Early diagnosis of a Mexican variant of Papaya meleira virus (PMeV-Mx) by RT-PCR

Zamudio-Moreno, Emily; Ramírez-Prado, Jorge Humberto; Moreno‐Valenzuela, Oscar A.; López-Ochoa, Luisa

doi:10.4238/2015.february.6.18

Cited by 17 publications

(7 citation statements)

References 11 publications

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“…A disease reported since the 1980s in Brazil, named meleira disease, or "sticky disease," of papaya, caused by the papaya meleira virus (PMeV) (16), was reported in Yucatan, Mexico (17,18). Recently, papaya meleira virus 2 (PMeV-2), with etiology similar to that of meleira disease (19), was discovered but is not yet known to cause problems for papaya production in Mexico. Plant-virus interactions can have devastating outcomes; however, interactions between viruses can also be antagonistic, as we recently reported in a time series study of coinfection of PapMV and PRSV (20).…”

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confidence: 99%

Network Analysis of the Papaya Orchard Virome from Two Agroecological Regions of Chiapas, Mexico

Alcalá-Briseño

Casarrubias-Castillo²,

López-Ley³

et al. 2020

mSystems

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The study of complex ecological interactions, such as those among host, pathogen, and vector communities, can help to explain host ranges and the emergence of novel pathogens. We evaluated the viromes of papaya orchards, including weed and insect viromes, to identify common viruses in intensive production of papaya in the Pacific Coastal Plain and the Central Depression of Chiapas, Mexico. Samples of papaya cultivar Maradol, susceptible to papaya ringspot virus (PRSV), were categorized by symptoms by local farmers (papaya ringspot symptoms, non-PRSV symptoms, or asymptomatic). These analyses revealed the presence of 61 viruses, where only 4 species were shared among both regions, 16 showed homology to known viruses, and 36 were homologous with genera including Potyvirus, Comovirus, and Tombusvirus (RNA viruses) and Begomovirus and Mastrevirus (DNA viruses). We analyzed the network of associations between viruses and host-location combinations, revealing ecological properties of the network, such as an asymmetric nested pattern, and compared the observed network to null models of network association. Understanding the network structure informs management strategies, for example, revealing the potential role of PRSV in asymptomatic papaya and that weeds may be an important pathogen reservoir. We identify three key management implications: (i) each region may need a customized management strategy; (ii) visual assessment of papaya may be insufficient for PRSV, requiring diagnostic assays; and (iii) weed control within orchards may reduce the risk of virus spread to papaya. Network analysis advances understanding of host-pathogen interactions in the agroecological landscape. IMPORTANCE Virus-virus interactions in plants can modify host symptoms. As a result, disease management strategies may be unsuccessful if they are based solely on visual assessment and diagnostic assays for known individual viruses. Papaya ringspot virus is an important limiting factor for papaya production and likely has interactions with other viruses that are not yet known. Using high-throughput sequencing, we recovered known and novel RNA and DNA viruses from papaya orchards in Chiapas, Mexico, and categorized them by host and, in the case of papaya, symptom type: asymptomatic papaya, papaya with ringspot virus symptoms, papaya with nonringspot symptoms, weeds, and insects. Using network analysis, we demonstrated virus associations within and among host types and described the ecological community patterns. Recovery of viruses from weeds and asymptomatic papaya suggests the need for additional management attention. These analyses contribute to the understanding of the community structure of viruses in the agroecological landscape.

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mentioning

confidence: 99%

Network Analysis of the Papaya Orchard Virome from Two Agroecological Regions of Chiapas, Mexico

Alcalá-Briseño

Casarrubias-Castillo²,

López-Ley³

et al. 2020

mSystems

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“…PMeV2-ES has high similarity to PMeV-Mx and to PpVQ, a new umbra-like virus recently described in Ecuador [ 34 ]. PMeV-Mx was detected in papaya plants showing typical symptoms of sticky disease in Mexico [ 12 ]. PpVQ was detected in papaya plants also infected by Papaya ringspot virus (PRSV).…”

Section: Discussionmentioning

confidence: 99%

“…However, a cDNA library generated from papaya latex infected by a Mexican isolate (named PMeV-Mx) identified a 1154-bp sequence encoding a putative RdRp showing closer similarity to umbraviruses and no similarity to PMeV-RN. Nevertheless, primers designed based on the PMeV-Mx sequence amplified fragments from both Brazilian and Mexican infected plants, and the amplified sequences had 100% identity at the nucleotide level [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease

et al. 2016

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Papaya sticky disease, or “meleira”, is one of the major diseases of papaya in Brazil and Mexico, capable of causing complete crop loss. The causal agent of sticky disease was identified as an isometric virus with a double stranded RNA (dsRNA) genome, named papaya meleira virus (PMeV). In the present study, PMeV dsRNA and a second RNA band of approximately 4.5 kb, both isolated from latex of papaya plants with severe symptoms of sticky disease, were deep-sequenced. The nearly complete sequence obtained for PMeV dsRNA is 8,814 nucleotides long and contains two putative ORFs; the predicted ORF1 and ORF2 display similarity to capsid proteins and RdRp's, respectively, from mycoviruses tentatively classified in the family Totiviridae. The sequence obtained for the second RNA is 4,515 nucleotides long and contains two putative ORFs. The predicted ORFs 1 and 2 display 48% and 73% sequence identity, respectively, with the corresponding proteins of papaya virus Q, an umbravirus recently described infecting papaya in Ecuador. Viral purification in a sucrose gradient allowed separation of particles containing each RNA. Mass spectrometry analysis indicated that both PMeV and the second RNA virus (named papaya meleira virus 2, PMeV2) were encapsidated in particles formed by the protein encoded by PMeV ORF1. The presence of both PMeV and PMeV2 was confirmed in field plants showing typical symptoms of sticky disease. Interestingly, PMeV was detected alone in asymptomatic plants. Together, our results indicate that sticky disease is associated with double infection by PMeV and PMeV2.

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“…While no evidence for a luteovirid helper virus was found for PpVQ [7], a double-stranded RNA (dsRNA) virus, known as papaya meleira virus (PMeV), was found as the helper virus for PMeV-2 [4]. Interestingly, a similar umbra-like virus was reported in commercial papaya plants from Mexico and referred to as papaya meleira virus -Mexican variant (PMeV-Mx) [5]. As for genome features, citrus yellow vein associated virus (CYVaV), an unpublished umbra-like sequence record (GenBank Accession NC_040311), along with three recently described virus sequences namely opuntia umbra-like virus (OpUV), Ethiopian maize associated virus (EMaV) and sugarcane umbra-like virus (ScUV) possess shorter 2.6 -2.9 kb genomes arranged similar to typical umbravirus genomes [6,8].…”

Section: Introductionmentioning

confidence: 99%

“…Umbraviruses have 4 -4.2 kb genomes containing four open reading frames (ORFs) but lack a capsid protein (CP) gene, which makes them dependent on a 'helper' virus, usually from the Luteoviridae, for genome encapsidation and plant-to-plant transmission [1,2]. In recent years, several viral RNA sequences discovered from different hosts have been regarded as "umbralike" viruses based on sequence homology to the RNA-dependent-RNA-polymerase (RdRp) [3][4][5][6]. However, biological and genomic differences warrant a further examination to determine whether these viruses should be included within the Umbravirus or belong to a distinct genus of umbra-related viruses.…”

Section: Introductionmentioning

confidence: 99%

An Umbra-related Virus Found in Vasconcellea X Heilbornii (Caricaceae)

Cornejo-Franco

Flores

Mollov

et al. 2021

Preprint

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The complete sequence of a new viral RNA from babaco (Vasconcellea x heilbornii) was determined. The genome consisted of 4,584 nucleotides organized in two non-overlapping open reading frames (ORFs 1 and 2), a 9-nt-long noncoding region (NCR) at the 5’ terminus and a 1,843 -nt-long NCR at the 3’ terminus. Sequence comparisons of ORF 2 revealed homology to the RNA-dependent-RNA-polymerase (RdRp) of several umbra- and umbra-related viruses. Phylogenetic analysis of the RdRp placed the new virus in a well-supported and cohesive clade that includes umbra-like viruses reported from papaya, citrus, opuntia, maize and sugarcane hosts. This clade shares a most recent ancestor with the umbraviruses but has different genomic features. The creation of a new genus, within the Tombusviridae, is proposed for the classification of these novel viruses.

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Early diagnosis of a Mexican variant of Papaya meleira virus (PMeV-Mx) by RT-PCR

Cited by 17 publications

References 11 publications

Network Analysis of the Papaya Orchard Virome from Two Agroecological Regions of Chiapas, Mexico

Network Analysis of the Papaya Orchard Virome from Two Agroecological Regions of Chiapas, Mexico

The dsRNA Virus Papaya Meleira Virus and an ssRNA Virus Are Associated with Papaya Sticky Disease

An Umbra-related Virus Found in Vasconcellea X Heilbornii (Caricaceae)

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