E-cadherin, N-cadherin Expression and Histologic Characterization of Canine Choroid Plexus Tumors

Abstract

Choroid plexus tumors (CPTs) are reported with an increasing incidence in dogs, and they call for a reexamination of histologic features and criteria of classification corresponding to their biological behavior. In this study, the human World Health Organization classification was applied to 16 canine CPTs, and the expression of molecules involved in neoplastic cell adhesion (E-cadherin, N-cadherin), invasion (doublecortin), and proliferation (Ki-67) was investigated. Mitotic index was found to be the main criterion for grading CPTs. Cell density and multilayering of papillae were also statistically associated with histologic grade. Intraventricular spread and parenchymal invasion was observed for tumors showing histologic benign features. E-cadherin was expressed in all CPT grades, independent of tumor invasion. N-cadherin immunolabeling was more expressed in grade I than high-grade CPTs, whereas doublecortin expression was not detected in CPTs. An increasing proliferative activity was observed in relation with histologic grade.

Clinicopathologic Features, Diagnosis, and Characterization of the Immune Cell Population in Canine Choroid Plexus Tumors

“…This assessment can be especially problematic when examining biopsy samples, which typically have a limited amount of tissue available (25). IHC for cytokeratin, glial fibrillary acidic protein (GFAP), E-cadherin, N-cadherin, and beta-catenin can be useful to support a diagnosis of CPT, but these immunomarkers lack specificity and often provide inconsistent results (26–28). Here we confirm that Kir7.1 is a reliable antibody for diagnostic confirmation of choroid plexus neoplasms in dogs.…”

Canine Primary Intracranial Cancer: A Clinicopathologic and Comparative Review of Glioma, Meningioma, and Choroid Plexus Tumors

“…Vascular endothelial growth factor (VEGF), platelet-derived growth factor and it associated receptors, PDGFRα, and PDGFRβ exhibit labeling commonly in CPTs regardless of the designation of papilloma or carcinoma (144). Doublecortin labeling has not been recorded in these tumors (150).…”

“…E-cadherin and β-catenin labeling are commonly observed in canine choroid plexus tumors and can be associated with aberrant cytoplasmic or nuclear localization (149). While E-cadherin labeling does not vary between benign and malignant CPTs, the related protein N-cadherin has been shown to be localized more commonly to choroid plexus papillomas than carcinomas (150). Immunolabeling for glial fibrillary acidic protein (GFAP) has also been recorded in canine CPTs; however, this is not a specific marker of choroid plexus cells (149).…”

Role of Cadherins in Cancer—A Review

“…In tumor progression, it has been documented that abnormal expression of N-cadherin is connected with malignancies manifested by cell transformation, apoptosis, angiogenesis, invasion and metastasis [ 89 ]. Conversely, in canine choroid plexus tumors, N-cadherin immunolabeling was more expressed in grade I tumors [ 95 ]. In feline adenomas and carcinomas, N-cadherin expression is associated with a reduced expression of E-cadherin and the presence of regional metastasis [ 96 ].…”