Early development of leg and wing primordia in the Drosophila embryo

Cohen, B. A.; Wimmer, Ernst A.; Cohen, Stephen M.

doi:10.1016/0925-4773(91)90030-a

Cited by 160 publications

(220 citation statements)

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“…One proposed function for the later expression of dpp in the lateral ectoderm is the specification of the imaginal disk primordia (Cohen et al, 1993). It was proposed that the intersection of dpp and wingless expression patterns in the lateral ectoderm causes localized expression of distalless and perhaps other genes required for imaginal disk development.…”

Section: Discussionmentioning

confidence: 99%

Embryonic expression patterns of the drosophila decapentaplegic gene: Separate regulatory elements control blastoderm expression and lateral ectodermal expression

Jackson

Hoffmann

1994

Developmental Dynamics

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Patterns of decapentaplegic (dpp) transcripts derived from the intact gene were compared to the patterns of transcripts generated by partial dpp transgenes in Drosophila embryos. Sequences closest to the dpp coding regions, the dpp hin region, were sufficient to express ZacZ-tagged mRNA in patterns indistinguishable from the patterns of endogenous dpp expression in the dorsal and terminal cells at the blastoderm stage, in the dorsal ectoderm during germ band elongation, and in narrow stripes of ectodermal cells along the dorsal edge of the ectoderm and at the boundary between the lateral and ventral neurogenic regions during germ band shortening. The latter pattern of expression responded to the segment polarity genes naked and wingless. However, these dpp sequences were not sufficient to drive ZucZ-tagged mRNA expression in other cells normally expressing dpp, including cells in the gnathal segments, the clypeolabrum, the foregut, the midgut visceral mesoderm, and the hindgut. Two separate regulatory regions were found in the dpp hin region. A 479 bp region upstream of the promoter was necessary for the segmented pattern of expression in the lateral ectoderm and for expression in the midgut endoderm. Cis-acting elements in the 2 kbp second intron directed expression in the dorsal and terminal regions of the blastoderm, acted on a heterologous promoter, the P-element promoter, and responded to pattern information derived from the maternal effect dorsal/ventral patterning genes. 0 1994 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Embryonic expression patterns of the drosophila decapentaplegic gene: Separate regulatory elements control blastoderm expression and lateral ectodermal expression

Jackson

Hoffmann

1994

Developmental Dynamics

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“…In the larval leg disc, wg is expressed along the ventral portion of the anterior-posterior compartment boundary (Cohen et al 1993), where it determines ventral cell fates and distal outgrowth of the leg (Campbell et al 1993;Struhl and Basler 1993;Wilder and Perrimon 1995). During wing development, wg functions at several stages, reflecting the sequential compartmentalization of the wing disc.…”

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confidence: 99%

eyelidantagonizes wingless signaling during Drosophiladevelopment and has homology to the Bright family of DNA-binding proteins

Treisman

Luk²,

Rubin³

et al. 1997

Genes Dev.

115

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In Drosophila, pattern formation at multiple stages of embryonic and imaginal development depends on the same intercellular signaling pathways. We have identified a novel gene, eyelid (eld), which is required for embryonic segmentation, development of the notum and wing margin, and photoreceptor differentiation. In these tissues, eld mutations have effects opposite to those caused by wingless (wg) mutations. eld encodes a widely expressed nuclear protein with a region homologous to a novel family of DNA-binding domains. Based on this homology and on the phenotypic analysis, we suggest that Eld could act as a transcription factor antagonistic to the Wg pathway.

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“…Shortly after induction, the thoracic disc primordia separate into the dorsal (wings and halteres) and ventral (legs) imaginal precursors (Cohen et al, 1993). As the dorsal and ventral disc primordia separate, changes in gene expression occur that begin the process of patterning the adult legs; the current model is summarized in Fig.…”

Section: Introductionmentioning

confidence: 99%

“…Other genes are expressed in the imaginal discs primordia during early embryogenesis, such as the paralogous pair disconnected (disco) and disco-related (disco-r) (Cohen et al, 1991;Lee et al, 1991;Mahaffey et al, 2001), together referred to as "the disco genes" hereafter. The disco genes encode redundant C2H2 zinc finger transcription factors (Mahaffey et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…) and body wall of the adult insect during the pupal stage. The thoracic imaginal discs form during embryogenesis when segmental expression of the morphogen Wingless (Wg) induces a small group of cells to activate appendage-specific gene networks (Cohen et al, 1993;Cohen, 1990;Estella and Mann, 2010;Estella et al, 2003). The dorsal limit of the disc primordia is set through repression by Decapentaplegic (Dpp) and the ventral limit by repression from the Epidermal Growth Factor Receptor (EGFR) (Cohen et al, 1993;Goto and Hayashi, 1997;Kubota et al, 2000;Raz and Shilo, 1993).…”

Section: Introductionmentioning

confidence: 99%

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The Drosophila genesdisconnectedanddisco-relatedtogether specify development of adult legs

2016

Preprint

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Running Title: Drosophila leg disco/disco-r Keywords: Drosophila, appendage, disco, leg, Distal-less . CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/052811 doi: bioRxiv preprint first posted online May. 11, 2016; 2 ABSTRACTIn the fruit fly, Drosophila melanogaster, specification of the legs begins during embryogenesis when Wingless signaling induces small groups of cells to form the imaginal disc primordia in the thoracic segments. This signal initiates expression of transcription factors that will later be used to pattern the legs. The paralogous genes disconnected and disco-related encode transcription factors that are expressed in the disc primordia during early embryogenesis, and their expression continues in the leg discs during larval and pupal stages. The importance of these two genes in establishing the leg development trajectory was indicated by our previous observation that ectopic expression of either gene in the wing discs cells caused legs to develop in place of wings. However, because of their redundancy and requirement for survival during embryogenesis, we were unable to define their role in development of the adult legs. Here, we report loss-offunction analyses of the disco genes during development of the legs. We discovered that loss of both genes' functions causes both truncation of the distal leg with apparent overgrowth of proximal regions and complete loss of legs and ventral thoracic body patterning. At the molecular level we noted reduction or loss of signaling and transcription factors that pattern the proximal-distal axis of the legs. We conclude from these studies that the disco genes promote leg development through regulation of signaling processes, but also by stabilizing expression of the leg determination gene network.

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Early development of leg and wing primordia in the Drosophila embryo

Cited by 160 publications

References 49 publications

Embryonic expression patterns of the drosophila decapentaplegic gene: Separate regulatory elements control blastoderm expression and lateral ectodermal expression

Embryonic expression patterns of the drosophila decapentaplegic gene: Separate regulatory elements control blastoderm expression and lateral ectodermal expression

eyelidantagonizes wingless signaling during Drosophiladevelopment and has homology to the Bright family of DNA-binding proteins

The Drosophila genesdisconnectedanddisco-relatedtogether specify development of adult legs

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