Early aspects of gonadal sex differentiation in <i>Xenopus tropicalis</i> with reference to an antero‐posterior gradient

Jamil, Anwar El; Magre, Solange; Mazabraud, A; Penrad-Mobayed, May

doi:10.1002/jez.439

Cited by 33 publications

(23 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, in both anuran and urodele amphibians, the ovary is an ovisac [Dumond et al, 2008a;Piprek et al, 2012]. In S. tropicalis , ovary differentiation seems to occur along an antero-posterior gradient [El Jamil et al, 2008]. The different steps of ovarian differentiation in anurans have been described in detail in a comparison of 12 species [Ogielska and Kotusz, 2004].…”

Section: Gonad Differentiationmentioning

confidence: 99%

See 1 more Smart Citation

Sex Reversal in Amphibians

Flament¹

2016

Sex Dev

View full text Add to dashboard Cite

Amphibians have been widely used to study developmental biology due to the fact that embryo development takes place independently of the maternal organism and that observations and experimental approaches are easy. Some amphibians like Xenopus became model organisms in this field. In the first part of this article, the differentiation of the gonads in amphibians and the mechanisms governing this process are reviewed. In the second part, the state of the art about sex reversal, which can be induced by steroid hormones in general and by temperature in some species, is presented. Also information about pollutants found in the environment that could interfere with the development of the amphibian reproductive apparatus or with their reproductive physiology is given. Such compounds could play a part in the amphibian decline, since in the wild, many amphibians are endangered species.

show abstract

Section: Gonad Differentiationmentioning

confidence: 99%

“…1 ). In Silurana (Xenopus) tropicalis , testis differentiation seems to occur along an antero-posterior gradient [El Jamil et al, 2008]. Meiosis entry is not observed until after metamorphosis [Wallacides et al, 2009].…”

Section: Gonad Differentiationmentioning

confidence: 99%

Sex Reversal in Amphibians

Flament¹

2016

Sex Dev

View full text Add to dashboard Cite

show abstract

“…Compound (Sakura Finetechnical, Tokyo, Japan) by immersion in isopentane chilled with liquid nitrogen and stored at À801C until use. Immunostaining was performed according to Jamil et al (2008). Frozen sections were cut to 10 mm thickness with a Leitz digital cryostat (model 1720; Ernst Leitz, Midland, Ont., Canada) and incubated overnight at 41C with rabbit anti-Vasa antibody at the dilution 1/1,000.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Isolation and characterization of Vasa in the frog Rana rugosa

et al. 2010

View full text Add to dashboard Cite

We cloned a cDNA encoding Vasa, a member of the DEAD (Asp-Glu-Ala-Asp) family of proteins, from the ovary of the frog Rana rugosa. Comparative alignment of amino acid sequences with known Vasa from several species of vertebrate showed that the R. rugosa orthologue shares eight conserved regions with Vasa from other vertebrates. Vasa gene expression was restricted to the testis and ovary among ten different tissues examined. Vasa expression showed no sexual dimorphism during sex determination in R. rugosa, but became higher in the ovary thereafter. By Western blot analysis, a single Vasa band with a molecular weight of 80.9 kDa was detected. The same antibody immunohistochemically detected Vasa in a few cells in the embryonic endoderm at stage 15; the beginning of closure of neural folds, and in the cytoplasm of spermatogonia in the testis, and oocytes in the ovary of tadpoles at stage XX; marked by one or both forelegs protruded. Together, these results suggest that Vasa is a highly specific marker of germ cells and hence useful for studies of germ cell specification and function in amphibians as it already is in other species of both invertebrates and vertebrates such as Drosophila and zebrafish.

show abstract

“…In other nonhylid taxa, spermatids are found in testes later in ontogeny, such as in postmetamorphic juvenile stages (Lamotte et al, 1973;Chavadej et al, 2000;Gramapurohit et al, 2000;El Jamil et al, 2008;Fig. 8).…”

Section: Gonad Differentiationmentioning

confidence: 99%

The ontogeny of Pseudis platensis (Anura, Hylidae): Heterochrony and the effects of larval development on postmetamorphic life

Fabrezi

Quinzio

Goldberg

2009

Journal of Morphology

View full text Add to dashboard Cite

Recent studies have described the giant tadpole, delayed metamorphic transformations, and absence of postmetamorphic growth of the skeleton of Pseudis Platensis. These features address questions about derived patterns of life cycles and the role of the heterochrony during the metamorphosis in anurans. Using anatomical methods, we provide new data on the development of reproductive, digestive and integument systems, and age inference obtained from ontogenetic series of Pseudis platensis. Our results indicate that at the end of metamorphosis, the adult skin is completely differentiated, including the calcified dermal layer; the testis has seminiferous tubules with spermatogonia, spermatocytes, and spermatids; ovarian sacs present previtellogenic ova; and the adult digestive tract is fully formed. The froglets differ from adults only in being unable to reproduce. The entire life cycle of P. platensis can occur in 4 years. In the first year, larval development, growth to adult size, and gonad differentiation are completed. Long larval development rather than size of the tadpoles seems to be involved in the absence of juvenile stages.

show abstract

Early aspects of gonadal sex differentiation in Xenopus tropicalis with reference to an antero‐posterior gradient

Cited by 33 publications

References 49 publications

Sex Reversal in Amphibians

Sex Reversal in Amphibians

Isolation and characterization of Vasa in the frog Rana rugosa

The ontogeny of Pseudis platensis (Anura, Hylidae): Heterochrony and the effects of larval development on postmetamorphic life

Contact Info

Product

Resources

About