Early Changes in Lung Tissue Hyaluronan (Hyaluronic Acid) and Hyaluronidase in Bleomycin-induced Alveolitis in Hamsters

Bray, Bonnie Anderson; Sampson, Phyllis; Osman, Mohamed; Giandomenico, Albert R.; Turino, Gerard M.

doi:10.1164/ajrccm/143.2.284

Cited by 47 publications

(22 citation statements)

References 13 publications

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“…These findings are consistent with studies demonstrating increased hyaluronan levels in the bronchoalveolar lavage fluid of patients with sarcoidosis [16], EAA [17], ARDS [18], IPF [19] and experimental bleomycin-induced alveolitis [20]. The synthesis and deposition of hyaluronan in experimental bleomycin-induced fibrosis is an early event which preceeds collagen deposition [20] and is related to the early inflammatory response following lung injury [21]. Hyaluronan is thought to play a role in regulation of fluid balance within the interstitium [22,23] which in inflammatory conditions may lead to an open oedematous matrix which facilitates cell migration, Cell migration may be faciltated by the binding of specific cell surface receptors to hyaluronan [24] as occurs in healing wounds [24].…”

Section: Discussionsupporting

confidence: 89%

Proteoglycans in granulomatous lung diseases

Bensadoun¹,

Burke²,

Hogg³

et al. 1997

Eur Respir J

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In nongranulomatous fibrotic lung conditions, we have recently shown that early collagen synthesis by myofibroblasts occurs in an extracellular matrix rich in the proteoglycan versican. We hypothesized that versican is associated with the process of collagen synthesis resulting from chronic inflammation. In this study, we examined the localization of proteoglycans and collagen in the granulomatous lung conditions, sarcoidosis, extrinsic allergic alveolitis (EAA) and tuberculosis (TB). Tissue from individuals with sarcoidosis (n=6), EAA (n=4) and TB (n=2) was examined for glycosaminoglycans and collagen using histochemistry, and for versican, decorin, biglycan, hyaluronan, type I procollagen and alpha-smooth muscle actin using immunohistochemistry. The results showed that in sarcoidosis, EAA and TB, the rim of connective tissue surrounding granulomas contained glycosaminoglycans and collagen, and that glycosaminoglycan staining corresponded to localization of versican. Versican-rich zones contained myofibroblasts that stained intracellularly for type I procollagen. Hyaluronan was found diffusely throughout the matrix. Decorin was localized intracellularly in the epithelioid cells of granulomas and some myofibroblasts. We conclude that the deposition of versican is specific to the early remodelling process in both granulomatous and nongranulomatous lung diseases. In both forms of lung fibrosis, regardless of the nature of the driving inflammatory process, collagen synthesis takes place in a versican-rich provisional matrix. These results suggest that versican may influence the progression of the repair process following many different types of lung injury.

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Section: Discussionsupporting

confidence: 89%

Proteoglycans in granulomatous lung diseases

Bensadoun¹,

Burke²,

Hogg³

et al. 1997

Eur Respir J

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“…Recently, there have been many reports concerning the role of hyaluronic acid during lung development and after injury in pulmonary fibrosis (Bray et al 1991;Juul et al 1994;reviewed in Underhill et al 1993). In summary, increased deposition of hyaluronan is a part of the early changes in the lung during fibrogenesis.…”

Section: Discussionmentioning

confidence: 99%

Expression of CD44 isoforms during bleomycin-or radiation-induced pulmonary fibrosis in rats and mini-pigs

Kasper

Bierhaus

Whyte

et al. 1996

Histochem Cell Biol

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The distribution of CD44s and CD44v molecules in normal and injured lung tissue of rats and mini-pigs was studied by examining the immunohistochemical binding of monoclonal antibodies against CD44 isoforms. We showed that the expression of CD44v and CD44s varies greatly among different pulmonary fibrosis samples and that some tissues express either enhanced expression of CD44s, particularly in the interstitium and on alveolar macrophages, or very low levels of CD44v in the alveolar epithelium. Normal type II pneumocytes expressed the CD44s and CD44v molecules at the basolateral aspect of the cell. Such localisation favours a role for CD44 in epithelial cell-fibroblast interaction during lung development and repair.

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“…Increased HA deposition has been associated ( r = 0.68, = 0.0004). We conclude that 1 ) HA concen-with early injury in bleomycin-injured rat lungs (10). adult RDS tration in RDS lungs of prematurely delivered infant man-( I 1 ).…”

mentioning

confidence: 82%

Changes in Hyaluronan Deposition during Early Respiratory Distress Syndrome in Premature Monkeys

et al. 1994

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ABSTRACT. Increased deposition of hyaluronan (HA) is part of the early response to fibrogenic stimulus in the lung exposed to bleomycin injury and has been associated with increased lung water in adult animals. Early respiratory distress syndrome (RDS) in premature infants is characterized by increased lung water, and late sequelae include fibrosis or bronchopulmonary dysplasia. We hypothesized that increased HA in the alveolar interstitium would be associated with increasingly severe RDS in prematurely delivered monkeys and that modes of therapy that affect severity of disease such a s treatment with high-frequency oscillatory ventilation or exogenous surfactant would decrease this response. Thirty-four Macaca nemestrina mon- processed for microscopy' The presence and severity of array. Extensive intrachain hydrogen bonding, mutual repulsion RDS were evaluated and mOr~hologic criteria. between the negatively charged carboxylate groups, and the HA concentrations in lung extracts increased with progres-linkages between the sugar residues lend HA an inherent stiffness sively severe RDS ( p = 0.qoo3). Treatment with .hfgh-( 1. 2). The large number of hydrophilic residues on the HA chain frequency oscillatory ventilation decreased the lung Injury bind water. resulting in a viscous hydrated gel. Given no conscore (1.69 f 0.7 compared with 2.5 * p = 0.05)3 but straints. a hydrated HA molecule will occupy a volume 1 000 to changes in lung H A concentration did not reach signifi-10 000 times the space of HA alone, The COO-groups along came (37.9 f 22.7 compared with 44.8 f 22-6). Surfactant the molecule also bind cations. increasing the osmotic pressure treatment decreased lung HA concentration (29.6 * 19.0 in the gel. Thus. large amounts of water are taken up in the a t w e t lung) compared with non-surfactant-treated animals presence of HA, increasing the turgor pressure within the matrix.(54.7 * 20.2 ccdg wet lung, P = 0.0009). Two fetal animals HA also interacts or aggregates with other large proteoglycans (144 and 163 d gestation) and seven additional premature forming even larger complexes. In addition to its space-filling animals ventilated for up to 96 h were compared with the role. HA can act as a specific ligand for HA receptors. Two such animals killed a t 6 h. H A concentrations increased with receptors have been identified: CD44 (3) and RHAMM (4). The length of mechanical ventilation and severity of illness in interaction of HA with these receptors mediates several functions these animals. HA was localized in freeze-dried lung sec-during normal cellular metabolism (5). as well as during develtions using a biotinylated probe. Lung sections were blindly opmental (6, 7) and repair processes (8). scored for the distribution of H A staining, and these scoresIn normal mature lung, HA is present at the basal surfaces of were positively correlated with HA concentration measure-bronchiolar epithelium and in the adventitia of muscularized merits ( r = 0.75, p < 0.0001). The quantity of H A in blood vessels. whereas l...

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Early Changes in Lung Tissue Hyaluronan (Hyaluronic Acid) and Hyaluronidase in Bleomycin-induced Alveolitis in Hamsters

Cited by 47 publications

References 13 publications

Proteoglycans in granulomatous lung diseases

Proteoglycans in granulomatous lung diseases

Expression of CD44 isoforms during bleomycin-or radiation-induced pulmonary fibrosis in rats and mini-pigs

Changes in Hyaluronan Deposition during Early Respiratory Distress Syndrome in Premature Monkeys

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