Early Acquisition of Neural Crest Competence During hESCs Neuralization

Curchoe, Carol Lynn; Maurer, Jochen; McKeown, Sonja J.; Cattarossi, Giulio; Cimadamore, Flavio; Nilbratt, Mats; Snyder, Evan Y.; Bronner‐Fraser, Marianne; Terskikh, Alexey V.

doi:10.1371/journal.pone.0013890

Cited by 73 publications

(79 citation statements)

References 80 publications

(109 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The migrated cells from rosettes in the present study showed similar expression of MSC markers, as was observed in other studies (2,34,58). However, while a previous report showed that human ES cell-derived NCSCs do not express CD73 (31), our migrated cells exhibited higher levels of CD73 expression, in consistent with previous reports (8,58). Further analyses are required to examine the differences between iPS cell-derived and ES cell-derived NCCs in terms of CD73 expression.…”

Section: Discussionsupporting

confidence: 92%

Induction of neural crest cells from human dental pulp-derived induced pluripotent stem cells

et al. 2017

View full text Add to dashboard Cite

We previously generated induced pluripotent stem (iPS) cells from human dental pulp cells of deciduous teeth. Neural crest cells (NCCs) play a vital role in the development of the oral and maxillofacial region. Therefore, NCCs represent a cell source for bone, cartilage, and tooth-related tissue engineering. In this study, we examined whether iPS cells are capable of differentiating into NCCs through modification of the human embryonic stem cell protocol. First, iPS cells were dissociated into single cells and then reaggregated in low-cell-adhesion plates with neural induction medium for 8 days in suspension culture to form neurospheres. The neurospheres were transferred to fibronectin-coated dishes and formed rosette structures. The migrated cells from the rosettes abundantly expressed NCC markers, as evidenced by real-time polymerase chain reaction, immunofluorescence, and flow cytometric analysis. Furthermore, the migrated cells exhibited the ability to differentiate into neural crest lineage cells in vitro. They also exhibited tissue-forming potential in vivo, differentiating into bone and cartilage. Collectively, the migrated cells had similar characteristics to those of NCCs. These results suggest that human dental pulp cell-derived iPS cells are capable of differentiating into NCCs. Therefore, iPS cell-derived NCCs represent cell sources for bone and cartilage tissue engineering.

show abstract

Section: Discussionsupporting

confidence: 92%

Induction of neural crest cells from human dental pulp-derived induced pluripotent stem cells

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Our previous microarray gene expression analysis of hESC-derived multipotent NPCs (11,30,31) suggested that SOX2 regulates the expression of LIN28, a well-studied repressor of let-7 family biosynthesis (13-16). Immunofluorescence analysis confirmed that SOX2 and LIN28 were coexpressed in all NPCs (Fig.…”

Section: Sox2mentioning

confidence: 99%

SOX2–LIN28/let-7 pathway regulates proliferation and neurogenesis in neural precursors

Cimadamore

Amador-Arjona

Chen

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

202

182

View full text Add to dashboard Cite

The transcription factor SRY (sex-determining region)-box 2 (SOX2) is an important functional marker of neural precursor cells (NPCs) and plays a critical role in self-renewal and neuronal differentiation; however, the molecular mechanisms underlying its functions are poorly understood. Using human embryonic stem cellderived NPCs to model neurogenesis, we found that SOX2 is required to maintain optimal levels of LIN28, a well-characterized suppressor of let-7 microRNA biogenesis. Exogenous LIN28 expression rescued the NPC proliferation deficit, as well as the early but not the late stages of the neurogenic deficit associated with the loss of SOX2. We found that SOX2 binds to a proximal site in the LIN28 promoter region and regulates LIN28 promoter acetylation, likely through interactions with the histone acetyltransferase complex. Misexpression of let-7 microRNAs in NPCs reduced proliferation and inhibited neuronal differentiation, phenocopying the loss of SOX2. In particular, we identified let-7i as a novel and potent inhibitor of neuronal differentiation that targets MASH1 and NGN1, two well-characterized proneural genes. In conclusion, we discovered the SOX2-LIN28/let-7 pathway as a unique molecular mechanism governing NPC proliferation and neurogenic potential.neural stem cells | mechanisms of pluripotency S elf-renewing, multipotent neural precursor cells (NPCs) are capable of terminally differentiating into neuronal and glial lineages during development and in the adult nervous system (1, 2). Disruption of the pathways controlling NPC biology has been implicated in various pathologies, including autism (3), Treacher Collins syndrome (4), and neural tube defects (5), emphasizing the importance of gaining a better understanding of the underlying molecular events and how they may be manipulated to treat and prevent such pathologies. The HMG-box transcription factor SOX2 is ubiquitously expressed in NPCs and supports their self-renewal (6). SOX2 is also required for neurogenesis in the central nervous system (7-10). Recently, we used human embryonic stem cells (hESCs) and mouse models to demonstrate a critical requirement for SOX2 for sensory neurogenesis in dorsal root ganglia (11). However, the mechanisms by which SOX2 functions in self-renewal and neuronal differentiation remain poorly understood.Small, noncoding microRNAs (miRNAs) are transcribed as long precursors (pri-miRNAs) that are sequentially processed by the RNases Drosha/Pasha to form pri-miRNAs and by Dicer to form the mature miRNAs of ∼20-25 nucleotides. The miRNAs function through imperfect base-pairing with hundreds of target mRNAs to trigger their degradation (or block their translation) by the RNA-induced silencing complex, RISC (12). In some cases, miRNA maturation is tightly controlled; for example, the RNA-binding protein LIN28 regulates the biogenesis of the let-7 miRNA family by inhibiting their maturation at both the primiRNA (13, 14) and premiRNA (15, 16) processing steps. Intriguingly, LIN28 protein was found to be associated with SO...

show abstract

“…These cells express the neural crest specifier SOX10 and clonal analysis indicates they are multipotent. Importantly, when these cells are injected in chick embryos, they migrate normally and contribute to the same derivatives as endogenous neural crest cells (Curchoe et al 2010).…”

Section: Neural Crest Cells and Diseasementioning

confidence: 99%

Insights into neural crest development and evolution from genomic analysis

2013

View full text Add to dashboard Cite

The neural crest is an excellent model system for the study of cell type diversification during embryonic development due to its multipotency, motility, and ability to form a broad array of derivatives ranging from neurons and glia, to cartilage, bone, and melanocytes. As a uniquely vertebrate cell population, it also offers important clues regarding vertebrate origins. In the past 30 yr, introduction of recombinant DNA technology has facilitated the dissection of the genetic program controlling neural crest development and has provided important insights into gene regulatory mechanisms underlying cell migration and differentiation. More recently, new genomic approaches have provided a platform and tools that are changing the depth and breadth of our understanding of neural crest development at a “systems” level. Such advances provide an insightful view of the regulatory landscape of neural crest cells and offer a new perspective on developmental as well as stem cell and cancer biology.

show abstract

Early Acquisition of Neural Crest Competence During hESCs Neuralization

Cited by 73 publications

References 80 publications

<b>Induction of neural crest cells from human dental pulp-derived induced pluripotent stem </b><b>cells </b>

<b>Induction of neural crest cells from human dental pulp-derived induced pluripotent stem </b><b>cells </b>

SOX2–LIN28/let-7 pathway regulates proliferation and neurogenesis in neural precursors

Insights into neural crest development and evolution from genomic analysis

Contact Info

Product

Resources

About