2014
DOI: 10.1021/bm501403f
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E-Spun Composite Fibers of Collagen and Dragline Silk Protein: Fiber Mechanics, Biocompatibility, and Application in Stem Cell Differentiation

Abstract: Biocomposite matrices with high mechanical strength, high stability, and the ability to direct matrix-specific stem cell differentiation are essential for the reconstruction of lesioned tissues in tissue engineering and cell therapeutics. Toward this end, we used the electrospinning technique to fabricate well-aligned composite fibers from collagen and spider dragline silk protein, obtained from the milk of transgenic goats, mimicking the native extracellular matrix (ECM) on a similar scale. Collagen and the d… Show more

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Cited by 64 publications
(58 citation statements)
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“…16 The aligned fibers mimicked the locally oriented collagen fibers in native tissues. The incorporation of synthetic spider dragline silk proteins in collagen significantly enhanced the mechanical strength and stability of the E-spun fibers.…”
Section: Introductionmentioning
confidence: 93%
See 2 more Smart Citations
“…16 The aligned fibers mimicked the locally oriented collagen fibers in native tissues. The incorporation of synthetic spider dragline silk proteins in collagen significantly enhanced the mechanical strength and stability of the E-spun fibers.…”
Section: Introductionmentioning
confidence: 93%
“…16 The spinning conditions were optimized to obtain fibers with a desired dimension, density, and alignment. The parameters are summarized in Table S1.…”
Section: Experimental Sectionmentioning
confidence: 99%
See 1 more Smart Citation
“…A number of methods have been utilized to spin spider silks from dope solutions including electrospinning [37][38][39][40] , microfluidics 41 , and wet-spinning 9-12, 14, 15, 42-45 . Since wetspinning has been extensively employed on recombinant spidroins, alongside recombinant honeybee silk 46 , and regenerated Bombyx mori silkworm fibroin [47][48][49][50] , we focused on applying this technique to AcSp1.…”
Section: Introductionmentioning
confidence: 99%
“…Recombinant expression of spider silks is essential due to the territorial behavior of the natural hosts [120] and has been carried out in E. coli, [121] yeast, [122] silk worms, [123] and, in a scaledup production system, transgenic goats. [124] Silk-elastin-like PROGRESS REPORT polymers (SELPs), which contain repetitive amino acid sequence motifs derived from both silk fibroin and mammalian elastin, [125] have been produced recombinantly and at up to gram per liter yields in E. coli, [126] enabling their increasingly widespread investigation in structural [127] and controlled release/drug delivery applications. [127a,128] …”
Section: Expression Systems For Recombinant Productsmentioning
confidence: 99%