Dysbiosis of the Human Urinary Microbiome and its Association to Diseases Affecting the Urinary System

Jayalath, Shehani; Magana-Arachchi, Dhammika

doi:10.1007/s12088-021-00991-x

Cited by 14 publications

(4 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Apart from impaired goblet cell function, structural disruption of the gut microbiota is an etiological factor in enteric diseases [78]. The 16S rDNA approach has been widely applied to investigate microbial alternations in pathogen-infective diseases [79,80]. After early weaning, the gut microbiome is not yet well-established and not yet mature under the invasion of pathogenic bacteria [81].…”

Section: Discussionmentioning

confidence: 99%

Effects of Enterotoxigenic Escherichia coli Challenge on Jejunal Morphology and Microbial Community Profiles in Weaned Crossbred Piglets

Xu,

Jia,

Xiao

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Pathogenic enterotoxigenic Escherichia coli (ETEC) is a major cause of bacterial diarrhea in weaning piglets, which are vulnerable to changes in environment and feed. This study aimed to determine the effects of the ETEC challenge on piglet growth performance, diarrhea rate, jejunal microbial profile, jejunal morphology and goblet cell distribution. A total of 13 piglets from one litter were selected on postnatal day 21 and assigned to treatments with or without ETEC challenge at 1 × 108 CFUs, as ETEC group or control group, respectively. On postnatal day 28, samples were collected, followed by the detection of serum biochemical indexes and inflammatory indicators, HE staining, PAS staining and 16S rDNA gene amplicon sequencing. Results showed that the growth performance decreased, while the diarrhea rate increased for the ETEC group. The jejunum is the main segment of the injured intestine during the ETEC challenge. Compared with the control, the ETEC group displayed fewer goblet cells in the jejunum, where goblet cells are more distributed at the crypt and less distributed at the villus. In addition, ETEC piglets possessed higher abundances of the genus Desulfovibrio, genus Oxalobacter and genus Peptococus and lower abundances of the genus Prevotella 2, genus Flavonifractor and genus Blautra. In terms of alpha diversity, Chao 1 and observed features indexes were both increased for the ETEC group. Our study provides insights into jejunal histopathological impairment and microbial variation in response to ETEC infection for weaned piglets and is a valuable reference for researchers engaged in animal health research to select stress models.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Enterotoxigenic Escherichia coli Challenge on Jejunal Morphology and Microbial Community Profiles in Weaned Crossbred Piglets

Xu,

Jia,

Xiao

et al. 2023

Microorganisms

View full text Add to dashboard Cite

show abstract

“…The focus was on the characteristics of the antimicrobial effect of substances secreted by Lactobacillus on clinical strains of P. mirabilis with the indication of the factor with the highest activity. On the basis of many studies, we expected that natural microbiota of the urinary tract which include Lactobacillus strains would positively affect the condition of the urinary system and have an inhibitory effect on the development of P. mirabilis infections [ 21 , 22 , 23 ]. Dysbiosis of the natural microbiota of the urinary tract is related to the development of infections.…”

Section: Discussionmentioning

confidence: 99%

Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

et al. 2022

View full text Add to dashboard Cite

The natural microbiota of the urinary tract includes Lactobacillus spp., which secrete molecules with antimicrobial properties and have antagonistic activity against many pathogens. This paper focuses on the antibacterial effect of Lactobacillus strains isolated from urine against clinical strains of Proteus mirabilis isolated from kidney stones and from urine with coexisting urolithiasis. The study involved analyzing the main antimicrobial molecules secreted by Lactobacillus. In order to indicate which agent had the strongest antimicrobial effect, the supernatants were made alkaline and treated with catalase and high temperature. Both treated and untreated supernatants were analyzed for their activity. Exposing uropathogens to all untreated cell-free supernatants of Lactobacillus significantly reduced their growth, and it was established that these properties were related to organic acid secretion by these strains. Using LC–MS/MS and spectrophotometric techniques, lactic, citric, and succinic acids were determined qualitatively and quantitatively. The influence of these acids on the P. mirabilis growth and biofilm formation and their influence on membrane permeability were also investigated. The results indicate that organic acids secreted by Lactobacillus strains have a high antibacterial potential and could be used as novel agents in the treatment of urinary tract infections caused by P. mirabilis.

show abstract

“…Although the urine biomass is extremely low, these frequent daily events would cause asymptomatic prostatic microenvironmental changes and chronic inflammation. Furthermore, pre-existing urinary dysbiosis can cause prostatic dysbiosis [ 10 , 20 , 21 ]. Although direct prostatic microbiome acquisition would be the best option for assessing the relationship between the microbiome and prostate cancer, such studies cannot be conducted easily because of their invasiveness and contamination risk.…”

Section: Direct Microbiome In Prostate Cancermentioning

confidence: 99%

Microbiome and Prostate Cancer: Emerging Diagnostic and Therapeutic Opportunities

Kim,

Park,

Choi

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

This review systematically addresses the correlation between the microbiome and prostate cancer and explores its diagnostic and therapeutic implications. Recent research has indicated an association between the urinary and gut microbiome composition and prostate cancer incidence and progression. Specifically, the urinary microbiome is a potential non-invasive biomarker for early detection and risk evaluation, with altered microbial profiles in prostate cancer patients. This represents an advancement in non-invasive diagnostic approaches to prostate cancer. The role of the gut microbiome in the efficacy of various cancer therapies has recently gained attention. Gut microbiota variations can affect the metabolism and effectiveness of standard treatment modalities, including chemotherapy, immunotherapy, and hormone therapy. This review explores the potential of gut microbiome modification through dietary interventions, prebiotics, probiotics, and fecal microbiota transplantation for improving the treatment response and mitigating adverse effects. Moreover, this review discusses the potential of microbiome profiling for patient stratification and personalized treatment strategies. While the current research identifies the pivotal role of the microbiome in prostate cancer, it also highlights the necessity for further investigations to fully understand these complex interactions and their practical applications in improving patient outcomes in prostate cancer management.

show abstract

Dysbiosis of the Human Urinary Microbiome and its Association to Diseases Affecting the Urinary System

Cited by 14 publications

References 90 publications

Effects of Enterotoxigenic Escherichia coli Challenge on Jejunal Morphology and Microbial Community Profiles in Weaned Crossbred Piglets

Effects of Enterotoxigenic Escherichia coli Challenge on Jejunal Morphology and Microbial Community Profiles in Weaned Crossbred Piglets

Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

Microbiome and Prostate Cancer: Emerging Diagnostic and Therapeutic Opportunities

Contact Info

Product

Resources

About