E-selectin as a target for drug delivery and molecular imaging

Jubeli, Emile; Moine, Laurence; Vergnaud-Gauduchon, Juliette; Barratt, Gillian

doi:10.1016/j.jconrel.2011.09.084

Cited by 119 publications

(78 citation statements)

References 137 publications

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“…Vascular endothelial cell expression of E-selectin is entirely under transcriptional control, and cell surface expression can be detected approximately 2 hours after acute stimulation (29,31,32). In patients with IBD, both P-selectin and E-selectin have been shown to be constitutively expressed on inflamed vascular endothelium during the active phase of the disease (7,(33)(34)(35). Thus, both markers could be further evaluated as promising imaging biomarkers with In murine colitis models, it has been shown that FDG uptake was specifically correlated with glucose transporter 1 expression by CD4 + T cells, and those cells were identified as the major cellular source of FDG uptake in murine colitis (20).…”

Section: Discussionmentioning

confidence: 99%

Molecular Imaging of Inflammation in Inflammatory Bowel Disease with a Clinically Translatable Dual-Selectin–targeted US Contrast Agent: Comparison with FDG PET/CT in a Mouse Model

Machtaler¹,

Bettinger²,

Lutz³

et al. 2013

Radiology

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Purpose:To develop and test a molecular imaging approach that uses ultrasonography (US) and a clinically translatable dual-targeted (P-and E-selectin) contrast agent (MB Selectin ) in the quantification of inflammation at the molecular level and to quantitatively correlate selectin-targeted US with fluorodeoxyglucose (FDG) combined positron emission tomography (PET) and computed tomography (CT) in terms of visualization and quantification of different levels of inflammation in a murine acute colitis model. Materials and Methods:Animal studies were approved by the Institutional Admin- Results:MB Selectin showed strong attachment to both human and mouse P-and E-selectin compared with MB Control in vitro (P  .002). In vivo, US signal was significantly increased (P , .001) in mice with acute colitis (173.8 arbitrary units [au] 6 134.8 [standard deviation]) compared with control mice (5.0 au 6 4.5). US imaging signal strongly correlated with FDG uptake on PET/CT images (r = 0.89, P , .001). Ex vivo analysis enabled confirmation of inflammation in mice with acute colitis and high expression levels of P-and E-selectin in mucosal capillaries (P = .014). Conclusion:US with MB Selectin specifically enables detection and quantification of inflammation in a murine acute colitis model, leveraging the natural pathway of leukocyte recruitment in inflammatory tissue. US imaging with MB Selectin correlates well with FDG uptake at PET/CT imaging.

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Section: Discussionmentioning

confidence: 99%

Molecular Imaging of Inflammation in Inflammatory Bowel Disease with a Clinically Translatable Dual-Selectin–targeted US Contrast Agent: Comparison with FDG PET/CT in a Mouse Model

Machtaler¹,

Bettinger²,

Lutz³

et al. 2013

Radiology

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“…Another recent use of boroxole-saccharide interactions for the delivery of small molecules and proteins into cells was the delivery of a small enzyme into cells [17,33]. Often, large molecules are not taken up by the cell because they cannot get close enough due to the layer of charged saccharides surrounding it.…”

Section: Detection Of Gram-positive Bacteriamentioning

confidence: 99%

Molecular recognition with boronic acids—applications in chemical biology

2013

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Small molecules have long been used for the selective recognition of a wide range of analytes. The ability of these chemical receptors to recognise and bind to specific targets mimics certain biological processes (such as proteinsubstrate interactions) and has therefore attracted recent interest. Due to the abundance of biological molecules possessing polyhydroxy motifs, boronic acids-which form fivemembered boronate esters with diols-have become increasingly popular in the synthesis of small chemical receptors. Their targets include biological materials and natural products including phosphatidylinositol bisphosphate, saccharides and polysaccharides, nucleic acids, metal ions and the neurotransmitter dopamine. This review will focus on the many ways in which small chemical receptors based on boronic acids have been used as biochemical tools for various purposes, including sensing and detection of analytes, interference in signalling pathways, enzyme inhibition and cell delivery systems. The most recent developments in each area will be highlighted.

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“…Proof for the benefi t for drug developers is best exemplifi ed by the recent development of artifi cial exosomes. These are liposomes coated with MHC-peptide complexes and Fab regions against T cell receptors to mediate cell surface adhesion [63,64]. Presumably, many further experiments will be inspired by lessons of natural EVs that successfully overcame evolutional challenges.…”

Section: Discussionmentioning

confidence: 99%

Microencapsulation technology by nature: Cell derived extracellular vesicles with therapeutic potential

Kittel¹,

Falus²,

Buzás³

2013

European Journal of Microbiology and Immunology

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Cell derived extracellular vesicles are submicron structures surrounded by phospholipid bilayer and released by both prokaryotic and eukaryotic cells. The sizes of these vesicles roughly fall into the size ranges of microbes, and they represent efficient delivery platforms targeting complex molecular information to professional antigen presenting cells. Critical roles of these naturally formulated units of information have been described in many physiological and pathological processes. Extracellular vesicles are not only potential biomarkers and possible pathogenic factors in numerous diseases, but they are also considered as emerging therapeutic targets and therapeutic vehicles. Strikingly, current drug delivery systems, designed to convey therapeutic proteins and peptides (such as liposomes), show many similarities to extracellular vesicles. Here we review some aspects of therapeutic implementation of natural, cell-derived extracellular vesicles in human diseases. Exploration of molecular and functional details of extracellular vesicle release and action may provide important lessons for the design of future drug delivery systems.

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E-selectin as a target for drug delivery and molecular imaging

Cited by 119 publications

References 137 publications

Molecular Imaging of Inflammation in Inflammatory Bowel Disease with a Clinically Translatable Dual-Selectin–targeted US Contrast Agent: Comparison with FDG PET/CT in a Mouse Model

Molecular Imaging of Inflammation in Inflammatory Bowel Disease with a Clinically Translatable Dual-Selectin–targeted US Contrast Agent: Comparison with FDG PET/CT in a Mouse Model

Molecular recognition with boronic acids—applications in chemical biology

Microencapsulation technology by nature: Cell derived extracellular vesicles with therapeutic potential

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