Dysfunctional nascent polypeptide-associated complex (NAC) activity in ribosomes enhances adriamycin toxicity in budding yeast

Takahashi, Tsutomu; Hirose, Ken; Mizutani, Emi; Naganuma, Akira

doi:10.2131/jts.34.703

Cited by 4 publications

(1 citation statement)

References 26 publications

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“…Screening of deletion collection strains for non-essential genes and studies with specific genes have revealed many genes and pathways in doxorubicin tolerance in yeast [ 14 , 15 ]. These include Ssl2 protein [ 16 ], Bsd2 protein [ 17 ], SUMO pathway [ 18 ], nascent polypeptide-associated complex activity in ribosomes [ 19 ], extracellular signal-regulated kinases ERK1 and ERK2 [ 13 ], endocytic Ark/Prk kinase [ 20 ], nitrogen permease regulator 2 ( Npr2 ) [ 21 ], cytochrome oxidase subunit IV gene [ 22 ], and overexpression of CLN1 , CLN2 and ERG13 [ 23 ]. Additionally, checkpoint and recombination functions in G1 and early S phase [ 14 ], as well as several proteins involved in DNA repair, RNA metabolism, chromatin remodeling, amino acid metabolism, and heat shock response [ 15 ], play roles in doxorubicin resistance.…”

Section: Introductionmentioning

confidence: 99%

High-Copy Overexpression Screening Reveals PDR5 as the Main Doxorubicin Resistance Gene in Yeast

Demir

Koç

2015

PLoS ONE

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Doxorubicin is one of the most potent anticancer drugs used in the treatment of various cancer types. The efficacy of doxorubicin is influenced by the drug resistance mechanisms and its cytotoxicity. In this study, we performed a high-copy screening analysis to find genes that play a role in doxorubicin resistance and found several genes (CUE5, AKL1, CAN1, YHR177W and PDR5) that provide resistance. Among these genes, overexpression of PDR5 provided a remarkable resistance, and deletion of it significantly rendered the tolerance level for the drug. Q-PCR analyses suggested that transcriptional regulation of these genes was not dependent on doxorubicin treatment. Additionally, we profiled the global expression pattern of cells in response to doxorubicin treatment and highlighted the genes and pathways that are important in doxorubicin tolerance/toxicity. Our results suggest that many efflux pumps and DNA metabolism genes are upregulated by the drug and required for doxorubicin tolerance.

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Section: Introductionmentioning

confidence: 99%

High-Copy Overexpression Screening Reveals PDR5 as the Main Doxorubicin Resistance Gene in Yeast

Demir

Koç

2015

PLoS ONE

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Exploring the roles of basal transcription factor 3 in eukaryotic growth and development

Jamil

Wang

et al. 2015

Biotechnology and Genetic Engineering Reviews

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Basal transcription factor 3 (BTF3) has been reported to play a significant part in the transcriptional regulation linking with eukaryotes growth and development. Alteration in the BTF3 gene expression patterns or variation in their activities adds to the explanation of different signaling pathways and regulatory networks. Moreover, BTF3s often respond to numerous stresses, and subsequently they are involved in regulation of various mechanisms. BTF3 proteins also function through protein-protein contact, which can assist us to identify the multifaceted processes of signaling and transcriptional regulation controlled by BTF3 proteins. In this review, we discuss current advances made in starting to explore the roles of BTF3 transcription factors in eukaryotes especially in plant growth and development.

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Inhibition of a Basal Transcription Factor 3-Like Gene Osj10gBTF3 in Rice Results in Significant Plant Miniaturization and Typical Pollen Abortion

Wang

Zhang

et al. 2012

Plant and Cell Physiology

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BTF3, which was originally recognized as a basal transcription factor, has been known to be involved in transcription initiation, translational regulation and protein localization in many eukaryotic organisms. However, its function remains largely unknown in plant species. In the present study, we analyzed a BTF3-related sequence in Oryza sativa L. subsp. japonica, which shares the conserved domain of a nascent polypeptide-associated complex with human BTF3, and was referred to as Osj10gBTF3. The expression of Osj10gBTF3 was primarily constitutive and generally modulated by salt, high temperature and exogenous phytohormone stress. The Osj10gBTF3::EGFP (enhanced green fluorescence protein) fusion protein was localized in both the nucleus and cytoplasmic membrane system. Inhibition of Osj10gBTF3 led to significant morphological changes in all detected tissues and organs, with a reduced size of between 25% and 52%. Furthermore, the pollen that developed was completely sterile, which was correlated with the altered expression of two Rf (fertility restorer)-like genes that encode pentatricopeptide repeat-containing proteins OsPPR676 and OsPPR920, translational initiation factors OseIF3e and OseIF3h, and the heat shock protein OsHSP82. These findings were verified through a yeast two-hybrid assay using a Nipponbare callus cDNA library as bait followed by the reverse transcription-PCR analysis of total leaf or anther RNAs. Our demonstration of the important role of Osj10gBTF3 in rice growth and development provides new insights showing that more complex regulatory functions are associated with BTF3 in plants.

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Dysfunctional nascent polypeptide-associated complex (NAC) activity in ribosomes enhances adriamycin toxicity in budding yeast

Cited by 4 publications

References 26 publications

High-Copy Overexpression Screening Reveals PDR5 as the Main Doxorubicin Resistance Gene in Yeast

High-Copy Overexpression Screening Reveals PDR5 as the Main Doxorubicin Resistance Gene in Yeast

Exploring the roles of basal transcription factor 3 in eukaryotic growth and development

Inhibition of a Basal Transcription Factor 3-Like Gene Osj10gBTF3 in Rice Results in Significant Plant Miniaturization and Typical Pollen Abortion

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