Dysregulation of miR-192-5p in acute pancreatitis patients with nonalcoholic fatty liver and its functional role in acute pancreatitis progression

Abstract

Background: Nonalcoholic fatty liver disease (NAFLD) is a frequent metabolic disease and has been demonstrated to contribute to the severity of acute pancreatitis (AP). The present study aimed to investigate the aberrant expression of microRNA-192-5p (miR-192-5p) in AP patients with NAFLD, and further analyze the clinical significance and biological function of miR-192-5p in AP progression. Methods: Expression of miR-192-5p was estimated using quantitative real-time PCR (qRT-PCR). Diagnostic value of miR-192-5p was evaluated by the receiver operating characteristic curve (ROC). The effects of miR-192-5p on cell proliferation, apoptosis and inflammatory response of pancreatic acinar cells were further assessed by CCK-8 assay, flow cytometry and enzyme-linked immunosorbent assay (ELISA). Results: Circulating miR-192-5p was decreased in AP patients with NAFLD compared with those patients without NAFLD and healthy controls (P<0.05). The down-regulated expression of miR-192-5p had a relative high diagnostic accuracy to distinguish the AP patients with NAFLD from the cases without NAFLD. Furthermore, the overexpression of miR-192-5p in pancreatic acinar cells led to the decreased cell proliferation, increased cell apoptosis and suppressed inflammatory reaction (all P<0.05). Conclusion: Collectively, all data indicated that serum expression of miR-192-5p in AP patients with NAFLD is significantly decreased and serves as a candidate diagnostic biomarker. The up-regulation of miR-192-5p in pancreatic acinar cell leads to increased cell apoptosis and decreased inflammatory response, suggesting the potential of miR-192-5p as a therapeutic target of AP.

MiR-192-5p Alleviated Fibrosis and Inflammatory Responses of Tendon Cells by Targeting NFAT5

“…Studies have shown that hepatocellular-derived exosome miR-194-5p can regulate the Rictor/Akt/FoxO1 signaling pathway, thus playing a major role in the progression of non-alcoholic fatty liver disease [38]. Hu et al found that the upregulation of miR-192-5p in pancreatic acinar cells can reduce the inflammatory response [39]. MiR-192-5p can reduce the inflammatory 7 Computational and Mathematical Methods in Medicine response in both the liver and pancreas.…”

Deciphering the role of epigenetic modifications in fatty liver disease: A systematic review

“…MiR‐192 was reported by three studies 26,40,43 showing that circulating miR‐192 is upregulated in participants with NAFLD, NASH or simples steatosis than healthy controls, only one study 36 suggested that serum expression of miR‐192‐5p in patients with acute pancreatitis and NAFLD is significantly down‐regulated compared to acute pancreatitis patients without NAFLD and healthy controls.…”

“…5 We found 24 studies 2,6-9,26-43 that investigated the association of miRNAs with FLD (Table 2). Of these, 13 studies used blood samples, 6,[26][27][28][29][30][31][32][33][34][35][36] three studies used liver tissue 2,37,38 and eight studies used both blood and liver tissue samples. [7][8][9][39][40][41][42][43] In addition, the studies included population with Asian (n = 9), European (n = 7), North American (n = 6) and South American (n = 2) ancestries with mean age of 46.9 years old.…”

Fighting Fire with Fire: Exosomes and Acute Pancreatitis-Associated Acute Lung Injury

“…This result is similar to that of previous studies. Patients with AP with nonalcoholic fatty liver disease had significantly lower circulating miR-192-5p levels than the healthy control group [ 101 ]. Furthermore, the level of exosome-specific miR-122-5p was upregulated only in the SAP group but not in the MAP group [ 97 ].…”