Dysregulation of Intestinal Microbiota Elicited by Food Allergy Induces IgA-Mediated Oral Dysbiosis

Matsui, Shohei; Kataoka, Hideo; Tanaka, J; Kikuchi, Masatoshi; Fukamachi, Hiromi; Morisaki, Hirobumi; Matsushima, Hitomi; Mishima, Kenji; Hironaka, Shouji; Takaki, Takashi; Okahashi, Nobuo; Maruoka, Yasubumi; Kuwata, Hirotaka

doi:10.1128/iai.00741-19

Cited by 15 publications

(14 citation statements)

References 42 publications

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“…This result was consistent with the IL-33 expression profile of C. koseri reported in a previous study using an intestinal epithelial cell line [6]. Next, whether the IL-33-inducing ability of C.…”

Section: Resultssupporting

confidence: 92%

“…koseri JCM1658 was obtained from the Cell Engineering Division of RIKEN BioResource Center. A strain of Enterococcus gallinarum isolated from mouse faeces was cultured as described previously [6]. A common Escherichia coli strain, K-12, was used.…”

Section: Methodsmentioning

confidence: 99%

“…Multiple bacterial orders of multiple bacteria, most commonly Clostridiales and Lactobacillales as well as Bacteroidales and Enterobacterales , are thought to be implicated in the development of FAs [3, 5]. The intestinal microbiome of a murine model of FAs identified several bacterial characteristics of FAs [6]. Importantly, Citrobacter species, Gram-negative facultative anaerobes of Enterobacteriaceae that utilize citrate as their sole carbon source [7], were found to be especially abundant in FA mice, and could stimulate expression of the allergenic cytokine IL-33 in intestinal epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, Citrobacter species, Gram-negative facultative anaerobes of Enterobacteriaceae that utilize citrate as their sole carbon source [7], were found to be especially abundant in FA mice, and could stimulate expression of the allergenic cytokine IL-33 in intestinal epithelial cells. Moreover, orally administered Citrobacter koseri was found to exacerbate allergic symptoms by reducing the number of intestinal Th17 cells [6]. Therefore, the dysbiotic abundance of Citrobacter species in the intestinal microbiome is believed to be associated with the development of FAs.…”

Section: Introductionmentioning

confidence: 99%

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Citrobacter koseri stimulates dendritic cells to induce IL-33 expression via abundant ATP production

Kataoka

Mori

Into

2021

Journal of Medical Microbiology

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Introduction. Food allergies (FAs) occur due to intestinal immune dysfunction elicited by dysbiotic conditions. It was previously determined by us that Citrobacter species propagate in the faeces of mice with FAs and worsen allergic symptoms by inducing the allergenic cytokine IL-33. Dendritic cells can play important roles in regulation of FA responses. Hypothesis. Citrobacter species propagating in intestines of mice worsen allergic symptoms by stimulating dendritic cells to induce IL-33 expression. Aim. The aim of the present study was to analyse whether C. koseri stimulates dendritic cells to induce IL-33 expression. Methodology. IL-33 expression was evaluated in a DC2.4 mouse dendritic cell line stimulated by live or heat-inactivated C. koseri JCM1658, ATP, LPS extracted from C. koseri JCM1658 or other enterobacteria by real-time PCR. The ATP concentration and number of live bacteria in the culture supernatant were measured simultaneously. Results. Live C. koseri JCM1658 induced higher levels of IL-33 expression than other enterobacteria tested, but such a response was not elicited by heat-inactivated C. koseri JCM1658. LPS extracted from C. koseri JCM1658 did not induce IL-33 expression and suppressed live C. koseri JCM1658-induced IL-33 expression via the activation of Toll-like receptor 4 signalling. Furthermore, ATP produced by C. koseri JCM1658 stimulated dendritic cells to induce IL-33 expression by stimulating the P2X7 receptor, and LPS attenuated extracellular ATP-induced IL-33 expression. C. koseri JCM1658 was observed to proliferate more vigorously and produce more ATP than other enterobacteria. Conclusion. C. koseri acts as an allergenic bacterium through ATP production, stimulating dendritic cells to induce IL-33 expression, while LPS released from inactivated C. koseri JCM1658 attenuates this allergenicity.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Citrobacter koseri stimulates dendritic cells to induce IL-33 expression via abundant ATP production

Kataoka

Mori

Into

2021

Journal of Medical Microbiology

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“…Also, many microbes are associated with the immune response and therapeutic results in gut inflammation. For example, oral administration of Citrobacter koseri JCM1658 aggravates systemic allergic reactions and reduced numbers of intestinal T-helper-17 cells (Matsui et al, 2019).…”

Section: Key Microbiota and Network Revealed The Specific Pathogenesis Underlying Small-bowel Inflammation And Cgmentioning

confidence: 99%

Rewiring of Microbiota Networks in Erosive Inflammation of the Stomach and Small Bowel

Chen

Fan

Zhang

et al. 2020

Front. Bioeng. Biotechnol.

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The development of non-invasive, inexpensive, and effective early diagnosis tests for gastric and small-bowel lesions is an urgent requirement. The introduction of magnetically guided capsule endoscopy (MGCE) has aided examination of the small bowel for diagnoses. However, the distribution of the fecal microbiome in abnormal erosions of the stomach and small bowel remains unclear. Herein, alternations in the fecal microbiome in three groups [normal, small-bowel inflammation, and chronic gastritis (CG)] were analyzed by metagenomics and our well-developed method [individual-specific edge-network analysis (iENA)]. In addition to the dominant microbiota identified by the conventional differential analysis, iENA could recognize novel network biomarkers of microbiome communities, such as the genus Bacteroide in CG and small-bowel inflammation. Combined with differential network analysis, the network-hub microbiota within rewired microbiota networks revealed high-ranked iENA microbiota markers, which were disease specific and had particular pathogenic functions. Our findings illuminate the components of the fecal microbiome and the importance of specific bacteria in CG and small-bowel erosions, and could be employed to develop preventive and non-invasive therapeutic strategies.

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Preventive Effects of Probiotics and Prebiotics in Food Allergy: Potentials and Promise

Shahali

Dadar

2021

Microorganisms for Sustainability

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Dysregulation of Intestinal Microbiota Elicited by Food Allergy Induces IgA-Mediated Oral Dysbiosis

Cited by 15 publications

References 42 publications

Citrobacter koseri stimulates dendritic cells to induce IL-33 expression via abundant ATP production

Citrobacter koseri stimulates dendritic cells to induce IL-33 expression via abundant ATP production

Rewiring of Microbiota Networks in Erosive Inflammation of the Stomach and Small Bowel

Preventive Effects of Probiotics and Prebiotics in Food Allergy: Potentials and Promise

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