Early effects of gamma rays and protons on human melanoma cell viability and morphology

Todorović, D. M.; Petrović, Ivana; Todorović, Miloš; Cuttone, G.; Ristić-Fira, Aleksandra

doi:10.1111/j.1365-2818.2008.02151.x

Cited by 9 publications

(6 citation statements)

References 10 publications

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“…Experiments involving populations of proliferative melanoma cells are performed using non-irradiated SK-MEL-28 cells [ 10 ]. Experiments where melanoma cell proliferation is suppressed are performed using irradiated, but otherwise identical SK-MEL-28 cells [ 11 , 12 ]. The melanoma cells are gamma-irradiated to inhibit mitosis.…”

Section: Resultsmentioning

confidence: 99%

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Three-dimensional experiments and individual based simulations show that cell proliferation drives melanoma nest formation in human skin tissue

et al. 2018

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BackgroundMelanoma can be diagnosed by identifying nests of cells on the skin surface. Understanding the processes that drive nest formation is important as these processes could be potential targets for new cancer drugs. Cell proliferation and cell migration are two potential mechanisms that could conceivably drive melanoma nest formation. However, it is unclear which one of these two putative mechanisms plays a dominant role in driving nest formation.ResultsWe use a suite of three-dimensional (3D) experiments in human skin tissue and a parallel series of 3D individual-based simulations to explore whether cell migration or cell proliferation plays a dominant role in nest formation. In the experiments we measure nest formation in populations of irradiated (non-proliferative) and non-irradiated (proliferative) melanoma cells, cultured together with primary keratinocyte and fibroblast cells on a 3D experimental human skin model. Results show that nest size depends on initial cell number and is driven primarily by cell proliferation rather than cell migration.ConclusionsNest size depends on cell number, and is driven primarily by cell proliferation rather than cell migration. All experimental results are consistent with simulation data from a 3D individual based model (IBM) of cell migration and cell proliferation.Electronic supplementary materialThe online version of this article (10.1186/s12918-018-0559-9) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

“…The melanoma cells are gamma-irradiated to inhibit mitosis. It is possible that irradiation may have other impacts on cellular behaviour and could also influence DNA functioning [ 12 , 13 ]. We perform a series of live assays to show that irradiation does not affect the adherence or morphology of melanoma cells.…”

Section: Resultsmentioning

confidence: 99%

Three-dimensional experiments and individual based simulations show that cell proliferation drives melanoma nest formation in human skin tissue

et al. 2018

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“…Melanoma metastatic cell line HTB140 response to proton beam irradiation was intensively studied [ 22 , 23 ]. It was shown that in comparison to gamma-rays, proton beam radiation induced more apoptotic cells, for doses ranging from 8 to 24 Gy [ 23 ]. In our study, we have focused on sublethal doses of radiation 1–5 Gy and observed slightly lower metabolic activity and decreased number of highly proliferative clones ( S2 Fig and Fig 1 ).…”

Section: Discussionmentioning

confidence: 99%

Proton beam irradiation inhibits the migration of melanoma cells

et al. 2017

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PurposeIn recent years experimental data have indicated that low-energy proton beam radiation might induce a difference in cellular migration in comparison to photons. We therefore set out to compare the effect of proton beam irradiation and X-rays on the survival and long-term migratory properties of two cell lines: uveal melanoma Mel270 and skin melanoma BLM.Materials and methodsCells treated with either proton beam or X-rays were analyzed for their survival using clonogenic assay and MTT test. Long-term migratory properties were assessed with time-lapse monitoring of individual cell movements, wound test and transpore migration, while the expression of the related proteins was measured with western blot.ResultsExposure to proton beam and X-rays led to similar survival but the quality of the cell colonies was markedly different. More paraclones with a low proliferative activity and fewer highly-proliferative holoclones were found after proton beam irradiation in comparison to X-rays. At 20 or 40 days post-irradiation, migratory capacity was decreased more by proton beam than by X-rays. The beta-1-integrin level was decreased in Mel270 cells after both types of radiation, while vimentin, a marker of EMT, was increased in BLM cells only.ConclusionsWe conclude that proton beam irradiation induced long-term inhibition of cellular motility, as well as changes in the level of beta-1 integrin and vimentin. If confirmed, the change in the quality, but not in the number of colonies after proton beam irradiation might favor tumor growth inhibition after fractionated proton therapy.

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“…This cell cycle delay provides time for DNA to repair and allows recovery of cells in contrast to apoptosis induction at higher dose rates [5]. The measurement of cell viability plays a fundamental role in predicting of tissue/cell response to different types of radiation [18,19]. While these data are preliminary, our laboratory is further exploring the differences in the modes of cell death in the in vitro HPBL model following irradiation with protons and photons.…”

Section: Resultsmentioning

confidence: 99%

Effects of 60 MeV Protons and 250 kV X-Rays on Cell Viability

et al. 2016

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Particle radiotherapy such as the one using proton beams, provides a successful treatment approach in many cancer types. However, the cellular and molecular mechanisms by which proton irradiation induces cell death, particularly in a human peripheral blood lymphocyte model has not been examined in detail. Comparative studies of the biological effects, such as cell death, of particle therapy versus conventional X-rays treatment are of utmost importance. Here, we compared the viability of human peripheral blood lymphocyte following in vitro irradiation with protons (therapeutic 60 MeV proton beam) and photon beam (250 kV, X-rays), by applying separate doses within the range of 0.3-4.0 Gy. Cell viability was assessed 1 and 4 h after irradiation with protons and X-rays by the FITC-Annexin V labelling procedure (Apoptotic & Necrotic & Healthy Cells Quantification Kit, Biotium). Results showed that irradiation with both radiation types reduced the number of viable cells in a dose-dependent manner, as assessed as a function of the duration of post-irradiation time. Protons proved more fatal to the cells treated than X-ray photons. This demonstrates a difference in cell viability after irradiation with protons and photons in a human peripheral blood lymphocyte model.

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Early effects of gamma rays and protons on human melanoma cell viability and morphology

Cited by 9 publications

References 10 publications

Three-dimensional experiments and individual based simulations show that cell proliferation drives melanoma nest formation in human skin tissue

Three-dimensional experiments and individual based simulations show that cell proliferation drives melanoma nest formation in human skin tissue

Proton beam irradiation inhibits the migration of melanoma cells

Effects of 60 MeV Protons and 250 kV X-Rays on Cell Viability

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