Early evidence of a regulated response to hypoxaemia in sheep that preserves the brain cortex

Gorman, D; Lin, HY; Williams, Christopher E.

doi:10.1016/j.neulet.2005.10.075

Cited by 8 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Protein kinase C gamma (PRKCG, also known as PKC-gamma, PKCγ, and SCA14) is located on chromosome 19 and encodes the γ isoform of the PKC family [ 51 , 52 ]. PKC is a family of protein serine/threonine kinases composed of multiple isoforms that play essential roles in cell mitosis and proliferation, apoptosis, and platelet activation [ 53 ]. It has been shown that PRKCG is associated with brain disorders caused by hypoxia.…”

Section: Discussionmentioning

confidence: 99%

AZU1 (HBP/CAP37) and PRKCG (PKC-gamma) may be candidate genes affecting the severity of acute mountain sickness

Shen

2023

BMC Med Genomics

View full text Add to dashboard Cite

Background Acute Mountain Sickness (AMS) is one of the diseases that predispose to sudden ascent to high altitudes above 2500 m. Among the many studies on the occurrence and development of AMS, there are few studies on the severity of AMS. Some unidentified phenotypes or genes that determine the severity of AMS may be vital to elucidating the mechanisms of AMS. This study aims to explore the underlying genes or phenotypes associated with AMS severity and to provide evidence for a better understanding of the mechanisms of AMS. Methods GSE103927 dataset was downloaded from the Gene Expression Omnibus database, and a total of 19 subjects were enrolled in the study. Subjects were divided into a moderate to severe AMS (MS-AMS, 9 subjects) group and a no or mild AMS (NM-AMS, 10 subjects) group based on the Lake Louise score (LLS). Various bioinformatics analyses were used to compare the differences between the two groups. Another dataset, Real-time quantitative PCR (RT-qPCR), and another grouping method were used to validate the analysis results. Result No statistically significant differences in phenotypic and clinical data existed between the MS-AMS and NM-AMS groups. Eight differential expression genes are associated with LLS, and their biological functions are related regulating of the apoptotic process and programmed cell death. The ROC curves showed that AZU1 and PRKCG had a better predictive performance for MS-AMS. AZU1 and PRKCG were significantly associated with the severity of AMS. The expression of AZU1 and PRKCG were significantly higher in the MS-AMS group compared to the NM-AMS group. The hypoxic environment promotes the expression of AZU1 and PRKCG. The results of these analyses were validated by an alternative grouping method and RT-qPCR results. AZU1 and PRKCG were enriched in the Neutrophil extracellular trap formation pathway, suggesting the importance of this pathway in influencing the severity of AMS. Conclusion AZU1 and PRKCG may be key genes influencing the severity of acute mountain sickness, and can be used as good diagnostic or predictive indicators of the severity of AMS. Our study provides a new perspective to explore the molecular mechanism of AMS.

show abstract

Section: Discussionmentioning

confidence: 99%

AZU1 (HBP/CAP37) and PRKCG (PKC-gamma) may be candidate genes affecting the severity of acute mountain sickness

Shen

2023

BMC Med Genomics

View full text Add to dashboard Cite

show abstract

“…Protein kinase C gamma (PRKCG, also known as PKC-gamma, PKCγ, and SCA14) is located on chromosome 19 and encodes the γ isoform of the PKC family [52,53]. PKC is a family of protein serine/threonine kinases composed of multiple isoforms that play essential roles in cell mitosis and proliferation, apoptosis, and platelet activation [54]. As a pressure receptor, PRKCG is expressed mainly in brain and eye tissues [55].…”

Section: Discussionmentioning

confidence: 99%

AZU1 (HBP/CAP37) and PRKCG (PKC-gamma) may be candidate genes affecting the severity of Acute Mountain Sickness.

Shen

2022

Preprint

View full text Add to dashboard Cite

Background Acute Mountain Sickness (AMS) is one of the diseases that predispose to sudden ascent to high altitudes above 2500 meters. Among the many studies on the occurrence and development of AMS, there are few studies on the severity of AMS. Some unidentified phenotypes or genes that determine the severity of AMS may be vital to elucidating the mechanisms of AMS. This study aimed to explore essential candidate genes and phenotypes for moderate-to-severe AMS to provide evidence for a better understanding of the mechanisms of AMS. Methods Nineteen subjects from GSE103927 dataset (Downloaded from the Gene Expression Omnibus database) were classified as having moderate to severe AMS (MS-AMS) and mild or no AMS (NM-AMS) based on the Lake Louise score (LLS). Various bioinformatics analyses, such as weighted gene co-expression network analysis (WGCNA), differentially expressed genes screening, and ROC curves were used to compare the differences between the two groups. Another dataset in the GEO database and Real-time quantitative PCR (RT-qPCR) was used to validate the analysis results. Result No statistically significant differences in phenotypic and clinical data existed between the MS-AMS and NM-AMS groups. WGCNA revealed 530 genes associated with LLS. Three hundred sixty-eight differentially expressed genes between the two groups. Eight differential genes are associated with LLS, and their biological functions are related regulating of the apoptotic process and programmed cell death. The ROC curves showed that FAM86B3P, AZU1, and PRKCG had a better predictive performance for MS-AMS. The transcript levels of AZU1 and PRKCG were significantly higher in the MS-AMS group compared to the NM-AMS group, a result validated by another dataset. RT-qPCR showed that the transcript levels of AZU1 and PRKCG were elevated in BEAS-2B cells after hypobaric hypoxia treatment. AZU1 and PRKCG were enriched in the Neutrophil extracellular trap formation pathway, suggesting the importance of this pathway in influencing the severity of AMS. Conclusion AZU1 and PRKCG were finally identified as the candidate genes affecting AMS severity. AZU1 and PRKCG may affect the severity of AMS by influencing lung endothelial cell permeability through the Neutrophil extracellular trap formation pathway. Our study provides a new perspective to explore the molecular mechanism of AMS, and inhibiting or targeting these genes may help high altitude acclimation and reduce the occurrence of AMS or moderate to severe AMS.

show abstract

“… 6 Some researchers believed that it was caused by oxygen deficit. 7 Other previous studies indicated that DEACMP was associated with the inflammatory reaction in blood vessels, and immune damage. 8 , 9 Chen et al 10 reported that the excitatory amino acid might play an important role in the occurrence of DEACMP.…”

Section: Introductionmentioning

confidence: 91%

Combined application of dexamethasone and hyperbaric oxygen therapy yields better efficacy for patients with delayed encephalopathy after acute carbon monoxide poisoning

Xiang¹,

Hou²,

Wang³

et al. 2017

DDDT

View full text Add to dashboard Cite

BackgroundDelayed encephalopathy after acute carbon monoxide (CO) poisoning (DEACMP) commonly occurs after recovering from acute CO poisoning. This study was performed to assess the efficacy of the combined application of dexamethasone and hyperbaric oxygen (HBO) therapy in patients with DEACMP.Patients and methodsA total of 120 patients with DEACMP were recruited and randomly assigned into the experimental group (receiving dexamethasone 5 mg/day or 10 mg/day plus HBO therapy) and control group (HBO therapy as monotherapy). Meanwhile, the conventional treatments were provided for all the patients. We used the Mini-Mental State Examination (MMSE) scale to assess the cognitive function, the National Institutes of Health Stroke Scale (NIHSS) to assess the neurological function and the remission rate (RR) to assess the clinical efficacy. Myelin basic protein (MBP) in the cerebrospinal fluid (CSF) was also measured.ResultsAfter 4 weeks of treatment, compared to the control group, the experimental group had a significantly higher remission rate (P=0.032), a significantly higher average MMSE score (P=0.037) and a significantly lower average NIHSS score (P=0.002). Meanwhile, there was a trend toward better improvement with dexamethasone 10 mg/day, and the level of MBP in the CSF of patients was significantly lower in the experimental group than in the control group (P<0.0001). The addition of dexamethasone did not significantly increase the incidence of adverse events.ConclusionThese results indicate that the combined application of dexamethasone and HBO therapy could yield better efficacy for patients with DEACMP and should be viewed as a potential new therapy.

show abstract

Early evidence of a regulated response to hypoxaemia in sheep that preserves the brain cortex

Cited by 8 publications

References 29 publications

AZU1 (HBP/CAP37) and PRKCG (PKC-gamma) may be candidate genes affecting the severity of acute mountain sickness

AZU1 (HBP/CAP37) and PRKCG (PKC-gamma) may be candidate genes affecting the severity of acute mountain sickness

AZU1 (HBP/CAP37) and PRKCG (PKC-gamma) may be candidate genes affecting the severity of Acute Mountain Sickness.

Combined application of dexamethasone and hyperbaric oxygen therapy yields better efficacy for patients with delayed encephalopathy after acute carbon monoxide poisoning

Contact Info

Product

Resources

About