Dysregulation of miR-551b-5p and SETD2 Predicts Poor Prognosis and Promotes Migration and Invasion of Thyroid Cancers

Abstract

Objective:: This study was to investigate the clinical significance of miR-551b-5p and SETD2 in thyroid cancers (TC) and their effects on the biological function of TC cells Methods:: The expression level of miR-551b-5p and SETD2 in tumor/nontumor tissues and TC cell lines was measured by quantitative real-time polymerase chain reaction (RT-qPCR). Subsequently, the relationship between miR-551b-5p or SETD2 expression and the clinicopathological feature was detected by Chi-square analysis. Kaplan-Meier and multivariate Cox regression analyses were used to assess their prognostic values. Finally, the regulatory effects of miR-551b-5p and SETD2 on the proliferation, migration and invasion ability of TC cells were detected by CCK-8 and Transwell assays. Results:: Compared with non-tumor groups, the expression of miR-551b-5p was significantly increased in patients' tissues and TC cell lines, while SETD2 mRNA expression was decreased. Patients with up-regulated miR-551b-5p or downregulated SETD2 mRNA in TC showed more positive lymph node metastasis and advanced TNM stage. High miR-551b-5p expression level and low SETD2 mRNA level were related to poor survival rate. miR-551b-5p and SETD2 might be potential prognostic biomarkers for TC. miR-551b-5p knockdown can inhibit cell proliferation, migration and invasion by targeting SETD2 Conclusion:: miR-551b-5p and SETD2 may be valuable prognostic biomarkers and new therapeutic targets for TC. result: Compared with all control groups, the expression of miR-551b-5p was significantly increased in 133 patients'' tissues and TC cell lines, while SETD2 mRNA expression was decreased. Patients with up-regulated miR-551b-5p and downregulated SETD2 mRNA in TC showed more positive lymph node metastasis and advanced TNM stage. High miR-551b-5p expression level and low SETD2 mRNA level were related to poor survival rate. miR-551b-5p and SETD2 might be potential prognostic biomarkers for TC. miR-551b-5p knockdown can inhibit cell migration and invasion by targeting SETD2. other: N/A

A comprehensive analysis of the prognostic characteristics of microRNAs in breast cancer

“…miR-551b is located on chromosome 3q26.2 and participates in a variety of biological processes, such as inflammatory reaction, carcinogenic, chemoresistance ( Chaluvally-Raghavan et al, 2014 ; Wei et al, 2016 ; Zhang et al, 2018 ). In thyroid cancers, miR-551b expression levels were correlated with lymph node metastasis, and TNM stage ( Dong et al, 2023 ). And miR-551b can be an independent prognostic factor with lower overall survival and worse prognosis of lung adenocarcinoma ( Lin et al, 2016 ).…”