Early effect of laser irradiation in signaling pathways of diabetic rat submandibular salivary glands

Fukuoka, Cíntia Yuki; Vicari, Hugo Passos; Sipert, Carla Renata; Bhawal, Ujjal K.; Abiko, Yoshimitsu; Arana-Chavez, Victor E.; Simões, Alyne

doi:10.1371/journal.pone.0236727

Cited by 2 publications

(1 citation statement)

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“…Accumulating evidence suggests that salivary glands are very susceptible to stress conditions, such as radiation injury, hyperglycemia, and continuous exposure to xenobiotics in vivo (12)(13)(14). However, to the best of our knowledge, no studies have investigated whether, and to what extent, the MCD diet induces changes in the salivary glands.…”

Section: Morphological Changes In Major Salivary Glands In Micementioning

confidence: 99%

Morphological Changes in Major Salivary Glands in Mice Treated With a Choline and Methionine Deficient Diet

THOMAZ

Ribeiro

Santos

et al. 2022

In Vivo

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Background/Aim: It has been shown that the methionine-choline deficient (MCD) diet induces hepatocarcinogenesis, but not in extrahepatic organs, such as the testis, and pancreas, although may increase chemicalinduced carcinogenesis in the colon, mammary gland, esophagus, and pancreas. Accumulating evidence suggests that salivary glands are very susceptible to stress conditions, such as radiation, hyperglycemia, and exposure to xenobiotics in vivo. This study aimed to analyze the histological changes on the major salivary glands (parotid, submandibular, and sublingual) after MCD diet administration. Materials and Methods: Male Swiss mice were submitted to ad libitum access to the control or MCD diet for 28 days. The rebound group received the MCD diet for 24 days and the control diet for 10 days. Using the AxioImager A2 microscope, the hematoxylin-eosin (HE) stained specimens (4 mm) were evaluated for tissue degeneration, nuclear hyperchromatism and atrophy. Results: In the parotid gland from the MCD group, tissue degeneration, pyknosis, apoptosis and atrophy were observed, which remained in the rebound group, associated with hyperchromatism. In the submandibular gland from both MCD and rebound groups, severe tissue disorganization was associated with cell pleomorphism, hyperchromatic cells, apoptosis, increased eosinophilia, and inflammatory infiltrate. Finally, in the sublingual gland, there were no histological alterations in the experimental groups compared to the control. Conclusion: MCD can induce pre-neoplastic changes in the mouse parotid and submandibular glands, which are not reversed by a change in the diet.

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Section: Morphological Changes In Major Salivary Glands In Micementioning

confidence: 99%