. The distribution of GPR-1/2 at the cell membrane is asymmetric during mitosis, with more protein present on the posterior side, an asymmetry that is thought to be crucial for asymmetric spindle positioning. The mechanisms by which the distribution of components such as GPR-1/2 is regulated in time and space are incompletely understood. Here, we report that the distribution of the Gb subunit GPB-1, a negative regulator of force generators, varies across the cell cycle, with levels at the cell membrane being lowest during mitosis. Furthermore, we uncover that GPB-1 trafficks through the endosomal network in a dynamin-and RAB-5-dependent manner, which is most apparent during mitosis. We find that GPB-1 trafficking is more pronounced on the anterior side and that this asymmetry is regulated by A-P polarity cues. In addition, we demonstrate that GPB-1 depletion results in the loss of GPR-1/2 asymmetry during mitosis. Overall, our results lead us to propose that modulation of Gb trafficking plays a crucial role during the asymmetric division of one-cell stage C. elegans embryos.

show abstract

Section: Gb Trafficking In Early C Elegans Embryosmentioning

confidence: 74%

Section: Gpb-1 Endosomal Association Is Maximal During Mitosismentioning

confidence: 99%

Section: Polarity Asymmetric Trafficking and Gbg Regulationmentioning

confidence: 99%

Section: Polarity Asymmetric Trafficking and Gbg Regulationmentioning

confidence: 99%

See 2 more Smart Citations

Polarity mediates asymmetric trafficking of the Gβ heterotrimeric G-protein subunit GPB-1 inC. elegansembryos

2011

View full text Add to dashboard Cite

show abstract

“…LGL-1 homologs have also been shown to regulate polarized vesicle trafficking (Brennwald and Rossi, 2007), and a recent report revealed a requirement for dynamin-mediated endocytosis in maintaining polarity in the zygote (Nakayama et al, 2009). However, dynamin is enriched in the anterior of the embryo, where increased levels of endocytosis are observed, and is therefore unlikely to function directly with LGL-1 to maintain polarity.…”

Section: Reciprocal Inhibitory Interactions Between Par Proteinsmentioning

confidence: 99%

Elaborating polarity: PAR proteins and the cytoskeleton

Nance¹,

2011

View full text Add to dashboard Cite

Cell polarity is essential for cells to divide asymmetrically, form spatially restricted subcellular structures and participate in three-dimensional multicellular organization. PAR proteins are conserved polarity regulators that function by generating cortical landmarks that establish dynamic asymmetries in the distribution of effector proteins. Here, we review recent findings on the role of PAR proteins in cell polarity in C. elegans and Drosophila, and emphasize the links that exist between PAR networks and cytoskeletal proteins that both regulate PAR protein localization and act as downstream effectors to elaborate polarity within the cell.

show abstract

Anterior PAR proteins function during cytokinesis and maintain DYN‐1 at the cleavage furrow in Caenorhabditis elegans

Pittman

Skop

2012

Cytoskeleton

Self Cite

View full text Add to dashboard Cite

PAR proteins are key regulators of cellular polarity and have links to the endocytic machinery and the actin cytoskeleton. Our data suggest a unique role for PAR proteins in cytokinesis. We have found that at the onset of cytokinesis, anterior PAR-6 and posterior PAR-2 proteins are redistributed to the furrow membrane in a temporal and spatial manner. PAR-6 and PAR-2 localize to the furrow membrane during ingression but PAR-2-GFP is distinct in that it is excluded from the extreme tip of the furrow. Once the midbody has formed, PAR-2-GFP becomes restricted to the midbody region (the midbody plus the membrane flanking it). Depletion of both anterior PAR proteins, PAR-3 and PAR-6, led to an increase in multinucleate embryos, suggesting that the anterior PAR proteins are necessary during cytokinesis and that PAR-3 and PAR-6 function in cytokinesis may be partially redundant. Lastly, anterior PAR proteins play a role in the maintenance of DYN-1 in the cleavage furrow. Our data indicate that the PAR proteins are involved in the events that occur during cytokinesis and may play a role in promoting the membrane trafficking and remodeling events that occur during this time.

show abstract

Dynamin Participates in the Maintenance of Anterior Polarity in the Caenorhabditis elegans Embryo

Cited by 48 publications

References 71 publications

Polarity mediates asymmetric trafficking of the Gβ heterotrimeric G-protein subunit GPB-1 inC. elegansembryos

Polarity mediates asymmetric trafficking of the Gβ heterotrimeric G-protein subunit GPB-1 inC. elegansembryos

Elaborating polarity: PAR proteins and the cytoskeleton

Anterior PAR proteins function during cytokinesis and maintain DYN‐1 at the cleavage furrow in Caenorhabditis elegans

Contact Info

Product

Resources

About