Early bronchiolar damage following paraquat poisoning in mice

Etherton, J. E.; Gresham, G. A.

doi:10.1002/path.1711280105

Cited by 20 publications

(6 citation statements)

References 25 publications

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“…This diamine also causes extensive changes to Clara cells in vivo (18) and has a high toxicity rating (TD50 = 4 I.M) from the present in vitro studies.…”

Section: Effects Of Bronchiolar Toxins On the Plating Efficiency Of Cmentioning

confidence: 66%

“…Acrolein, a degradation product ofcyclophosphamide (16), is also moderately toxic (TD50 = 100 MM) in the in vitro system. Another drug, busulfan, has little effect on Clara cell attachment efficiency at doses < 100 AM, a finding that is similar to that with alloxan, an agent considered to selectively damage lung endothelial cells (18).…”

Section: Effects Of Bronchiolar Toxins On the Plating Efficiency Of Cmentioning

confidence: 76%

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Clara Cell Cultures from the Mouse and Their Reaction to Bronchiolar Toxins

Richards¹,

Oreffo²,

Lewis³

1990

Environmental Health Perspectives

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The major aim of this study was to determine if small numbers of freshly isolated mouse Clara cells could be used to rapidly screen the toxic effects of a number of diverse pulmonary toxins. A short-term (20 hr) culture of functionally competent (nitotetrazolium reductase positive) Clara cells was developed. In this culture the Clara cells were allowed to attach to an extracellular matrix in 96-well multiwell plates containing a culture medium of DCCM 1 and Ultroser G (0.4%). Pulmonary toxins (a total of 26 agents with concentrations ranging from 10 -7 M to 10 -3 M) were examined for their ability to reduce the attachment efficiency of functionally competent Clara cells and TD50 values (the amount of toxin required to reduce normal attachment efficiency by 50 %) were calculated. With the possible exception of some halogenated hydrocarbons, the simple toxicity test in vitro correlated well with the known effects of the bronchiolar necrotic agents in vivo. For 13 compounds studied there was a direct correlation between TD50 values in vitro and LD50 values (mostly oral) in rodents in vivo, the correlation coefficient of the regression line being 0.783.

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“…This diamine also causes extensive changes to Clara cells in vivo (18) and has a high toxicity rating (TD50 = 4 I.M) from the present in vitro studies.…”

Section: Effects Of Bronchiolar Toxins On the Plating Efficiency Of Cmentioning

confidence: 66%

Section: Effects Of Bronchiolar Toxins On the Plating Efficiency Of Cmentioning

confidence: 76%

Clara Cell Cultures from the Mouse and Their Reaction to Bronchiolar Toxins

Richards¹,

Oreffo²,

Lewis³

1990

Environmental Health Perspectives

View full text Add to dashboard Cite

show abstract

Section: Effects Of Bronchiolar Toxins On the Plating Efficiency Of Cmentioning

confidence: 76%

Clara cell cultures from the mouse and their reaction to bronchiolar toxins.

Richards

Oreffo

Lewis

1990

Environ Health Perspect

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The major aim of this study was to determine if small numbers of freshly isolated mouse Clara cells could be used to rapidly screen the toxic effects of a number of diverse pulmonary toxins. A short-term (20 hr) culture of functionally competent (nitotetrazolium reductase positive) Clara cells was developed. In this culture the Clara cells were allowed to attach to an extracellular matrix in 96-well multiwell plates containing a culture medium of DCCM 1 and Ultroser G (0.4%). Pulmonary toxins (a total of 26 agents with concentrations ranging from 10(-7) M to 10(-3) M) were examined for their ability to reduce the attachment efficiency of functionally competent Clara cells and TD50 values (the amount of toxin required to reduce normal attachment efficiency by 50%) were calculated. With the possible exception of some halogenated hydrocarbons, the simple toxicity test in vitro correlated well with the known effects of the bronchiolar necrotic agents in vivo. For 13 compounds studied there was a direct correlation between TD50 values in vitro and LD50 values (mostly oral) in rodents in vivo, the correlation coefficient of the regression line being 0.783.

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“…It is one of the most toxic poisons involved in suicide attempts. Paraquat primarily causes lung injury in mammals, where paraquat is accumulated through a process of active transport in Clara cells and alveolar type I and II epithelial cells [3][4][5][6][7]. There are two distinct phases in the development of pulmonary fibrosis by paraquat: an early destructive phase characterized by alveolitis, pulmonary edema, and infiltration of inflammatory cells, followed by a final fibrotic phase where fibroblasts proliferate, collagen is deposited, and pulmonary fibrosis occurs [3,[8][9][10].…”

Section: Introductionmentioning

confidence: 97%