E2-CD154 vaccine candidate is safe and immunogenic in pregnant sows, and the maternal derived neutralizing antibodies protect piglets from classical swine fever virus challenge

Pérez-Pérez, Danny; Sordo-Puga, Yusmel; Rodríguez-Moltó, María Pilar; Sardina, Talía; Santana, Elaine; Montero, Carlos; Ancízar, Julio; Cabrera, Yaneris; Tuero, Ángela; Naranjo, Paula; Sosa-Testé, Iliana; Fernandez, F.; Valdés, Rodolfo; Duarte, Carlos A.; Suárez-Pedroso, Marisela

doi:10.1016/j.vetmic.2021.109153

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…The safety and effectiveness of Porvac® in pigs from different categories has been widely documented [19][20][21][22]. This vaccine has also been capable of providing a very rapid onset of protection [11] and to protect against vertical transmission [12].…”

Section: Discussionmentioning

confidence: 99%

Stability of closed and needle-punctured vials of Porvac ® subunit vaccine against classical swine fever subjected to thermal stress

Sardina-González,

Vargas-Hernández,

Sordo-Puga

et al. 2024

Preprint

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Background Classical Swine Fever (CSF) is still one of the most economically important viral diseases of pigs. In endemic countries, the disease is controlled mostly through vaccination, hence, the availability of safe and effective vaccines is of utmost importance. Vaccines intended for application in developing countries must also be thermally stable, since the infrastructure needed to maintain a cold chain in those countries is usually lacking. Porvac® is a second-generation subunit marker vaccine against CSF that has demonstrates to be safe and protective. Previous studies have also shown that the vaccine is stable for 1 week at 37 oC and have a shelf life of at least 36 months at 2–8 oC. The aim of this work was to further explore the accelerated stability of Porvac® by assessing the physicochemical properties of the emulsion, and the safety and efficacy of the vaccine subjected to more drastic conditions of thermal stress: (1) 25 oC for 12 months; (2) 30oC and 37 oC for one month and (3) 15 days at 37°C after the cap of the vials had been needle-punctured. Results The vaccine subjected to all these conditions did not show significant changes in the physicochemical properties of the emulsion; did not produce local or systemic adverse reactions in pigs, and the chromatographic profile of the recovered antigen was preserved. All vaccinated swine developed protective neutralizing antibody titers ≥ 1:1000 at 28 days post vaccination. Conclusions Porvac® is stable in all the experimental conditions tested, even after cap puncture, and retains the capacity to induce protective neutralizing antibodies. These results reinforce the robustness of the vaccine, and support its use as a very attractive alternative to modified live vaccines in developing countries endemic for CSF.

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Section: Discussionmentioning

confidence: 99%

Stability of closed and needle-punctured vials of Porvac ® subunit vaccine against classical swine fever subjected to thermal stress

Sardina-González,

Vargas-Hernández,

Sordo-Puga

et al. 2024

Preprint

Self Cite

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“…However, its safety and immunogenicity in pregnant sows and the ability of maternally sourced neutralizing antibodies (MDNA) to protect offspring have not been demonstrated. Perez et al, evaluated the safety and immunogenicity of E2-CD154 in gestating sows and the ability of MDNA to protect progeny [106]. To introduce the E2-CD154 vaccine into areas where MLV vaccination is available, a further study was conducted in which gestating sows were vaccinated with the candidate vaccine and high maternally derived antibody (MDA) titers were present in piglets born to sows vaccinated with E2-CD154.…”

Section: Adjuvantmentioning

confidence: 99%

“…Complete protection against classical swine fever virus at 7 dpi, preventing vertical transmission, and the CD154 molecule enhances cellular immunity [105][106][107] Subunit vaccine E2-IFN-γ E rns…”

Section: Subunit Vaccine E2-cd154 E Rnsmentioning

confidence: 99%

The Development of Classical Swine Fever Marker Vaccines in Recent Years

Niu

et al. 2022

Vaccines

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Classical swine fever (CSF) is a severe disease that has caused serious economic losses for the global pig industry and is widely prevalent worldwide. In recent decades, CSF has been effectively controlled through compulsory vaccination with a live CSF vaccine (C strain). It has been successfully eradicated in some countries or regions. However, the re-emergence of CSF in Japan and Romania, where it had been eradicated, has brought increased attention to the disease. Because the traditional C-strain vaccine cannot distinguish between vaccinated and infected animals (DIVA), this makes it difficult to fight CSF. The emergence of marker vaccines is considered to be an effective strategy for the decontamination of CSF. This paper summarizes the progress of the new CSF marker vaccine and provides a detailed overview of the vaccine design ideas and immunization effects. It also provides a methodology for the development of a new generation of vaccines for CSF and vaccine development for other significant epidemics.

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Stability of closed and needle-punctured vials of Porvac® subunit vaccine against classical swine fever subjected to thermal stress

Sardina-González,

Vargas-Hernández,

Sordo-Puga

et al. 2024

BMC Vet Res

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E2-CD154 vaccine candidate is safe and immunogenic in pregnant sows, and the maternal derived neutralizing antibodies protect piglets from classical swine fever virus challenge

Cited by 3 publications

References 27 publications

Stability of closed and needle-punctured vials of Porvac ® subunit vaccine against classical swine fever subjected to thermal stress

Stability of closed and needle-punctured vials of Porvac ® subunit vaccine against classical swine fever subjected to thermal stress

The Development of Classical Swine Fever Marker Vaccines in Recent Years

Stability of closed and needle-punctured vials of Porvac® subunit vaccine against classical swine fever subjected to thermal stress

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