Early diagnosis of amanitin exposure (amatoxicosis) in a dog with a point‐of‐care diagnostic test

Kaae, Jennifer A.; Bever, Candace S.; Poppenga, Robert H.

doi:10.1111/vec.13235

Cited by 3 publications

(2 citation statements)

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“…5 Amatoxins effectively inhibit eukaryotic RNA polymerase II, hindering mRNA transcription and protein synthesis and ultimately leading to cellular necrosis. A single toxic mushroom may contain over 20 mg of amatoxins, 6 and the average concentration of α-amanitin in mushrooms ranges from approximately 0.5 to 2 mg/g. 7 The estimated oral median lethal dose (LD50) for α-amanitin is 0.1 mg/kg in humans.…”

Section: ■ Introductionmentioning

confidence: 99%

Ultrasensitive Aptasensor for α-Amatoxin Detection Based on the DNA Tetrahedral Nanostructure Triggering Rolling Circle Amplification and Signal Amplification

Tian,

Sun,

et al. 2024

J. Agric. Food Chem.

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Poisonous mushrooms containing α-amatoxin can be lethal, making it imperative to develop a rapid and sensitive detection method for α-amatoxin. Utilizing the DNA tetrahedral structure as its foundation, the aptamer allows controlled density and orientation. Consequently, we designed aptamer tetrahedral functionalized magnetic beads that specifically target α-amanitin to release complementary DNA (C-DNA) strands. These strands were then employed as primers to initiate rolling circle amplification (RCA) with fluorescent dyes. The combination of SYBR Green I detection probes facilitated the amplification of the detection signal, enhancing the detection sensitivity of the aptasensor. The calculated detection limit was determined to be 3 ng/mL, a magnitude lower than that of other aptasensors by 2 orders of magnitude. The aptasensor integrates the advantages of high sensitivity and specificity, offering a simple and reliable rapid detection method for α-amanitin analysis.

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Section: ■ Introductionmentioning

confidence: 99%

Ultrasensitive Aptasensor for α-Amatoxin Detection Based on the DNA Tetrahedral Nanostructure Triggering Rolling Circle Amplification and Signal Amplification

Tian,

Sun,

et al. 2024

J. Agric. Food Chem.

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“…In one retrospective study, 13/59 dogs (26%) with confirmed alpha-amanitin exposure survived discharge, and none of these patients were documented to have developed adrenal insufficiency after intoxication at follow-up appointments [ 3 ]. Smaller studies have documented longer follow-up periods without evidence of adrenal disease [ 8 , 9 ]. A clearer understanding of post-intoxication outcomes would help veterinarians prepare pet owners to understand the implications and long-term consequences of amanitin intoxication.…”

Section: Introductionmentioning

confidence: 99%

Hypoadrenocorticism in a Dog Following Recovery from Alpha-Amanitin Intoxication

Cohen

Moeller

Dear

2023

Veterinary Sciences

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A 10-year-old, female spayed Labrador Retriever was referred for acute hepatopathy and urinary retention. Blood work from the initial presentation (day 0) revealed a severe, mixed hepatopathy. Over the course of the patient’s hospitalization, the patient developed liver insufficiency. Urine was submitted for toxicological screening and revealed detection of a trace concentration of alpha-amanitin. The patient was treated supportively for alpha-amanitin intoxication and was discharged from the hospital on day 8, with most biochemical parameters being markedly improved. The patient was persistently hyporexic at the time of discharge. On day 15, at a recheck appointment, the patient had lost 2.4 kg and liver enzymology revealed improved values. On day 24, the patient was presented for anorexia and vomiting and had lost another 2.3 kg. Blood work and endocrinological testing at that time were consistent with hypoadrenocorticism. The patient was started on glucocorticoids and mineralocorticoids. At day 106, the patient was doing well clinically while receiving monthly mineralocorticoids and daily glucocorticoids. This case report is the first to describe the chronological association between alpha-amanitin-induced liver dysfunction and the subsequent development of adrenal insufficiency in a dog.

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Highly Sensitive Fluorescent Aptasensor for Detecting Α-Amatoxin Based on Rolling Circle Amplification Triggered by Aptamer-Tetrahedral

Tian

et al. 2023

Preprint

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Early diagnosis of amanitin exposure (amatoxicosis) in a dog with a point‐of‐care diagnostic test

Abstract: Objective: To describe the rapid diagnosis, treatment, and clinical course of a dog that ingested an amanitin-containing mushroom.

Cited by 3 publications

References 21 publications

Ultrasensitive Aptasensor for α-Amatoxin Detection Based on the DNA Tetrahedral Nanostructure Triggering Rolling Circle Amplification and Signal Amplification

Ultrasensitive Aptasensor for α-Amatoxin Detection Based on the DNA Tetrahedral Nanostructure Triggering Rolling Circle Amplification and Signal Amplification

Hypoadrenocorticism in a Dog Following Recovery from Alpha-Amanitin Intoxication

Highly Sensitive Fluorescent Aptasensor for Detecting Α-Amatoxin Based on Rolling Circle Amplification Triggered by Aptamer-Tetrahedral

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