Early developmental expression and experimental axis determination by the chicken Vg1 gene

Seleiro, E. A. P.; Connolly, David; Cooke, Jonathan

doi:10.1016/s0960-9822(96)00752-x

Cited by 97 publications

(53 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As depicted in Fig. 9A, during midgastrulation (stage 4), Vg1 and Nodal are expressed throughout the primitive streak with the highest concentration in the midstreak, the co-receptor Cripto is expressed in the prospective PM and IM regions of the streak, and the Nodal-like signaling inhibitor Lefty is expressed in a gradient pattern with the highest levels in the most anterior portion of the streak (Ishimaru et al, 2000;Joubin and Stern, 1999;Lawson et al, 2001;Levin et al, 1995;Seleiro et al, 1996). If these patterns are considered together, a picture emerges in which Nodal-like signaling activity during midgastrulation is predicted to be highest in the mid-primitive streak (the source of the IM), consistent with the current observation that Nodal-like signaling promotes IM formation during this stage of development.…”

Section: Research Articlementioning

confidence: 99%

A role for Vg1/Nodal signaling in specification of the intermediate mesoderm

et al. 2013

View full text Add to dashboard Cite

SUMMARYThe intermediate mesoderm (IM) is the embryonic source of all kidney tissue in vertebrates. The factors that regulate the formation of the IM are not yet well understood. Through investigations in the chick embryo, the current study identifies and characterizes Vg1/Nodal signaling (henceforth referred to as 'Nodal-like signaling') as a novel regulator of IM formation. Excess Nodal-like signaling at gastrulation stages resulted in expansion of the IM at the expense of the adjacent paraxial mesoderm, whereas inhibition of Nodallike signaling caused repression of IM gene expression. IM formation was sensitive to levels of the Nodal-like pathway co-receptor Cripto and was inhibited by a truncated form of the secreted molecule cerberus, which specifically blocks Nodal, indicating that the observed effects are specific to the Nodal-like branch of the TGFβ signaling pathway. The IM-promoting effects of Nodal-like signaling were distinct from the known effects of this pathway on mesoderm formation and left-right patterning, a finding that can be attributed to specific time windows for the activities of these Nodal-like functions. Finally, a link was observed between Nodal-like and BMP signaling in the induction of IM. Activation of IM genes by Nodal-like signaling required an active BMP signaling pathway, and Nodal-like signals induced phosphorylation of Smad1/5/8, which is normally associated with activation of BMP signaling pathways. We postulate that Nodal-like signaling regulates IM formation by modulating the IM-inducing effects of BMP signaling.

show abstract

Section: Research Articlementioning

confidence: 99%

A role for Vg1/Nodal signaling in specification of the intermediate mesoderm

et al. 2013

View full text Add to dashboard Cite

show abstract

“…These include Activin (Mitrani et al, 1990), Vg1 (Seleiro et al, 1996;Shah et al, 1997), Wnt8c (Hume and Dodd, 1993;Seleiro et al, 1996;Shah et al, 1997), and Chordin (Streit et al, 1998). All these factors are expressed in the marginal zone of the blastodisc, including the posterior region that exhibits the streak-inducing activity.…”

Section: Induction Of the Initial Primitive Streakmentioning

confidence: 99%

Induction and patterning of the primitive streak, an organizing center of gastrulation in the amniote

Matsukawa

Poh

Kelly

et al. 2004

Developmental Dynamics

View full text Add to dashboard Cite

The primitive streak is the organizing center for amniote gastrulation. It defines the future embryonic midline and serves as a conduit of cell migration for germ layer formation. The migration patterns of endodermal and mesodermal precursors through the streak have been studied in great detail. Additional new breakthroughs recently have revealed the cell biological and molecular mechanisms that govern streak induction and patterning. These findings include (1) identification of the ontogeny and inductive signals of streak precursors, (2) the potential cellular mechanism of streak extension, and (3) the molecular and functional diversification along the anterior-posterior and mediolateral axes within the primitive streak. These findings indicate that amniote embryos initiate gastrulation by using both evolutionarily conserved and divergent mechanisms. The data also provide a foundation for understanding how the midline axis is defined and maintained during gastrulation of the amniotes. Developmental Dynamics 229:422-432, 2004.

show abstract

“…A messenger RNA encoding Vg1, a member of the TGF-␤ superfamily, was the first example of an asymmetrically localized embryonic RNA (Weeks and Melton, 1987), adding momentum to the idea that growth factors might be morphogens. (Ironically, despite many efforts, no morphogen function for Vg1 has yet been found in the frog, although it may have a role in leftright patterning [Hyatt et al, 1996] and an apparent orthologue may play a role in axial patterning in the chick [Seleiro et al, 1996;Shah et al, 1997]). The TGF-␤ superfamily member that emerged first as a mesoderm-inducing factor par excellence was activin ; although this is probably not the endogenous inducer-morphogen; see below).…”

Section: Xenopus and Growth Factor Morphogensmentioning

confidence: 99%

Morphogen gradients, positional information, and Xenopus: Interplay of theory and experiment

Green

2002

Developmental Dynamics

View full text Add to dashboard Cite

The idea of morphogen gradients has long been an important one in developmental biology. Studies with amphibians and with Xenopus in particular have made significant contributions to demonstrating the existence, identity, and mechanisms of action of morphogens. Mesoderm induction and patterning by activin, nodals, bone morphogenetic proteins, and fibroblast growth factors have been analyzed thoroughly and reveal recurrent and combinatorial roles for these protein growth factor morphogens and their antagonists. The dynamics of nodal-type signaling and the intersection of VegT and ␤-catenin intracellular gradients reveal detailed steps in early long-range patterning. Interpretation of gradients requires sophisticated mechanisms for sharpening thresholds, and the activin-Xbra-Gsc system provides an example of this. The understanding of growth factor signal transduction has elucidated growth factor morphogen action and provided tools for dissecting their direct longrange action and distribution. The physical mechanisms of morphogen gradient establishment are the focus of new interest at both the experimental and theoretical level. General themes and emerging trends in morphogen gradient studies are discussed.

show abstract

Early developmental expression and experimental axis determination by the chicken Vg1 gene

Abstract: In vertebrate development, Vg genes may be required for an evolutionarily conserved early step in positioning or induction of the axis.

Cited by 97 publications

References 44 publications

A role for Vg1/Nodal signaling in specification of the intermediate mesoderm

A role for Vg1/Nodal signaling in specification of the intermediate mesoderm

Induction and patterning of the primitive streak, an organizing center of gastrulation in the amniote

Morphogen gradients, positional information, and Xenopus: Interplay of theory and experiment

Contact Info

Product

Resources

About