Dysregulation of CD1d-Restricted Type II Natural Killer T Cells Leads to Spontaneous Development of Colitis in Mice

Liao, Chia–Min; Zimmer, Michael I.; Shanmuganad, Sharmila; Yu, Hon‐Tsen; Cardell, Susanna; Wang, Chyung–Ru

doi:10.1053/j.gastro.2011.10.030

Cited by 58 publications

(69 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The plaque area was quantified from 3 aortic root sections as described previously (51) using Nikon-Elements software. For colon sections, the organ was isolated as previously described (58) and fixed in 10% formalin and laid out in Swiss rolls for paraffin embedding. Paraffin sections were cut and stained with H&E for examination of the tissue architecture.…”

Section: Methodsmentioning

confidence: 99%

CD1b-autoreactive T cells contribute to hyperlipidemia-induced skin inflammation in mice

Bagchi¹,

Hé²,

Zhao³

et al. 2017

Journal of Clinical Investigation

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

CD1b-autoreactive T cells contribute to hyperlipidemia-induced skin inflammation in mice

Bagchi¹,

Hé²,

Zhao³

et al. 2017

Journal of Clinical Investigation

View full text Add to dashboard Cite

“…Because of the lack of a direct method to identify type II NKT cells in vivo, the overall knowledge of type II NKT cells remains very limited. However, type II NKT cells are more prevalent than type I NKT cells in humans and are known to accumulate and play a role in several diseases, such as ulcerative colitis, hepatitis, and multiple myeloma (4)(5)(6)(7)(8)(9). Therefore, a more in-depth characterization of type II NKT cells is warranted to better understand their regulation and function in health and disease.…”

mentioning

confidence: 99%

Polyclonal type II natural killer T cells require PLZF and SAP for their development and contribute to CpG-mediated antitumor response

Zhao

Weng

Bagchi

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

CD1d-restricted natural killer T (NKT) cells are innate-like T cells with potent immunomodulatory function via rapid production of both Th1 and Th2 cytokines. NKT cells comprise well-characterized type I NKT cells, which can be detected by α-galactosylceramide-loaded CD1d tetramers, and less-studied type II NKT cells, which do not recognize α-galactosylceramide. Here we characterized type II NKT cells on a polyclonal level by using a Jα18-deficient IL-4 reporter mouse model. This model allows us to track type II NTK cells by the GFP + TCRβ + phenotype in the thymus and liver. We found type II NKT cells, like type I NKT cells, exhibit an activated phenotype and are dependent on the transcriptional regulator promyelocytic leukemia zinc finger (PLZF) and the adaptor molecule signaling lymphocyte activation molecule-associated protein (SAP) for their development. Type II NKT cells are potently activated by β-D -glucopyranosylceramide (β-GlcCer) but not sulfatide or phospholipids in a CD1d-dependent manner, with the stimulatory capacity of β-GlcCer influenced by acyl chain length. Compared with type I NKT cells, type II NKT cells produce lower levels of IFN-γ but comparable amounts of IL-13 in response to polyclonal T-cell receptor stimulation, suggesting they may play different roles in regulating immune responses. Furthermore, type II NKT cells can be activated by CpG oligodeoxynucletides to produce IFN-γ, but not IL-4 or IL-13. Importantly, CpG-activated type II NKT cells contribute to the antitumor effect of CpG in the B16 melanoma model. Taken together, our data reveal the characteristics of polyclonal type II NKT cells and their potential role in antitumor immunotherapy.

show abstract

“…CD4+ provides an auxiliary function in antigen-recognition receptors, while CD45+ is expressed in many kind of cells including blood and tumor cells and primarily in endothelial cells, and affects the differentiation of hematopoietic stem cells and the generation and metastasis of tumor cells (Liao et al, 2012). CD4+CD45+ T cells mainly assist the B cells.…”

Section: Discussionmentioning

confidence: 99%

Expression and clinical significance of CD4+ CD45+ peripheral blood T cells in patients with ulcerative colitis

Guo¹,

Li²,

Jin³

2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The objectives of this study were to explore the expression of peripheral blood CD4+CD45+ T cells in patients with ulcerative colitis (UC) and determine its clinical value. We selected 80 patients with UC from the First Affiliated Hospital of Liaoning Medical University from March 2012 to December 2013. Of these, 27 had mildly active, 28 moderately active, and 25 severely active UC. We also recruited 80 subjects to constitute the healthy control group. The percentages of CD4+CD45+ molecules on the peripheral blood T cell surfaces of patients were detected using flow cytometry and were compared between patients to determine the severity of illness. The percentage of peripheral blood CD4+CD45+T cells in the UC group was 52.93 ± 3.64% and in the controls it was 41.34 ± 2.94%; the UC group percentages were significantly higher (t = -22.159, P < 0.05). The average percentages in patients with mild, moderate, and severe activity were 50.99 ± 1.45, 52.66 ± 1.41, and 55.18 ± 2.18%, respectively; the moderate activity percentage was higher than that of mild activity, and the severely active stage percentage was overall the highest. Comparison between groups showed a statistically significant 10339 Peripheral blood CD4+ CD45+ T cells in ulcerative colitis ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 10338-10343 (2015) difference, F = 39.850, (P < 0.05). The expression levels of peripheral blood CD4+CD45+ T cells in the UC group were higher than those in the control group. Overall, our results showed that with the aggravation of disease the peripheral blood CD4+CD45+ T cell percentages were significantly increased, which might be useful as a marker for clinical diagnosis.

show abstract

Dysregulation of CD1d-Restricted Type II Natural Killer T Cells Leads to Spontaneous Development of Colitis in Mice

Cited by 58 publications

References 35 publications

CD1b-autoreactive T cells contribute to hyperlipidemia-induced skin inflammation in mice

CD1b-autoreactive T cells contribute to hyperlipidemia-induced skin inflammation in mice

Polyclonal type II natural killer T cells require PLZF and SAP for their development and contribute to CpG-mediated antitumor response

Expression and clinical significance of CD4+ CD45+ peripheral blood T cells in patients with ulcerative colitis

Contact Info

Product

Resources

About