“…According to Keefe and Levi (1996), the maturation of the external and middle ear in infants aged from 0 to 6 months has significant effects on the interpretation of measured ear canal responses to acoustic stimuli. The developmental changes include a decrease in the mass of the middle ear from mesenchyme loss; changes in bone density; ossicular joint tightening; increases in the size of the external auditory canal, middle-ear cavity and mastoid; a lesser union between the stapes and the annular ligament; the fusing of the tympanic ring; a change in tympanic membrane orientation and flexibility; and, the formation of the bony portion of the ear canal wall (Eby & Nadol, 1986;Holte et al, 1991;Jaffe et al, 1970;Keefe et al, 1993;Keith, 1975;McLellan & Webb, 1957;Saunders et al, 1983). Given these developmental effects, tympanometric normative data at birth may not be identical to that obtained at a later date, such as 6Á7 weeks when a comprehensive audiological assessment is likely to be scheduled for neonates who fail their initial newborn screening.…”