Dysregulation in the expression of (lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283) in spinal cord injury

Salah, Sara Mohammed Mohammed; Matboli, Marwa; Nasser, Hebatallah A.; Abdelnaiem, Ibrahim Abdelmohsen; Shafei, Ayman; El-Asmer, Mohamed

doi:10.1016/j.ygeno.2020.06.018

Cited by 12 publications

(12 citation statements)

References 38 publications

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“…In a previous study, Sara et al found that SCI patients had a higher level of expression of the lncRNA TSIX than the controls. [ 15 ] qRT‐PCR analysis was used to detect the expression of the lncRNA TSIX in the serum of SCI patients and spinal cord tissues of SCI mice. The results revealed that patients with SCI had significantly higher levels of lncRNA TSIX expression than the control group ( p < 0.01) (Figure 1A).…”

Section: Resultsmentioning

confidence: 99%

“…In a previous study, Sara et al found that SCI patients had a higher level of expression of the lncRNA TSIX than the controls. [15] qRT-PCR analysis was used to detect the expression of the lncRNA TSIX in the serum of SCI patients and spinal cord tissues of SCI mice.…”

Section: Lncrna Tsix Was Significantly Upregulated Following Scimentioning

confidence: 99%

“…[ 13 ] LncRNA X‐inactive‐specific transcript (TSIX) is the lncRNA antisense for XIST and exerts negative regulation on XIST expression. [ 14 ] Sara et al have previously reported that SCI patients have an enhanced level of lncRNA‐TSIX mRNA expression compared with the control group, [ 15 ] demonstrating that lncRNA‐TSIX may play critical functions in SCI. However, few studies have elucidated the function and underlying mechanism of lncRNA‐TSIX in SCI development.…”

Section: Introductionmentioning

confidence: 99%

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LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR‐532‐3p/DDOST axis

Dong

Wei

Zhu

2023

J Biochem & Molecular Tox

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Long noncoding RNA (lncRNA)‐X‐inactive‐specific transcript (TSIX) expression is upregulated in spinal cord tissues following spinal cord injury (SCI). However, the role of lncRNA‐TSIX in SCI remains elusive. SCI animal model was established using C57BL/6 mice. LncRNA TSIX and miR‐532‐3p expression were determined using quantitative reverse transcription polymerase chain reaction (qRT‒PCR). Apoptosis, cell proliferation, and migration were evaluated by transferase dUTP nick end labeling staining, CCK‐8, and Transwell assays, respectively. The interaction of miR‐532‐3p with lncRNA TSIX and DDOST was explored via a dual‐luciferase reporter system. Hematoxylin‐eosin staining and the Basso, Beattie, and Bresnahan locomotor rating (BBB) scale were performed to investigate SCI progression. The expression of the lncRNA TSIX was found to be significantly upregulated in the serum of SCI patients and spinal cord tissues of SCI mice. The overexpression of lncRNA TSIX enhanced spinal cord neural stem cell (SC‐NSC) proliferation and migration in vitro while inhibiting apoptosis and inflammatory cell infiltration in vivo. Moreover, lncRNA TSIX acted as a molecular sponge for miR‐532‐3p, and the knockdown of miR‐532‐3p promoted proliferation and migration and inhibited apoptosis of SC‐NSCs. Moreover, DDOST was found to be the downstream target of miR‐532‐3p, and DDOST overexpression showed a similar effect as miR‐532‐3p silencing on the proliferation, migration, and apoptosis of SC‐NSCs. Furthermore, we found that lncRNA TSIX overexpression promoted the activation of the PI3K/AKT signaling pathway. LncRNA TSIX aggravates SCI by regulating the PI3K/AKT pathway via the miR‐532‐3p/DDOST axis, indicating potential applications for targeted therapy of SCI regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Lncrna Tsix Was Significantly Upregulated Following Scimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR‐532‐3p/DDOST axis

Dong

Wei

Zhu

2023

J Biochem & Molecular Tox

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“…et al, 2018). Additionally, clinical results indicated that SCI patients' serum lncRNA-tSix levels are slightly greater than the control groups, which means that lncRNA-tSix can potentially be a biomarker for SCI diagnosis (Salah et al, 2020). As a result, exosomes that overexpress various lncRNAs may provide for their possible development as novel treatments for SCI and might perform a vital role in the future.…”

Section: Exosomes As Drug Delivery Vehicles For Ncrnasmentioning

confidence: 99%

Emerging Exosomes and Exosomal MiRNAs in Spinal Cord Injury

Feng

Zhang

Zhu

et al. 2021

Front. Cell Dev. Biol.

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Acute spinal cord injury (SCI) is a serious traumatic event to the spinal cord with considerable morbidity and mortality. This injury leads to short- and long-term variations in the spinal cord, and can have a serious effect on the patient’s sensory, motor, or autonomic functions. Due to the complicated pathological process of SCI, there is currently no successful clinical treatment strategy. Exosomes, extracellular vesicles (EVs) with a double-layer membrane structure of 30–150 nm diameter, have recently been considered as critical mediators for communication between cells and tissues by transferring proteins, lipids, and nucleic acids. Further studies verified that exosomes participate in the pathophysiological process of several diseases, including cancer, neurodegenerative diseases, and cardiovascular diseases, and could have a significant impact in their treatment. As natural carriers of biologically active cargos, exosomes have emerged as pathological mediators of SCI. In this review article, we critically discuss the functions of exosomes as intracellular mediators and potential treatments in SCI and provide an outlook on future research.

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“…Moreover, lncRNAs also serve as a crucial regulator in SCI, affecting its initiation and progression. Several studies have demonstrated that they mostly perform functions via cell autophagy and apoptosis (Salah et al, 2020). However, some evidence tells us that lncRNAs probably also play a role in regulating axonal regrowth following SCI.…”

Section: Non-coding Rnas In Central Nerve Regenerationmentioning

confidence: 99%

Roles of Non-coding RNAs in Central Nervous System Axon Regeneration

Jia

Tang

et al. 2021

Front. Neurosci.

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Axons in the central nervous system often fail to regenerate after injury due to the limited intrinsic regeneration ability of the central nervous system (CNS) and complex extracellular inhibitory factors. Therefore, it is of vital importance to have a better understanding of potential methods to promote the regeneration capability of injured nerves. Evidence has shown that non-coding RNAs play an essential role in nerve regeneration, especially long non-coding RNA (lncRNA), microRNA (miRNA), and circular RNA (circRNA). In this review, we profile their separate roles in axon regeneration after CNS injuries, such as spinal cord injury (SCI) and optic nerve injury. In addition, we also reveal the interactive networks among non-coding RNAs.

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Dysregulation in the expression of (lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283) in spinal cord injury

Cited by 12 publications

References 38 publications

LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR‐532‐3p/DDOST axis

LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR‐532‐3p/DDOST axis

Emerging Exosomes and Exosomal MiRNAs in Spinal Cord Injury

Roles of Non-coding RNAs in Central Nervous System Axon Regeneration

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