Early compaction at day 3 may be a useful additional criterion for embryo transfer

Cruguel, Sébastien Le; Ferré-L’Hôtellier, Véronique; Morinière, Catherine; Lemerle, S.; Reynier, Pascal; Descamps, Philippe; May‐Panloup, Pascale

doi:10.1007/s10815-013-9983-3

Cited by 17 publications

(11 citation statements)

References 39 publications

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“…The reduced proportion of high-quality embryos on day 3 could negatively influence the likelihood of progression to the blastocyst stage and, in turn, the likelihood of pregnancy and implantation (23). The decreased rate of compaction on day 3 in the dry culture could have resulted in a lower implantation rate and poorer overall clinical outcomes in this group because of the known correlation between compaction and implantation rates (24).…”

Section: Discussionmentioning

confidence: 99%

Humid versus dry incubator: a prospective, randomized, controlled trial

Fawzy¹,

Abdel-Rahman

Zidan

et al. 2017

Fertility and Sterility

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Section: Discussionmentioning

confidence: 99%

Humid versus dry incubator: a prospective, randomized, controlled trial

Fawzy¹,

Abdel-Rahman

Zidan

et al. 2017

Fertility and Sterility

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“…Among the embryos that developed to more than 16 cell stages, morula is characterized by its appearance with unclear outlines of blastomeres following the process of compaction, and observed from day 3 or 4 after fertilization [42]. In fact, embryonic genome activation is known to generally occur in a part of morula stage, which causes embryos that undergo this phase to possibly proceed to compaction and cavitation [43]. Transcribed genes in this stage, engaged in metabolism of amino acids, carbohydrates, lipids, and proteins, regulate the system of energy metabolism in embryos, which is required for consequent development into BL [44].…”

Section: Discussionmentioning

confidence: 99%

Anti-Oxidative Effects of Human Adipose Stem Cell Conditioned Medium with Different Basal Medium during Mouse Embryo In Vitro Culture

Kim

et al. 2020

Animals

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The quality of embryos produced by assisted reproductive techniques should be advanced by the improvement of in vitro culture conditions for successful implantation and pregnancy maintenance. We investigated the anti-oxidative effect of human adipose stem cell (ASC) conditioned medium with its optimal basal medium, Dulbecco′s modified Eagle′s medium (DMEM-CM), or keratinocyte serum-free medium (KSFM-CM) as supplements during in vitro culture (IVC) of in vitro fertilized mouse embryo. At first, preimplantation embryo development was evaluated in KSFM-CM and DMEM-CM supplemented cultures at various concentrations. The blastocyst (BL) and hatched BL formation rates were significantly increased in 5% DMEM-CM, while no difference was observed from KSFM-CM. Next, comparing the efficacy of KSFM-CM and DMEM-CM at the same concentration, DMEM-CM enhanced the developmental rate of 16 cells, morula, BL, and hatched BL. The expression level of reactive oxygen species decreased and that of glutathione increased in BL cultured with DMEM-CM, which confirms its anti-oxidative effect. Furthermore, apoptosis in BL cultured with DMEM-CM was reduced compared with that in KSFM-CM. This study demonstrated that the comparative effect of human ASC-CM made of two different basal media during mouse embryo IVC and anti-oxidative effect of 5% DMEM-CM was optimal to improve preimplantation embryo development.

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“…Embryos that undergo compaction and cavitation become distinguishable and reflect embryo commitment to the next stage of development [ 56 ]. It has been reported that embryo quality on day 2 may be linked to the ability to undergo compaction on day 3 [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Prediction of blastocyst development and implantation potential <i>in utero</i> based on the third cleavage and compaction times in mouse pre-implantation embryos

Kim

Jun

2017

Journal of Reproduction and Development

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Cytokinesis and cell division during pre-implantation embryonic development occur as an orchestrated spatiotemporal program. Cleavage, compaction, and blastulation in pre-implantation embryos are essential for successful implantation and pregnancy. Their alteration is associated with chromosomal imbalance and loss of developmental competence. In this study, we evaluated the time of cleavage and compaction as predictors for in vitro pre- and peri-implantation development and in utero implantation potential by time-lapse monitoring. Mouse 2-cell embryos were collected on 1.5 days post coitum (dpc) and were individually cultured to the outgrowth (OG) stage (7.5 dpc). Developmental stages were classified as 3-cell, 4-cell, 8-cell, morula, blastocyst, and OG. Cut-off times for successful blastocyst development were determined by receiver operating characteristic curve analysis. When cut-off times were set as 9 h for the third cleavage from the 2- to 4-cell stage, and 40 h for compaction from the 2-cell to morula stage, blastocyst and OG development rates, respectively, were significantly higher (P < 0.0001). Embryos were grouped according to the above cut-off time and transferred to the contralateral uterine horn on 3.5 dpc. Implantation rates in utero on 5.5 dpc were significantly higher in early third cleaved (≤ 9 h from 2- to 4-cell) and early compacted embryos (≤ 40 h from 2-cell to morula) than those in delayed embryos (P < 0.05). Therefore, the time of the third cleavage from 2- to the 4-cell stage and compaction from 2-cell to morula stage may be a useful morphokinetic parameter for predicting developmental potential, including successful implantation and pregnancy in human in vitro fertilization-embryo transfer programs.

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Early compaction at day 3 may be a useful additional criterion for embryo transfer

Abstract: Thus, early compaction, sometimes observed at day 3, may serve as a useful additional criterion for selecting the embryos transferred.

Cited by 17 publications

References 39 publications

Humid versus dry incubator: a prospective, randomized, controlled trial

Humid versus dry incubator: a prospective, randomized, controlled trial

Anti-Oxidative Effects of Human Adipose Stem Cell Conditioned Medium with Different Basal Medium during Mouse Embryo In Vitro Culture

Prediction of blastocyst development and implantation potential <i>in utero</i> based on the third cleavage and compaction times in mouse pre-implantation embryos

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