Early events in dendritic cell maturation induced by LPS

Granucci, Francesca; Ferrero, Elisabetta; Foti, Maria; Aggujaro, Diego; Vettoretto, Katuscia; Ricciardi‐Castagnoli, Paola

doi:10.1016/s1286-4579(99)00209-9

Cited by 123 publications

(93 citation statements)

References 26 publications

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“…To explore the potential involvement of TLR in the maturation of DC, we used a model system in which DC are driven to mature by established agonists of TLR (29,30). Fig.…”

Section: Heparan Sulfate and Tlr Agonists Induce DC Maturationmentioning

confidence: 99%

Receptor-Mediated Monitoring of Tissue Well-Being Via Detection of Soluble Heparan Sulfate by Toll-Like Receptor 4

Johnson¹,

Brunn²,

Kodaira

et al. 2002

The Journal of Immunology

595

370

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Perturbations to the well-being of tissues in plants and invertebrates generate fragments of endogenous molecules that are recognized by innate immune receptors. Vertebrates have homologous receptors on specialized cells such as dendritic cells, but whether these receptors respond to fragments of endogenous molecules is not known. We tested the idea that Toll-like receptors on dendritic cells might recognize polysaccharide fragments of heparan sulfate proteoglycan. Dendritic cells were found to mature in response to heparan sulfate as measured by costimulatory protein expression, morphology, and T lymphocyte stimulation, but this maturation was absent when Toll-like receptor 4 was mutated or inhibited. These findings suggest that Toll-like receptors in vertebrates may monitor tissue well-being by recognizing fragments of endogenous macromolecules.

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“…To explore the potential involvement of TLR in the maturation of DC, we used a model system in which DC are driven to mature by established agonists of TLR (29,30). Fig.…”

Section: Heparan Sulfate and Tlr Agonists Induce DC Maturationmentioning

confidence: 99%

Receptor-Mediated Monitoring of Tissue Well-Being Via Detection of Soluble Heparan Sulfate by Toll-Like Receptor 4

Johnson¹,

Brunn²,

Kodaira

et al. 2002

The Journal of Immunology

595

370

View full text Add to dashboard Cite

show abstract

“…Although DCs generally enhance the expression of CCR7 and acquire migratory ability to lymphoid tissues by various maturation stimuli, an increase in CCR7 mRNA levels and enhancement of migratory activity toward CCL21 were not observed in LPS/DCs, contrary to our expectation. Granucci et al 19 reported that DCs stimulated with LPS significantly decreased their intrinsic migratory ability and increased the antigen uptake function at 1-2 h post-stimulation. This phenomenon probably represents the in vivo stage during which DCs remain at an inflammation site caused by the components of invading bacteria, such as LPS, and capture antigens by full endocytosis.…”

Section: Discussionmentioning

confidence: 99%

Augmentation of the migratory ability of DC-based vaccine into regional lymph nodes by efficient CCR7 gene transduction

et al. 2004

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Although dendritic cell (DC)-based immunotherapy is considered a promising approach for cancer treatment, a large quantity of DC vaccine is required for effective sensitization/ activation of immune cells because of the poor migratory ability of administered DCs into regional lymphoid tissue. In this study, we created a DC vaccine sufficiently transduced with CC chemokine receptor-7 gene (CCR7/DCs) by applying RGD fiber-mutant adenovirus vector (AdRGD), and investigated its immunological characteristics and therapeutic efficacy. CCR7/DCs acquired strong chemotactic activity for CC chemokine ligand-21 (CCL21) and exhibited an immunophenotype similar to mature DCs but not immature DCs with regard to major histocompatibility complex/costimulatory molecule-expression levels and allogenic T cell proliferation-stimulating ability, while maintaining inherent endocytotic activity. Importantly, CCR7/DCs injected intradermally into mice could accumulate in draining lymph nodes about 5.5-fold more efficiently than control AdRGD-applied DCs. Reflecting these properties of CCR7/DCs, DC vaccine genetically engineered to simultaneously express endogenous antigen and CCR7 could elicit more effective antigenspecific immune response in vivo using a lower dosage than DC vaccine transduced with antigen alone. Therefore, the application of CCR7/DCs having positive migratory ability to lymphoid tissues may contribute to reduction of efforts and costs associated with DC vaccine preparation by considerably reducing the DC vaccine dosage needed to achieve effective treatment by DC-based immunotherapy.

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“…However, SOCS1 could effectively inhibit maturation induced by the vector itself. LPS is a well-characterized inducer of DC maturation (25). To investigate whether SOCS1 gene expression could maintain iDC in an immature state in the presence of exogenous maturation stimuli, DC-SOCS1, DC-VNG, or iDCs were stimulated with LPS (1 g/ml) for 24 h. Following LPS stimulation, iDCs and DC-VNG expressed high levels of MHC class II, CD40, and CD80, whereas DC-SOCS1 retained low levels of these maturation markers (Figure 2A).…”

Section: Dc-socs1 Resistant To Maturation Expresses a High Level Of Imentioning

confidence: 99%

Dendritic Cells Transduced with SOCS1 Gene Exhibit Regulatory DC Properties and Prolong Allograft Survival

et al. 2009

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SOCS1 is a key regulator of cytokine signaling and is important for maintaining balance in the immune system. It is thought to participate in negative feedback loops in cytokine signaling and may be an important signal for the regulation of dendritic cell (DC) maturation. However, it remains unclear whether DCs transduced with SOCS1 exhibit characteristics of regulatory DCs and induce allogeneic T-cell hyporesponsiveness. In this study, we constructed adenoviral vector coding SOCS1 (

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Early events in dendritic cell maturation induced by LPS

Cited by 123 publications

References 26 publications

Receptor-Mediated Monitoring of Tissue Well-Being Via Detection of Soluble Heparan Sulfate by Toll-Like Receptor 4

Receptor-Mediated Monitoring of Tissue Well-Being Via Detection of Soluble Heparan Sulfate by Toll-Like Receptor 4

Augmentation of the migratory ability of DC-based vaccine into regional lymph nodes by efficient CCR7 gene transduction

Dendritic Cells Transduced with SOCS1 Gene Exhibit Regulatory DC Properties and Prolong Allograft Survival

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