Early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbits

Camejo, Germán; Bosch, Virgilio; C, Arreaza; Mendez, Halina C.

doi:10.1016/s0022-2275(20)39330-5

Cited by 89 publications

(5 citation statements)

References 17 publications

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“…Animal models have been used extensively to study the genesis and progression of atherosclerosis (Clarkson, 1963). The rabbit has been particularly useful, since marked hypercholesterolemia can be induced within a few days by supplementing rabbit chow diets with cholesterol (Ross & Zilversmit, 1977;Shore et al, 1974;Camejo et al, 1973). The increase in serum cholesterol (mainly cholesteryl esters) occurs primarily in particles that float in the VLDL density range (d < 1.006 g/mL) and in the LDL density range (1.006 < d < 1.063 g/mL).…”

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confidence: 99%

A carbon-13 NMR study of aortic lesions and cholesteryl ester rich lipoproteins from atherosclerotic rabbits

Kroon¹,

Quinn²,

Cordes³

1982

Biochemistry

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When rabbits are fed a diet supplemented with cholesterol, their plasma cholesterol levels increased markedly and they developed atherosclerosis. Most of the plasma cholesterol exists as cholesteryl esters in very low density and low-density lipoproteins. Carbon-13 nuclear magnetic resonance (13C NMR) spectra (at 48 "C) of lipoproteins, and of arterial lesions from cholesterol-fed rabbits, are dominated by well-resolved cholesteryl ester resonances. An analysis of their line widths shows that the cholesteryl esters are in a liquid state at this temperature. Calculations based on line widths and spin-lattice relaxation times show that the motion of the cholesteryl ester molecules is highly anisotropic; motion about the long axis of the cholesteryl moiety is 38-75 times faster than motion about the short axis. Spectra for the lipoproteins and arterial lesions show temperature-dependent line-width

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confidence: 99%

A carbon-13 NMR study of aortic lesions and cholesteryl ester rich lipoproteins from atherosclerotic rabbits

Kroon¹,

Quinn²,

Cordes³

1982

Biochemistry

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“…The decrease in spectral amplitude for VLDL, IDL, and LDL from cholesterol-fed rabbits between 55 and 15 °C shows that the mobility of the core cholesteryl esters is tem-( holesterol feeding in rabbits produces hypercholesterolemia and atherosclerosis (Anitschkow, 1933;Shumaker, 1956). Hypercholesterolemia is accompanied by a large increase in the VLDL1 and IDL cholesterol concentrations and a moderate increase in the LDL cholesterol concentration (Shumaker, 1956;Camejo et al, 1973;Shore et al, 1974). HDL cholesterol levels have been reported to decrease (Shore et al, 1974) or to remain constant (Pinon & Bridonx, 1977).…”

mentioning

confidence: 99%

“…HDL cholesterol levels have been reported to decrease (Shore et al, 1974) or to remain constant (Pinon & Bridonx, 1977). Cholesterol feeding also has a major effect on the composition of the lipoprotein particles (Camejo et al, 1973;Shore et al, 1974; Pinon & Bridonx, 1977;Rodriguez et al, 1976). Camejo et al (1973) have shown that the VLDL cholesteryl ester content increased from 4.3% to 40.3% while the triglyceride content decreased from 54.1% to 15.1% when rabbits were fed a diet supplemented with 1% cholesterol.…”

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confidence: 99%

“…Cholesterol feeding also has a major effect on the composition of the lipoprotein particles (Camejo et al, 1973;Shore et al, 1974; Pinon & Bridonx, 1977;Rodriguez et al, 1976). Camejo et al (1973) have shown that the VLDL cholesteryl ester content increased from 4.3% to 40.3% while the triglyceride content decreased from 54.1% to 15.1% when rabbits were fed a diet supplemented with 1% cholesterol. Such compositional changes can have a profound effect on the structural characteristics of the lipoprotein core structure (Deckelbaum et al, 1977a).Studies using techniques such as low angle X-ray scattering, differential scanning calorimetry (DSC), and nuclear magnetic resonance (NMR) spectroscopy (Deckelbaum et al, 1977a;…”

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confidence: 99%

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Organization of the core lipids of lipoproteins from normal and cholesterol-fed rabbits. A proton nuclear magnetic resonance study

Kroon¹,

Seidenberg²

1982

Biochemistry

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When rabbits are fed a diet supplemented with cholesterol, their plasma cholesterol levels increase markedly, and they develop atherosclerosis. Most of the plasma cholesterol exists as cholesteryl esters in very low density lipoproteins (VLDL) and intermediate-density lipoproteins (IDL). The triglyceride content of the lipoprotein cores decreases sharply during cholesterol feeding. This change is most marked for VLDL in which it decreases from 74% to 5%, while the cholesteryl ester content increases from 26% to 95%. The IDL and low-density lipoprotein (LDL) fractions from cholesterol-fed rabbits have a triglyceride content of 2% or less in their cores. The mobility of the core cholesteryl esters has been studied by proton nuclear magnetic resonance spectroscopy. Changes in the mobility were assessed by measuring the temperature dependence of the amplitude of the methylene resonances. The decrease in spectral amplitude for VLDL, IDL, and LDL from cholesterol-fed rabbits between 55 and 15 degrees C shows that the mobility of the core cholesteryl esters is temperature dependent and that the cholesteryl esters display thermal order--disorder transitions with midpoints of 42, 40, and 38 degrees C, respectively. At physiological temperatures, the core cholesteryl esters of lipoproteins from cholesterol-fed rabbits therefore exist in a partially ordered state. In contrast, the core cholesteryl esters of VLDL, IDL, and LDL from normal rabbits show no evidence for an order--disorder transition. This is consistent with their high core triglyceride content which precludes the existence of an ordered cholesteryl ester phase within the core. The core cholesteryl esters of normal rabbit lipoproteins therefore exist in a liquid state at physiological temperatures. High-density lipoproteins (HDL) from normal and cholesterol-fed rabbits fail to display an order--disorder transition. This is attributed to the constraints imposed by the small HDL core diameter, which prevents the existence of an ordered arrangement of cholesteryl esters, irrespective of the core triglyceride content.

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“…Studies of apoE turnover in vivo (Wong & Rubinstein, 1979) suggest that there is increased production of this apolipoprotein. Experiments on isolated perfused livers as well as on liver slices in vitro (Roheim et al, 1963;Camejo et al, 1973) suggest that probably the synthesis of various apolipoproteins may be increased by cholesterol feeding of the animals. However, these studies have generally been indirect and do not address the question of the biosynthetic rate of specific individual apolipoproteins.…”

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Effects of an atherogenic diet on apolipoprotein E biosynthesis in the rat

Lin-Lee¹,

Tanaka²,

Lin³

et al. 1981

Biochemistry

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Early changes in plasma lipoprotein structure and biosynthesis in cholesterol-fed rabbits

Cited by 89 publications

References 17 publications

A carbon-13 NMR study of aortic lesions and cholesteryl ester rich lipoproteins from atherosclerotic rabbits

A carbon-13 NMR study of aortic lesions and cholesteryl ester rich lipoproteins from atherosclerotic rabbits

Organization of the core lipids of lipoproteins from normal and cholesterol-fed rabbits. A proton nuclear magnetic resonance study

Effects of an atherogenic diet on apolipoprotein E biosynthesis in the rat

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