Early diagnosis of heterotopic ossification with a NIR fluorescent probe by targeting type II collagen

Abstract

Heterotopic ossification (HO) is a devastating sequela in which the pathologic extracellular matrix of cartilage and bone forms abnormally in soft tissues following traumatic injuries or orthopaedic surgeries. Early treatment...

NLRP3‐Dependent Crosstalk between Pyroptotic Macrophage and Senescent Cell Orchestrates Trauma‐Induced Heterotopic Ossification During Aberrant Wound Healing

“…The murine burn/tenotomy model was generated as the trauma‐induced heterotopic ossification model according to previously reported methods. [ 60 , 61 ] Only male mice were employed for modeling since there might be differential immune responses between male and female mice and thus create data variability. Briefly, male C57BL/6 mice aged 7–8 weeks were anesthetized with 1% pentobarbital sodium.…”

NIR-triggered photodynamic therapy of traumatic heterotopic ossification with a type II collagen-targeted photosensitizer

“…Inhibiting or minimizing HO formation by targeting the early phase of cartilage formation has been shown to be effective [ 15 ]. Previously, we assessed the potential of a near-infrared probe, WL-808, for early diagnosis of HO by targeting ectopic cartilage lesions [ 31 ]. In this study, we aimed to evaluate the therapeutic potential of WL-808 for the innovative HO-PDT approach.…”

“…In our prior work, we developed a near-infrared (NIR) probe called WL-808 for early HO diagnosing by targeting type II collagen (Col2a1) in the HO cartilage lesions, which allows detecting HO formation as early as one one-week post-surgery through NIR imaging [ 31 ]. The probe consists of a Col2a1-binding peptide (WYRGRL) and a cyanine dye (IR-808), of which IR-808 has demonstrated excellent photodynamic properties for treating tumors [ 32 ].…”