Early and chronic gray matter volume changes in limbic encephalitis revealed by voxel‐based morphometry

Abstract

SUMMARYObjective: Antibody-associated limbic encephalitis (LE) is an increasingly recognized cause of mostly adult-onset temporal lobe epilepsy. Magnetic resonance imaging (MRI) typically shows volume and signal changes of the mesiotemporal structures. However, recent studies indicate that imaging characteristics depend on the type of the associated antibody. The aim of the present study was to investigate early and chronic gray matter (GM) volume changes in LE by means of voxel-based morphometry (VBM). Methods: Optimized VBM analysis was applied to altogether 73 MRI volumes of 55 patients with antibody-associated LE. Based on the time point of MRI acquisition, patients were split into two separate groups to enable the evaluation of early (≤2 years after LE onset) and chronic (>2 years after LE onset) GM volume changes. In addition, separate analyses for the two most common LE subtypes in our study cohort, that is, glutamic acid decarboxylase (GAD)-associated LE and voltage-gated potassium channel (VGKC)-complex-associated LE were performed. Age-and gender-matched healthy subjects served as controls. Results: Referring to the entire LE group, VBM revealed bi-amygdalar GM volume increase in the early disease stage. In the chronic disease stage, amygdala enlargement had resolved and we found GM volume reduction in the right cerebellar hemisphere. In the subgroup analysis, VBM showed corresponding bi-amygdalar GM volume increase in VGKC-complex-associated LE on early MRI, whereas no changes were found in GAD-associated LE. In the chronic disease stage, VBM revealed left frontal GM volume increase in VGKC-complex-associated LE and right frontoparietal GM volume reduction in GAD-associated LE. Significance: The present study provides further evidence of a predominant affection of the amygdala in the early disease stage of LE, which resolves during the later course of the disease. Furthermore, our results show that LE features distinct imaging characteristics depending on the associated antibody and thus may contribute to a better pathophysiologic understanding of this disease.

Focal CA3 hippocampal subfield atrophy following LGI1 VGKC-complex antibody limbic encephalitis

“…This result aligns with other recent studies that have conducted comparable voxel-based morphometry analyses (Wagner et al , 2015; Finke et al , 2017). …”

Mind-wandering in people with hippocampal damage

“…In line with previous reports of this patient population (Dalmau and Rosenfeld, 2014;Miller et al, 2017), manual (blinded) segmentation of the hippocampi from high-resolution structural MRI scans confirmed that our patients showed volume loss confined to the left (Patients -HPC: 2506mm 3 (mean) +/-394 (standard deviation), control participants -CTL: 3173 mm 3 +/-339, W=4.0, p=0.002) and right (HPC: 2678mm 3 +/-528, CTL: 3286mm 3 +/-301, W=8.0, p=0.01) hippocampus. To rule out pathological differences between patients and controls elsewhere in the brain, an automated voxel-based-morphometry (VBM; Ashburner, 2009) analysis was carried out on whole brain T1 weighted MRI images and, in line with previous reports on patients of this sort (Wagner et al, 2015;Finke et al, 2017;Miller et al, 2017), did not result in any significant group differences outside of the hippocampus even at a liberal uncorrected p-value of less than 0.001. Neuropsychologically, the patients displayed an impairment in immediate and delayed recall on the Logical Memory (short stories) test (Wechsler, 1997), and they recollected significantly fewer episodic ('internal'), but not semantic ('external') details on the Autobiographical Interview , as detailed in Table 2.…”

Distinct white matter integrity in glutamic acid decarboxylase and voltage‐gated potassium channel‐complex antibody‐associated limbic encephalitis

“…Volume and signal changes of mesiotemporal structures are well‐known characteristics of LE on magnetic resonance imaging (MRI) and have been demonstrated by both conventional and postprocessing imaging studies . In contrast to these well‐known changes of the gray matter, the white matter usually does not show any abnormalities on routine clinical MRI examinations.…”