Early detection of pancreatic cancers in liquid biopsies by ultrasensitive fluorescence nanobiosensors

Early detection of non-small cell lung cancer in liquid biopsies by ultrasensitive protease activity analysis

“…It is established that virtually all solid tumors are characterized by dysfunctional protease expression patterns [20][21][22] . These deviations from the proteasome of healthy cells have been successfully used by the authors for the early detection of breast [5] and pancreatic [7] cancer. In cancer, numerous proteases, such as matrix metalloproteinases (MMPs) [23] , cathepsins [24][25][26] , and urokinase plasminogen activator (uPA) [20,22,25] are either over-or underexpressed, when compared to healthy cells.…”

“…In cancer, numerous proteases, such as matrix metalloproteinases (MMPs) [23] , cathepsins [24][25][26] , and urokinase plasminogen activator (uPA) [20,22,25] are either over-or underexpressed, when compared to healthy cells. This enables the detection of solid tumors in liquid biopsies through the simultaneous detection of several proteases in serum [5,7] . The Bossmann group has continuously developed their patented technology for ultra-sensitive protease detection since 2007 [27][28][29] .…”

“…On the basis of the 5-year survival statistics of NSCLC, we conservatively estimate that about 30 percent of mortalities could be prevented if NSCLC were routinely detected at stage 1 (combined T1a, T1b, and T1c) instead at stages 2 or 3, when it is currently diagnosed [3] . The optical nanobiosensors developed in the Bossmann group feature sub-femtomolar limits of detection, thereby permitting the diagnosis of NSCLC by means of a liquid biopsy utilizing the serum of cancer patients [4][5][6][7] .…”

Magnetic Nanomaterials for Magnetically-Aided Drug Delivery and Hyperthermia

“…Bossmann and co-workers have developed fluorescence-based nanobiosensors for the detection of solid tumors in liquid biopsies and protease detection in blood [18,22,[76][77][78]. These nanobiosensors feature Fe/Fe 3 O 4 core/shell nanoparticles, which were synthesized by using thermal decomposition of Fe(CO) 5 in the presence of oleylamine and hexadecylammonium chloride (HADxHCl) using 1-octadecene (ODE) as solvent [79].…”

“…Magnetic nanoparticles play a big role in cancer detection [7][8][9], drug delivery [2,3], as well as hyperthermia treatment [10][11][12]. During the last decade, bi-magnetic core/shell nanoparticles featuring an iron core and an iron oxide (Fe 3 O 4 ) shell have become widely used [13][14][15][16][17][18][19], due to their superior magnetic properties [20,21].…”