Early dengue diagnosis by nonstructural protein 1 antigen detection: Rapid immunochromotography versus two the enzyme-linked immunosorbent assay kits

Spellman, Stephen R.; Charles, Marie Victor Pravin; Anitharaj, Velmurugan; Deepa, C; Umadevi, S.

doi:10.4103/0377-4929.130905

Cited by 17 publications

(13 citation statements)

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“…Maximum seropositive patients 50 (55.56%) belonged to the age group of 15-45 years composed of young-working male population. Similar data was obtained by a study conducted by Stephen et al, 2014. The reactivity gradually decreased towards extremes of age group [ Table 1].…”

Section: Resultssupporting

confidence: 88%

Dengue NS1 Antigen – An Important Early Diagnostic Tool for Diagnosis of Dengue Infection

Das¹,

Turbadkar²,

Baveja³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 88%

Dengue NS1 Antigen – An Important Early Diagnostic Tool for Diagnosis of Dengue Infection

Das¹,

Turbadkar²,

Baveja³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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“…Among these tests, the Panbio early dengue NS1 capture ELISA has shown to have an overall sensitivity of 60.4 to 66% and a specificity of 97.9 to 99% [10]-[12]. It has also been shown that NS1 rapid antigen detection tests (SD Diagnostics, Bioline, South Korea) has a [13] sensitivity of 81.6% and a specificity of 92% [14]. …”

Section: Introductionmentioning

confidence: 99%

Dengue NS1 antigen as a marker of severe clinical disease

et al. 2014

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BackgroundEarly detection of complications significantly reduces dengue associated mortality and morbidity. We set out to determine if the NS1 rapid antigen detection test could be used as a point of care test to predict severe disease.Methods186 adult patients with confirmed dengue were enrolled during day 3-8 of illness. Clinical and laboratory parameters were recorded during the course of the illness and NS1 antigen levels were determined using both the Panbio dengue early ELISA (Panbio, Australia) and a NS1 rapid antigen detection kit (SD Bioline, South Korea).Results59.1% of patients presented to hospital on day 5-6 of illness when NS1 antigen positivity was significantly (p = 0.008) associated with severe dengue (odds ratio 3.0, 95% CI 1.39 to 6.47) and the NS1 antigen levels were significantly higher (p = 0.03) in those who went on to develop shock. Serum NS1 antigen levels significantly (p < 0.0001) and inversely correlated with the total white cell counts and lymphocyte counts. The bedside NS1 test showed comparable sensitivity (97.4%) and specificity (93.7%) to the laboratory NS1 test in our setting and cohort.ConclusionNS1 antigen positivity is associated with a higher risk of developing severe dengue especially when positive beyond day 5 of illness in our cohort, and while further validation studies are required, the test can therefore potentially be used as a bedside point of care test as a warning sign of severe dengue.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-014-0570-8) contains supplementary material, which is available to authorized users.

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“…In fact, the problem of false-negative in dengue diagnosis is an important issue for discussion. First, the problem of the NS1 assay in dengue diagnosis is noted worldwide 2 . Combining with other immunological test is suggested for improvement of diagnostic accuracy 2 .…”

mentioning

confidence: 99%

“…First, the problem of the NS1 assay in dengue diagnosis is noted worldwide 2 . Combining with other immunological test is suggested for improvement of diagnostic accuracy 2 . Also, it should be noted that the diagnosis of dengue in many tropical countries is usually a presumptive diagnosis without the laboratory confirmation 3 .…”

mentioning

confidence: 99%