EAF2 Suppresses Hypoxia-Induced Factor 1α Transcriptional Activity by Disrupting Its Interaction with Coactivator CBP/p300

Chen, Zhu; Liu, Xing; Mei, Zhichao; Wang, Zhou; Xiao, Wuhan

doi:10.1128/mcb.00718-13

Cited by 38 publications

(50 citation statements)

References 62 publications

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“…Western blot analysis and co-immunoprecipitation, the experimental procedures have been described previously (31). Anti-HA antibody and anti-FLAG antibody-conjugated agarose beads were purchased from Sigma-Aldrich.…”

Section: Co-immunoprecipitation and Western Blot Analysis-formentioning

confidence: 99%

FBXO32 Targets c-Myc for Proteasomal Degradation and Inhibits c-Myc Activity

Mei

Zhang

et al. 2015

Journal of Biological Chemistry

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Section: Co-immunoprecipitation and Western Blot Analysis-formentioning

confidence: 99%

FBXO32 Targets c-Myc for Proteasomal Degradation and Inhibits c-Myc Activity

Mei

Zhang

et al. 2015

Journal of Biological Chemistry

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“…SHARP1 promotes HIF-α degradation by serving as the HIF-presenting factor to the proteasome [26]. EAF2 is a specific HIF-1α downstream target, which interacts with HIF-1α but not HIF-2α, resulting in the inhibition of HIF-1α transcriptional activity via preventing p300 from binding to HIF-1α [27]. Other proteins do not interact with HIF-α directly, but interact with other factors that regulate HIF-α, e.g., pVHL and PHDs among others.…”

Section: Hif and The Regulation Of The Hypoxia Signaling Pathwaymentioning

confidence: 99%

The hypoxia signaling pathway and hypoxic adaptation in fishes

Xiao

2015

Sci. China Life Sci.

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141

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The hypoxia signaling pathway is an evolutionarily conserved cellular signaling pathway present in animals ranging from Caenorhabditis elegans to mammals. The pathway is crucial for oxygen homeostasis maintenance. Hypoxia-inducible factors (HIF-1α and HIF-2α) are master regulators in the hypoxia signaling pathway. Oxygen concentrations vary a lot in the aquatic environment. To deal with this, fishes have adapted and developed varying strategies for living in hypoxic conditions. Investigations into the strategies and mechanisms of hypoxia adaptation in fishes will allow us to understand fish speciation and breed hypoxia-tolerant fish species/strains. This review summarizes the process of the hypoxia signaling pathway and its regulation, as well as the mechanism of hypoxia adaptation in fishes. Approximately 2.5 billion years ago, photosynthesis led to the accumulation of oxygen to levels that were likely toxic to many obligate anaerobes. However, increased availability of atmospheric O 2 led to the evolution of an extraordinarily efficient system of oxidative phosphorylation. In this system, chemical energy stored in the carbon bonds of organic molecules is transferred to the high-energy phosphate bond in ATP, which is then used to power physicochemical reactions in living cells [1]. Additionally, O 2 serves as the final electron acceptor in oxidative phosphorylation, which is not only required for energy production, but is also the direct substrate of many enzymes. Thus, it is critical for the growth, development, and reproduction of organisms. Consequently, metazoans have evolved complicated systems of cellular metabolism and physiology to maintain oxygen homeostasis and have developed a biochemical response to low oxygen levels [2]. There are a number of oxygen-sensing pathways that promote hypoxia tolerance by activating transcription and inhibiting translation: the energy and nutrient sensor mTOR, the unfolded protein response that activates the endoplasmic stress response, and the nuclear factor (NF)-B transcriptional response [3]. However, hypoxia-inducible factors (HIFs) are recognized as master regulators of the cellular response to hypoxic stress [4,5]. The hypoxia signaling pathway is evolutionarily conserved from Caenorhabditis elegans to human beings and it activates similar or homogenous gene expression, resulting in similar physical and biochemical responses. Compared with the terrestrial environment, oxygen concentrations vary greatly in the aquatic environment [6]. Thus, compared with most birds and mammals, fishes are tolerant of this varying oxygen availability. Natural selection by oxygen concentration has facilitated the evolution of fishes with a range of adaptations to variable oxygen concentration. Even in waters at the same latitude, closely related species or different strains within a species exhibit varied adaptations to oxygen concentration. Additionally, closely related fishes distributed in waters at different latitudes exhibit extensive variation in their tolerance of hypoxia. De...

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“…The immunoprecipitate and the total cell lysate were boiled with 13 SDS sample loading buffer, separated on SDS-PAGE, and transferred to a polyvinylidene difluoride membrane (Millipore). Western blot analysis was performed as described previously (39). Anti-Myc Ab-conjugated agarose beads were purchased from Sigma-Aldrich.…”

Section: Immunoprecipitation and Western Blottingmentioning

confidence: 99%

Zebrafish foxo3b Negatively Regulates Antiviral Response through Suppressing the Transactivity of irf3 and irf7

Xing

Cai

Zhang

et al. 2016

The Journal of Immunology

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Forkhead box O (FOXO)3, a member of the FOXO family of transcription factors, plays key roles in various cellular processes, including development, longevity, reproduction, and metabolism. Recently, FOXO3 has also been shown to be involved in modulating the immune response. However, how FOXO3 regulates immunity and the underlying mechanisms are still largely unknown. In this study, we show that zebrafish (Danio rerio) foxo3b, an ortholog of mammalian FOXO3, is induced by polyinosinic-polycytidylic acid stimulation and spring viremia of carp virus (SVCV) infection. We found that foxo3b interacted with irf3 and irf7 to inhibit ifr3/irf7 transcriptional activity, thus resulting in suppression of SVCV or polyinosinic-polycytidylic acid–induced IFN activation. By suppressing expression of key antiviral genes, foxo3b negatively regulated the cellular antiviral response. Furthermore, upon SVCV infection, the expression of the key antiviral genes was significantly enhanced in foxo3b-null zebrafish larvae compared with wild-type larvae. Additionally, the replication of SVCV was inhibited in foxo3b-null zebrafish larvae, leading to a higher survival rate. Our findings suggest that by suppressing irf3/irf7 activity, zebrafish foxo3b negatively regulates the antiviral response, implicating the vital role of the FOXO gene family in innate immunity.

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EAF2 Suppresses Hypoxia-Induced Factor 1α Transcriptional Activity by Disrupting Its Interaction with Coactivator CBP/p300

Cited by 38 publications

References 62 publications

FBXO32 Targets c-Myc for Proteasomal Degradation and Inhibits c-Myc Activity

FBXO32 Targets c-Myc for Proteasomal Degradation and Inhibits c-Myc Activity

The hypoxia signaling pathway and hypoxic adaptation in fishes

Zebrafish foxo3b Negatively Regulates Antiviral Response through Suppressing the Transactivity of irf3 and irf7

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