Dysregulated assembly of elastic fibers in fibulin-5 knockout mice results in a tendon-specific increase in elastic modulus

Eekhoff, Jeremy D.; Steenbock, Heiko; Berke, Ian M.; Brinckmann, Jürgen; Yanagisawa, Hiromi; Wagenseil, Jessica E.; Lake, Spencer P.

doi:10.1016/j.jmbbm.2020.104134

Cited by 9 publications

(3 citation statements)

References 58 publications

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“…Despite an initial assumption that the lower tendon modulus revealed by Javidi et al would primarily function to offset energy storage limitations inherent to possessing thick tendons in relation to body size, a morphological attribute which has been shown to decrease the capacity for energy storage and recovery during hopping (Biewener, 1997;Lichtwark and Wilson, 2006), our results suggest that an ideal tendon property has a stiffness that balances energy storage during hopping with optimal power generation during jumping. Additionally, due to a lack of understanding of the mechanical role of elastin in tendons, a growing body of literature hints at large variations in tendon structure and material properties, especially in tendons with high functional demands such as the ankle extensors (Eekhoff et al, 2017;Eekhoff et al, 2021). Going forward, to truly understand the species-level specificities generated by different tendon properties, our results broadly highlight the need to consider the diversity of mammalian tendon properties in future studies.…”

Section: Tendon Materials Propertiesmentioning

confidence: 74%

Elastic energy storage across speeds during steady-state hopping of desert kangaroo rats (Dipodomys deserti)

Christensen

Lin

Schwaner

et al. 2022

Journal of Experimental Biology

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Small bipedal hoppers, including kangaroo rats, are thought to not benefit from substantial elastic energy storage and return during hopping. However, recent species-specific material properties research suggests that, despite relative thickness, the ankle extensor tendons of these small hoppers are considerably more compliant than had been assumed. With faster locomotor speeds demanding higher forces, a lower tendon stiffness suggests greater tendon deformation and thus a greater potential for elastic energy storage and return with increasing speed. Using the elastic modulus values specific to kangaroo rat tendons, we sought to determine how much elastic energy is stored and returned during hopping across a range of speeds. In vivo techniques were used to record tendon force in the ankle extensors during steady-speed hopping. Our data support the hypothesis that the ankle extensor tendons of kangaroo rats store and return elastic energy in relation to hopping speed, storing more at faster speeds. Despite storing comparatively less elastic energy than larger hoppers, this relationship between speed and energy storage offer novel evidence of a functionally similar energy storage mechanism, operating irrespective of body size or tendon thickness, across the distal muscle-tendon units of both small and large bipedal hoppers.

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Section: Tendon Materials Propertiesmentioning

confidence: 74%

Elastic energy storage across speeds during steady-state hopping of desert kangaroo rats (Dipodomys deserti)

Christensen

Lin

Schwaner

et al. 2022

Journal of Experimental Biology

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“…The clamps for mechanical test were fixed in a 60 mm Petri dish with glue and gripped fresh samples, which were at the same horizontal line without stress. The samples were imaged in pure water, and the collagen fibers were visualized by second harmonic generation (SHG, excitation: 840nm; emission: 410–460 nm) ( Eekhoff et al, 2020 ). Randomly select a field of view.…”

Section: Methodsmentioning

confidence: 99%

The alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestion

Liu,

Deng,

Liang

et al. 2024

Front. Bioeng. Biotechnol.

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Background: The treatment of patellar tendon injury has always been an unsolved problem, and mechanical characterization is very important for its repair and reconstruction. Elastin is a contributor to mechanics, but it is not clear how it affects the elasticity, viscoelastic properties, and structure of patellar tendon.Methods: The patellar tendons from six fresh adult experimental pigs were used in this study and they were made into 77 samples. The patellar tendon was specifically degraded by elastase, and the regional mechanical response and structural changes were investigated by: (1) Based on the previous study of elastase treatment conditions, the biochemical quantification of collagen, glycosaminoglycan and total protein was carried out; (2) The patellar tendon was divided into the proximal, central, and distal regions, and then the axial tensile test and stress relaxation test were performed before and after phosphate-buffered saline (PBS) or elastase treatment; (3) The dynamic constitutive model was established by the obtained mechanical data; (4) The structural relationship between elastin and collagen fibers was analyzed by two-photon microscopy and histology.Results: There was no statistical difference in mechanics between patellar tendon regions. Compared with those before elastase treatment, the low tensile modulus decreased by 75%–80%, the high tensile modulus decreased by 38%–47%, and the transition strain was prolonged after treatment. For viscoelastic behavior, the stress relaxation increased, the initial slope increased by 55%, the saturation slope increased by 44%, and the transition time increased by 25% after enzyme treatment. Elastin degradation made the collagen fibers of patellar tendon become disordered and looser, and the fiber wavelength increased significantly.Conclusion: The results of this study show that elastin plays an important role in the mechanical properties and fiber structure stability of patellar tendon, which supplements the structure-function relationship information of patellar tendon. The established constitutive model is of great significance to the prediction, repair and replacement of patellar tendon injury. In addition, human patellar tendon has a higher elastin content, so the results of this study can provide supporting information on the natural properties of tendon elastin degradation and guide the development of artificial patellar tendon biomaterials.

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“…Collagen I fibrils play a dominant role in dictating tendon mechanical strength; however, these fibrils are tightly regulated by other collagens and non-collagenous proteins present in tendon [4] , [5] , [6] . Previous studies have shown that matrix molecules interact to properly organize collagen I fibrils and maintain tendon mechanical integrity [7] , [8] , [9] , [10] . The fundamental mechanisms by which these ECM components cooperatively maintain tendon function, however, are unknown.…”

Section: Introductionmentioning

confidence: 99%

Coordinate roles for collagen VI and biglycan in regulating tendon collagen fibril structure and function

Leiphart

Pham

Harvey

et al. 2022

Matrix Biology Plus

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Highlights Biglycan and collagen VI co-localize within nascent tendon matrix indicating possible synergy in tissue function. Biglycan deficiency reduced tendon fibril size and mechanical properties, but collagen VI knockout led to further reductions in these properties. Tendons deficient in both collagen VI and biglycan did not display further reductions in tissue properties. Biglycan and collagen VI play distinct and non-additive roles in regulating tendon function.

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Dysregulated assembly of elastic fibers in fibulin-5 knockout mice results in a tendon-specific increase in elastic modulus

Cited by 9 publications

References 58 publications

Elastic energy storage across speeds during steady-state hopping of desert kangaroo rats (Dipodomys deserti)

Elastic energy storage across speeds during steady-state hopping of desert kangaroo rats (Dipodomys deserti)

The alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestion

Coordinate roles for collagen VI and biglycan in regulating tendon collagen fibril structure and function

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