Dysbiosis of gut microbiome affecting small intestine morphology and immune balance: a rhesus macaque model

Li, Hong-Zhe; Li, Nan; Wang, Jingjing; Li, Heng; Huang, Xing; Guo, Lei; Zheng, Huiwen; He, Zhanlong; Zhao, Yuan; Yang, Zening; Fan, Haitao; Chu, Manman; Yang, Jun; Wu, Qiongwen; Liu, Long-ding

doi:10.24272/j.issn.2095-8137.2020.004

Cited by 13 publications

(6 citation statements)

References 40 publications

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“…Gut microbiota plays a crucial role in the health of animal hosts through many factors, such as genetics, geography, nutrition, diet, and immunity ( Kohl, 2012 ; van Dongen et al, 2013 ; Kong et al, 2014 ; Waite and Taylor, 2014 , 2015 ). It is well-established that the gut microbiota of an animal functions from the moment the animal is born, the amount and species of gut microbiota are not fixed ( Xiong et al, 2019 ; Li et al, 2020 ). The external environment and the host itself influence the diversity of gut microorganisms ( Ley et al, 2006 ; Mueller et al, 2006 ; Waite and Taylor, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Gut Microbiota in Captive and Wild Oriental White Storks: Implications for Conservation Biology

Zhou

et al. 2021

Front. Microbiol.

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The oriental white stork (Ciconia boyciana) is considered an endangered species based on the International Union for Conservation of Nature (IUCN) Red List. This study presents the first evidence on comparative analysis of gut microbial diversity of C. boyciana from various breeding conditions. To determine the species composition and community structure of the gut microbiota, 24 fecal samples from Tianjin Zoo and Tianjin Qilihai Wetland were characterized by sequencing 16S rRNA gene amplicons using the Illumina MiSeq platform. Firmicutes was found to be the predominant phylum. Analysis of community structure revealed significant differences in the species diversity and richness between the populations of the two breeding conditions. The greatest α-diversity was found in wild C. boyciana, while artificial breeding storks from Tianjin Zoo had the least α-diversity. Principal coordinates analysis showed that the microbial communities were different between the two studied groups. In conclusion, this study reveals the species composition and structure of the gut microbiota of oriental white storks under two breeding conditions, and our findings could contribute to the integrative conservation of this endangered bird.

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Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Gut Microbiota in Captive and Wild Oriental White Storks: Implications for Conservation Biology

Zhou

et al. 2021

Front. Microbiol.

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“…Soil available nitrogen (AN) was determined by diffusion absorption method ( Michrina et al, 1982 ). Soil available phosphorus (AP) was analyzed following the methods described by Li et al, (2020) . Soil available potassium (AK) was determined by atomic absorption spectrometry ( Tian et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…The V5-V7 region of fungal 18S rRNA was amplified by using universal primers SSU0817F (5’-TTAGCATGGAATAATRRAATAGGA-3′) and 1196R (5’-TCTGGACCTGGTGAGTTTCC-3′; Jiang et al, 2017 ). The V3-V4 region of 16S rRNA was PCR amplified by using universal primers 338\u00B0F (5’-ACTCCTACGGGAGGCAGCA-3′) and 806 R (5’-GGACTACHVGGGTWTCTAAT-3′; Li et al, 2020 ). Pfu high-fidelity DNA polymerase from TransGen Biotech was used for PCR amplification with the number of amplification cycles strictly controlled to make cycle number as small as possible and the amplification conditions for the same batch of samples consistent.…”

Section: Methodsmentioning

confidence: 99%

Coptischinensis Franch root rot infection disrupts microecological balance of rhizosphere soil and endophytic microbiomes

Tang

Wang

Fang³

et al. 2023

Front. Microbiol.

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IntroductionThe ecological balance of the plant microbiome, as a barrier against pathogens, is very important for host health. Coptis chinensis is one of the important medicinal plants in China. In recent years, Illumina Miseq high-throughput sequencing technology was frequently used to analyze root rot pathogens and the effects of root rot on rhizosphere microorganisms of C. chinensis. But the effects of root rot infection on rhizosphere microecological balance of C. chinensis have received little attention.MethodsIn this study, Illumina Miseq high-throughput sequencing technology was applied to analyze the impact on microbial composition and diversity of C. chinensis by root rot.ResultsThe results showed that root rot infection had significant impact on bacterial α-diversity in rhizome samples, but had no significant effect on that in leaf samples and rhizosphere soil samples, while root rot infection exhibited significant impact on the fungal α-diversity in leaf samples and rhizosphere soil samples, and no significant impact on that in rhizome samples. PCoA analysis showed that the root rot infection had a greater impact on the fungal community structure in the rhizosphere soil, rhizome, and leaf samples of C. chinensis than on the bacterial community structure. Root rot infection destroyed the microecological balance of the original microbiomes in the rhizosphere soil, rhizome, and leaf samples of C. chinensis, which may also be one of the reasons for the serious root rot of C. chinensis.DiscussionIn conclusion, our findings suggested that root rot infection with C. chinensis disrupts microecological balance of rhizosphere soil and endophytic microbiomes. The results of this study can provide theoretical basis for the prevention and control of C. chinensis root rot by microecological regulation.

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“…16 The composition of the gut microbiome, especially the abundance of commensal species, is crucial to regulating the pathogenesis of inflammatory conditions such as infection and inflammatory bowel disease. [17][18][19][20][21] Gut bacterial homeostasis is maintained by the mucus lining secreted by goblet cells, 13,22,23 the tight junction proteins that allow selective permeability of small molecules across the epithelial layer, 24,25 as well as AMPs produced by the specialized epithelial Paneth cells found in the intestinal crypt. 26,27 The potential cell-type-specific role of AHR in protecting the small intestine through activating signaling pathways that lead to AMP production is yet to be fully understood.…”

Section: Introductionmentioning

confidence: 99%

“…The epithelial barrier provides a protective surface, spatially separating the intestine from the bacteria in the lumen 16 . The composition of the gut microbiome, especially the abundance of commensal species, is crucial to regulating the pathogenesis of inflammatory conditions such as infection and inflammatory bowel disease 17–21 . Gut bacterial homeostasis is maintained by the mucus lining secreted by goblet cells, 13,22,23 the tight junction proteins that allow selective permeability of small molecules across the epithelial layer, 24,25 as well as AMPs produced by the specialized epithelial Paneth cells found in the intestinal crypt 26,27 .…”

Section: Introductionmentioning

confidence: 99%

Immune cell‐intrinsic Ah receptor facilitates the expression of antimicrobial REG3G in the small intestine

Chakraborty,

Coslo,

Murray

et al. 2024

The FASEB Journal

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The intestinal epithelial layer is susceptible to damage by chemical, physiological and mechanical stress. While it is essential to maintain the integrity of epithelium, the biochemical pathways that contribute to the barrier function have not been completely investigated. Here we demonstrate an aryl hydrocarbon receptor (AHR)‐dependent mechanism facilitating the production of the antimicrobial peptide AMP regenerating islet‐derived protein 3 gamma (REG3G), which is essential for intestinal homeostasis. Genetic ablation of AHR in mice impairs pSTAT3‐mediated REG3G expression and increases bacterial numbers of Segmented filamentous bacteria (SFB) and Akkermansia muciniphila in the small intestine. Studies with tissue‐specific conditional knockout mice revealed that the presence of AHR in the epithelial cells of the small intestine is not required for the production of REG3G through the phosphorylated STAT3‐mediated pathway. However, immune‐cell‐specific AHR activity is necessary for normal expression of REG3G in all regions of the small intestine. A diet rich in broccoli, capable of inducing AHR activity, increases REG3G production when compared to a semi‐purified diet that is devoid of ligands that can potentially activate the AHR, thus highlighting the importance of AHR in antimicrobial function. Overall, these data suggest that homeostatic antimicrobial REG3G production is increased by an AHR pathway intrinsic to the immune cells in the small intestine.

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Dysbiosis of gut microbiome affecting small intestine morphology and immune balance: a rhesus macaque model

Cited by 13 publications

References 40 publications

Comparative Analysis of Gut Microbiota in Captive and Wild Oriental White Storks: Implications for Conservation Biology

Comparative Analysis of Gut Microbiota in Captive and Wild Oriental White Storks: Implications for Conservation Biology

Coptischinensis Franch root rot infection disrupts microecological balance of rhizosphere soil and endophytic microbiomes

Immune cell‐intrinsic Ah receptor facilitates the expression of antimicrobial REG3G in the small intestine

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