Dysregulation of DNA Methylation in the Aryl-Hydrocarbon Receptor Repressor (&lt;i&gt;AHRR&lt;/i&gt;) Gene

Nomura, Seitaro; Morita, Hiroyuki

doi:10.1253/circj.cj-22-0111

Cited by 1 publication

(1 citation statement)

References 13 publications

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“…In this study, we replicated many previously reported sites, including those annotated to AHRR , RARA , F2RL3 , PRSS23 and GFI1 [ 31 ], and identified a substantial number of the novel smoking-associated candidates by using the latest EPIC BeadChip. The AHRR gene consistently appeared as the most significantly affected genomic locus in studies investigating the impact of smoking [ 32 , 33 ], a pattern also evident in our cohort. Specifically, 41 DMPs associated with current smoking were annotated to AHRR in the 5mC+5hmC dataset, and 11 in the 5mC dataset.…”

Section: Discussionsupporting

confidence: 73%

Smoking-Induced DNA Hydroxymethylation Signature Is Less Pronounced than True DNA Methylation: The Population-Based KORA Fit Cohort

Lai,

Matías-García,

Kretschmer

et al. 2024

Biomolecules

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Despite extensive research on 5-methylcytosine (5mC) in relation to smoking, there has been limited exploration into the interaction between smoking and 5-hydroxymethylcytosine (5hmC). In this study, total DNA methylation (5mC+5hmC), true DNA methylation (5mC) and hydroxymethylation (5hmC) levels were profiled utilizing conventional bisulphite (BS) and oxidative bisulphite (oxBS) treatment, measured with the Illumina Infinium MethylationEPIC BeadChip. An epigenome-wide association study (EWAS) of 5mC+5hmC methylation revealed a total of 38,575 differentially methylated positions (DMPs) and 2023 differentially methylated regions (DMRs) associated with current smoking, along with 82 DMPs and 76 DMRs associated with former smoking (FDR-adjusted p < 0.05). Additionally, a focused examination of 5mC identified 33 DMPs linked to current smoking and 1 DMP associated with former smoking (FDR-adjusted p < 0.05). In the 5hmC category, eight DMPs related to current smoking and two DMPs tied to former smoking were identified, each meeting a suggestive threshold (p < 1 × 10−5). The substantial number of recognized DMPs, including 5mC+5hmC (7069/38,575, 2/82), 5mC (0/33, 1/1), and 5hmC (2/8, 0/2), have not been previously reported. Our findings corroborated previously established methylation positions and revealed novel candidates linked to tobacco smoking. Moreover, the identification of hydroxymethylated CpG sites with suggestive links provides avenues for future research.

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Section: Discussionsupporting

confidence: 73%

Smoking-Induced DNA Hydroxymethylation Signature Is Less Pronounced than True DNA Methylation: The Population-Based KORA Fit Cohort

Lai,

Matías-García,

Kretschmer

et al. 2024

Biomolecules

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Dysregulation of DNA Methylation in the Aryl-Hydrocarbon Receptor Repressor (<i>AHRR</i>) Gene

Abstract: Figure. Mechanistic model showing that smoking-or PTSD-related activation of AhR signaling promotes inflammation and accelerates the development of cardiovascular diseases via DNA hypomethylation of AHRR. PTSD, post-traumatic stress disorder.

Cited by 1 publication

References 13 publications

Smoking-Induced DNA Hydroxymethylation Signature Is Less Pronounced than True DNA Methylation: The Population-Based KORA Fit Cohort

Smoking-Induced DNA Hydroxymethylation Signature Is Less Pronounced than True DNA Methylation: The Population-Based KORA Fit Cohort

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