e0078 The effect of acute atorvastatin on cardioprotection of ischaemic postconditioning in diabetes mellitus

Abstract

Objective To evaluate the role of prenatal chronic hypoxia on cardiac function in adult rabbits offspring via echocardiography. Methods 16 New Zealand rabbits were divided randomly into two groups: prenatal chronic hypoxia group (12% O 2 , n¼8) and normal oxygen group (21% O 2 , n¼8). After delivery, two male offspring of each maternal rabbit were selected and breast-fed for 3 months. Then they were randomly divided into high-fat diet and normal diet respectively. Therefore, four groups were included: Prenatal Chronic Hypoxia with High Fat Diet (n¼8), Non-Prenatal Chronic Hypoxia with High Fat Diet (n¼8), Prenatal Chronic Hypoxia with Normal Diet (n¼8) and Normal Control (n¼8). At 6 months of age, the offspring rabbits were undergoing echocardiography examination for left ventricular (LV) dimensions, shortening fraction, ejection fraction and Tei index, and cardiocyte caspaseÀ3 activity detection. Results Prenatal chronic hypoxia induced a thickening of interventricular septum (main effect is 0.66 mm, p<0.01), decrease in ejection fraction of left ventricle (main effect is e4.84%, p<0.05), increase of Tei index (main effect is 0.08, p<0.01) and cardiocyte caspaseÀ3 activity (main effect is 0.47 unit, p<0.05) in 6-month-old prenatal chronic hypoxia offspring. All these effects were aggravated significantly when hyperlipaemia was imposed (p<0.05). Conclusions Echocardiography is a useful tool to evaluate the role of prenatal chronic hypoxia on cardiac function in adult rabbits offspring. Prenatal chronic hypoxia leads to cardiac dysfunction in adult rabbits offspring. This effect is aggravated by hyperlipoidemia. Objective GeneChip is one of the low-throughput gene screening tools, which is quite suitable for detecting target genes associated with certain pathophysiologic process. Our team has applied such technique into the studying of oxidative stress during the Acute Myocardial Infarction (AMI) and the following ischaemia/reperfusion injury (IRI) after PCI. Methods 11 patients with confirmed STEMI were admitted to our ER and CCU were involved into our study. 10 healthy volunteers with matching age and sex were set as the controlled group. Blood samples were collected immediately after the diagnosis and the same procedure was done on the 3 rd and 7 th day. Peripheral blood mononuclear cells (PBMCs) were extracted for RNA extraction. Human Stress & Toxicity Pathway Finder PCR Array was applied for corresponding gene screening. Real Time PCR was applied to confirm the candidate genes mRNA expression. Results 12 genes were detected with significant changes in the PBMCs of STEMI patients. GADD45A (associated with cell growth/ aging), PRDX2 (associated with oxidative stress), HSPD1, DNAJB1, DNAJB2 (associated with heat shock process), RAD50 (associated with DNA restoration), TNFSF6, TRADD (associated with apoptosis) displayed up-regulated expression. CCNG1 (associated with cell proliferation/cancer), CAT, CYP1A1 (associated with oxidative stress), ATM (associated with DNA restoration) were down-regulated. Further RT-...