Early engraftment kinetics of two units cord blood transplantation

Kang, Hyoung Jin; Kho, S. H.; Jang, Mi Kyoung; Lee, S. H.; Shin, Hee Young; Ahn, Hyo Seop

doi:10.1038/sj.bmt.1705423

Cited by 36 publications

(35 citation statements)

References 9 publications

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“…Other supportive care was provided according to the guidelines for SCT of our center. 10,11 The patients were divided into three groups. Patients with ferritin level above 1000 ng/ml at the time of HSCT were classified into the first group (F41000).…”

Section: Methodsmentioning

confidence: 99%

Effect of iron overload and iron-chelating therapy on allogeneic hematopoietic SCT in children

Lee

Kang

Kim

et al. 2009

Bone Marrow Transplant

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Section: Methodsmentioning

confidence: 99%

Effect of iron overload and iron-chelating therapy on allogeneic hematopoietic SCT in children

Lee

Kang

Kim

et al. 2009

Bone Marrow Transplant

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“…For CBT, hematopoietic chimerism was analyzed more frequently at 1, 2 and 3 weeks with peripheral blood and 1, 3, 6 months and 1 year with BM aspirate by the same method. 14 The method was based on the quantitative amplification of informative polymorphic STR regions in the recipient and donor, using the AmpFlSTR profiler PCR amplification kit (Applied Biosystems, Foster City, CA, USA), as per the manufacturer's instructions.…”

Section: Chimerism Analysismentioning

confidence: 99%

Peri-engraftment syndrome in allogeneic hematopoietic SCT

Hong

Kang

Kim

et al. 2012

Bone Marrow Transplant

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Engraftment syndrome (ES) and pre-engraftment syndrome (pre-ES) are both inflammatory conditions that occur after hematopoietic SCT (HSCT) and are characterized by non-infectious fever and skin rash. Although the pathogenesis is not fully understood, both syndromes are similar, and could be defined as a new clinical syndrome, named as peri-engraftment syndrome (peri-ES). We retrospectively analyzed the clinical records in 176 pediatric patients, following allogeneic HSCT. We utilized the definition of ES by Spitzer as the diagnostic criteria, excluding 'within 96 h of engraftment' criteria. Thirty cases developed peri-ES with a cumulative incidence of 17.0%. High cumulative incidence (50%) was seen in patients who underwent a double-unit cord blood transplantation (DUCBT; Po0.01). Clinical findings of peri-ES are similar, regardless of the onset day, and encephalopathy was the most severe complication. In the DUCBT cohort, the use of TBI and early complete chimerism (pday 21) were identified as risk factors that predispose the development of peri-ES. We determined that both, ES and pre-ES, might have similar causes, which could be included in peri-ES. Particularly, it occurred more in DUCBT patients, which means that not only neutrophil engraftment but also immune reactions within the two units might contribute to peri-ES.

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“…1,[4][5][6][7][8][9] These results are difficult to interpret because insufficient details are provided in any study regarding the ad hoc computational procedures followed, the accuracy of the results or whether the measurements were assessed for reliability. It is uncertain whether any previous study analyzed loci with shared alleles, despite their common occurrence in DD HSCTs.…”

Section: Introductionmentioning

confidence: 78%

“…[1][2][3][4][5][6][7][8][9][10][11][12][13] However, the complex chimeric states associated with these transplants engender novel quantitative problems for the monitoring laboratory. The most challenging issue in analyzing multi-donor chimeras is the almost universal occurrence of shared alleles, that is, STR peaks (bands) with identical alleles from more than one DNA source.…”

Section: Discussionmentioning

confidence: 99%

“…It is most commonly exemplified in DD cord blood transplants, and combined cord blood and haploidentical transplants. [1][2][3][4][5][6][7][8][9][10][11][12][13] However, similar complex chimeric states can occur after sequential singledonor HSCTs and in the setting of feto-maternal chimerism (CHM) followed by HSCT. [14][15][16][17][18][19] Despite their advantages, such clinical achievements also engender new challenges for post-transplantation monitoring, particularly in regard to the molecular evaluation of chimeric status.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative monitoring of multi-donor chimerism: a systematic, validated framework for routine analysis

Kristt

Gesundheit

Stein

et al. 2009

Bone Marrow Transplant

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Despite therapeutic advantages, double-donor (DD) HSCTs present technical problems for molecular chimerism (CHM) monitoring. These DD chimeras contain three matched DNAs, so that the genomes of donor(s) and recipient often share the same alleles. In the STR assay, shared recipient/donor alleles are common and have identical physico-chemical properties. As a consequence of the latter, they co-migrate in the same band ('shared peak'), which prevents measuring each allele separately. Without individual allelic measurements, the direct calculation of the chimeric recipient/donor DNA ratio is precluded. This is the first study to document and systematically examine these problems. Its goal was to provide a validated framework for accurate, routine monitoring based on a stepwise analytic paradigm for approximating percent CHM (%CHM) from shared STR-alleles. Analysis of STR-DNA from DD loci showed that at least four of six alleles were typically shared. Despite such extensive allelic sharing, we show how simple arithmetic procedures can be applied for standardized calculation of %CHM based on peak measurements. Criteria for selecting loci suitable for such analysis are provided. Validation of the computational results required analyzing 18 'informative' loci with pre-established reference values for %CHM. In all cases, the results for %CHM, calculated from peak measurements, were ± 5% of the reference value. The conclusions of the study are as follows: (1) Multi-donor chimeras, with shared alleles, can be accurately and simply analyzed within the usual limits of STR measurement error; (2) by examining these various facets of DD CHM analysis, this novel study has provided a basis for standardized, routine quantitative monitoring using the STR/VNTR assay.

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Early engraftment kinetics of two units cord blood transplantation

Cited by 36 publications

References 9 publications

Effect of iron overload and iron-chelating therapy on allogeneic hematopoietic SCT in children

Effect of iron overload and iron-chelating therapy on allogeneic hematopoietic SCT in children

Peri-engraftment syndrome in allogeneic hematopoietic SCT

Quantitative monitoring of multi-donor chimerism: a systematic, validated framework for routine analysis

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