Early feeding of rainbow trout (Oncorhynchus mykiss) with methionine deficient diet over a two-week period: consequences for liver mitochondria in juveniles

Abstract

Methionine is a key factor in modulating the cellular availability of the main biological methyl donor S-adenosylmethionine (SAM), which is required for all biological methylation reactions including DNA and histone methylation. As such, it represents a potential critical factor in nutritional programming. Here, we investigated whether early methionine restriction at first feeding could have long-term programmed metabolic consequences in rainbow trout. For this purpose, trout fry were fed with either a control diet (C) or a methionine-deficient diet (MD) for 2 weeks from the first exogenous feeding. Next, fish were subjected to a 5 month growth trial with a standard diet followed by a 2 week challenge (with the MD or C diet) to test the programming effect of the early methionine restriction. The results showed that, whatever the dietary treatment of fry, the 2 week challenge with the MD diet led to a general mitochondrial defect associated with an increase in endoplasmic reticulum stress, mitophagy and apoptosis, highlighting the existence of complex cross-talk between these different functions. Moreover, for the first time, we also observed that fish fed the MD diet at the first meal later exhibited an increase in several critical factors of mitophagy, hinting that the early nutritional stimulus with methionine deficiency resulted in long-term programming of this cell function. Together, these data extend our understanding of the role of dietary methionine and emphasize the potential for this amino acid in the application of new feeding strategies, such as nutritional programming, to optimize the nutrition and health of farmed fish.

Co‐feeding dry and live feed in first‐feeding gilthead seabream: Effects on functional development of the digestive system, larvae and postlarvae performance

“…In agreement to present results, co-feeding regimes from mouth opening have been reported to enhance growth and survival of Senegalese sole (Cañavate & Fernández-Díaz, 1999;Engrola, Figueira, et al, 2009) and loach (Wang et al, 2009) after weaning. Present results also corroborate the scientific findings that indicate that changes in environment or nutrition during the critical developmental stages may influence the subsequent postlarvae and juvenile performance (Koedijk et al, 2010;López-et al, 2003;Perera & Yúfera, 2016;Séité et al, 2019;Vagner et al, 2007).…”

The myxozoans Myxobolus cerebralis and Tetracapsuloides bryosalmonae modulate rainbow trout immune responses: quantitative shotgun proteomics at the portals of entry after single and co-infections

“…PARPs are involved in chromatin regulation, transcription, RNA biology, and DNA repair. PARP, as a prominent marker of apoptosis was upregulated in rainbow trout, revealing apoptosis induction under endoplasmic reticulum stress ( Séité et al., 2019 ). PARP acts on both host and viral proteins through binding to deltex E3 ubiquitin ligase 3L (DTX3L) to mediate efficient immune responses to pathogens ( Gupte et al., 2017 ).…”

Modulation of Energy Metabolism and Epigenetic Landscape in Rainbow Trout Fry by a Parental Low Protein/High Carbohydrate Diet

“…In teleost fish, although only few studies have been conducted, the concept of programming has also been demonstrated in different species [15], and a particular focus has been given to aquaculture species [16]. Several studies have focused on different stimuli given during the early feeding phase and their long term consequences [17][18][19][20][21]. The consequences of stimuli applied earlier in development i.e., during gametogenesis, have also been tested.…”