E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork

Campbell, Joseph L.; Kleckner, Nancy

doi:10.1016/0092-8674(90)90271-f

Cited by 444 publications

(518 citation statements)

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“…Function of sequestration of oriC by SeqA also has to be considered (Campbell and Kleckner, 1990;Crooke, 1995). SeqA prolongs the time that oriC is in the hemimethylated state (Lu et al, 1994), hemimethylated oriC forms a complex with the cell membrane (Ogden et al, 1988) and initiation is inhibited (Messer et al, 1985;Smith et al, 1985;Russell and Zinder, 1987).…”

Section: Putative Mechanism Of Dnaacos Suppressionmentioning

confidence: 99%

Overinitiation of chromosome replication in the Escherichia coli dnaAcos mutant depends on activation of oriC function by the dam gene product

Katayama

Akimitsu

Mizushima

et al. 1997

Molecular Microbiology

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Section: Putative Mechanism Of Dnaacos Suppressionmentioning

confidence: 99%

Overinitiation of chromosome replication in the Escherichia coli dnaAcos mutant depends on activation of oriC function by the dam gene product

Katayama

Akimitsu

Mizushima

et al. 1997

Molecular Microbiology

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“…One of the mechanisms that prevents reinitiation of already initiated origins in E. coli is sequestration (Campbell & Kleckner, 1990;Lu et al, 1994;von Freiesleben et al, 1994). This process functions by specific inactivation of newly replicated origins by the protein SeqA (Slater et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Replication patterns and organization of replication forks in Vibrio cholerae

2011

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We have investigated the replication patterns of the two chromosomes of the bacterium Vibrio cholerae grown in four different media. By combining flow cytometry and quantitative real-time PCR with computer simulations, we show that in rich media, V. cholerae cells grow with overlapping replication cycles of both the large chromosome (ChrI) and the small chromosome (ChrII). In Luria-Bertani (LB) medium, initiation occurs at four copies of the ChrI origin and two copies of the ChrII origin. Replication of ChrII was found to occur at the end of the ChrI replication period in all four growth conditions. Novel cell-sorting experiments with marker frequency analysis support these conclusions. Incubation with protein synthesis inhibitors indicated that the potential for initiation of replication of ChrII was present at the same time as that of ChrI, but was actively delayed until much of ChrI was replicated. Investigations of the localization of SeqA bound to new DNA at replication forks indicated that the forks were co-localized in pairs when cells grew without overlapping replication cycles and in higher-order structures during more rapid growth. The increased degree of fork organization during rapid growth may be a means by which correct segregation of daughter molecules is facilitated.

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“…The beststudied solitary methyltransferase in E. coli is DNA adenine methyltransferase, which targets the G m ATC motif 13 . This methyl mark has important roles in regulating on-off gene expression by influencing the binding properties of transcription factors to DNA sites containing the methylated motif 14,15 ; it also influences replication by regulating the recruitment of the initiator of replication to the origin of replication 16,17 and regulates DNA repair by informing the methyl-directed mismatch repair system about the age of the DNA strand [18][19][20] . Another well-studied adenine methyltransferase is CcrM in Caulobacter crescentus, which methylates the GANTC site and acts as a cell cycle regulator 21 .…”

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confidence: 99%

Genomics of DNA cytosine methylation in Escherichia coli reveals its role in stationary phase transcription

et al. 2012

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DnA cytosine methylation regulates gene expression in mammals. In bacteria, its role in gene expression and genome architecture is less understood. Here we perform high-throughput sequencing of bisulfite-treated genomic DnA from Escherichia coli K12 to describe, for the first time, the extent of cytosine methylation of bacterial DnA at single-base resolution. Whereas most target sites (C m CWGG) are fully methylated in stationary phase cells, many sites with an extended CC m CWGG motif are only partially methylated in exponentially growing cells. We speculate that these partially methylated sites may be selected, as these are slightly correlated with the risk of spontaneous, non-synonymous conversion of methylated cytosines to thymines. microarray analysis in a cytosine methylation-deficient mutant of E. coli shows increased expression of the stress response sigma factor Rpos and many of its targets in stationary phase. Thus, DnA cytosine methylation is a regulator of stationary phase gene expression in E. coli.

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E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork

Cited by 444 publications

References 47 publications

Overinitiation of chromosome replication in the Escherichia coli dnaAcos mutant depends on activation of oriC function by the dam gene product

Overinitiation of chromosome replication in the Escherichia coli dnaAcos mutant depends on activation of oriC function by the dam gene product

Replication patterns and organization of replication forks in Vibrio cholerae

Genomics of DNA cytosine methylation in Escherichia coli reveals its role in stationary phase transcription

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