Early growth reduction in Vicia faba L. under alkali salt stress is mainly caused by excess bicarbonate and related to citrate and malate over accumulation

Sagervanshi, Amit; Akhtar, Naeem; Kaiser, Hartmut; Pitann, Britta; Mühling, Karl H.

doi:10.1016/j.envexpbot.2021.104636

Cited by 17 publications

(8 citation statements)

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“…However, Ca 2+ is antagonistic to Na + , and the excessive uptake of Na + leads to Ca 2+ deficiency in cotton. Some scholars believe that the decrease of Ca 2+ and Fe 2+ in roots is related to the accumulation of organic acids in roots, the organic acids formed insoluble complexes with Fe 2+ and Ca 2+ in the roots which impaired nutrient uptake ( Sagervanshi et al, 2021 ). Our studies have shown that salt and alkali stresses limit the uptake of Fe 2+ and Ca 2+ by cotton, especially under alkali stress.…”

Section: Discussionmentioning

confidence: 99%

Comparative Ionomics and Metabolic Responses and Adaptive Strategies of Cotton to Salt and Alkali Stress

Guo

Tao

et al. 2022

Front. Plant Sci.

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Soil salinization and alkalization severely inhibit agriculture. However, the response mechanisms of cotton to salt stress or alkali stress are unclear. Ionomics and metabolomics were used to investigate salt and alkali stresses in cotton roots and leaves. Compared with the control, salt-treated and alkali-treated cotton plants showed 51.8 and 53.0% decreases in biomass, respectively. Under salt stress, the concentration of N decreased in roots but increased in leaves, and the concentrations of P and K increased in roots but decreased in leaves. Salt stress inhibited Ca, B, N, and Fe uptake and Mg, K, P, S, and Cu transport, but promoted Mo, Mn, Zn, Mg, K, P, S, and Cu uptake and Mo, Mn, Zn, B, N, and Fe transport. Under alkali stress, the concentrations of N and P in roots and leaves decreased, while the concentrations of K in roots and leaves increased. Alkali stress inhibited P, Ca, S, N, Fe, and Zn uptake and N, P, Mg and B transport, but promoted K, Mn, Cu, Mo, Mg, and B uptake and K, Mn, Cu, Mo, Fe, and Zn transport. Under salt stress in the leaves, 93 metabolites increased, mainly organic acids, amino acids, and sugars, increased in abundance, while 6 decreased. In the roots, 72 metabolites increased, mainly amino acids, organic acids, and sugars, while 18 decreased. Under alkali stress, in the leaves, 96 metabolites increased, including organic acids, amino acids, and sugars, 83 metabolites decreased, including organic acids, amino acids, and sugars; In the roots, 108 metabolites increased, including organic acids, amino acids, and sugars. 83 metabolites decreased, including organic acids and amino acids. Under salt stress, cotton adapts to osmotic stress through the accumulation of organic acids, amino acids and sugars, while under alkali stress, osmoregulation was achieved via inorganic ion accumulation. Under salt stress, significant metabolic pathways in the leaves and roots were associated with amino acid and organic acid metabolism, sugar metabolism was mainly used as a source of energy, while under alkali stress, the pathways in the leaves were related to amino acid and linoleic acid metabolism, β-Oxidation, TCA cycle, and glycolysis were enhanced to provide the energy needed for life activities. Enhancing organic acid accumulation and metabolism in the roots is the key response mechanism of cotton to alkalinity.

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Section: Discussionmentioning

confidence: 99%

Comparative Ionomics and Metabolic Responses and Adaptive Strategies of Cotton to Salt and Alkali Stress

Guo

Tao

et al. 2022

Front. Plant Sci.

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“…The uptake of bicarbonate can cause important metabolic disturbances due to both alteration of cell pH homoeostasis and dark fixation of inorganic carbon. Bicarbonate can be quickly incorporated into organic acids, mainly malate, causing inhibition of mineral nutrient transport from roots to shoot, reduction of root growth, and oxidative stress in sensitive calcifuges, but not in tolerant calcicole species (Lee & Woolhouse, 1969; Sagervanshi et al, 2021). Due to these multiple direct and indirect injuries, sensitive species exhibit a complex syndrome of stress symptoms, including morpho‐anatomical changes in roots, disturbance of water relations, signs of iron and/or zinc deficiency, reduction of total photosynthetic pigments and photosynthetic activity; accumulation of osmo‐protectants, soluble sugars, and organic acids; and activation of the biosynthesis of antioxidant enzymes (Ahmad et al, 2014; Sarkar et al, 2022; Zou et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, after several decades of effort still a proper model is missing to understand the adaptive mechanism to carbonate stress and the tolerance mechanism at the molecular‐genetic level. Early experiments revealed that organic acid accumulation in response to bicarbonate occurred both in sensitive and tolerant species and it was speculated that differential compartmentation of organic acids may play a role in the tolerance (Sagervanshi et al, 2021). In fact, several anion transporters, and gene families such as ALMT, NRT/POT and SLAHs were related to bicarbonate response in G. max (Duan et al, 2018; Waters et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Combined genomics to discover genes associated with tolerance to soil carbonate

Busoms

Pérez‐Martín

Terés

et al. 2023

Plant Cell & Environment

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Carbonate‐rich soils limit plant performance and crop production. Previously, local adaptation to carbonated soils was detected in wild Arabidopsis thaliana accessions, allowing the selection of two demes with contrasting phenotypes: A1 (carbonate tolerant, c+) and T6 (carbonate sensitive, c−). Here, A1(c+) and T6(c−) seedlings were grown hydroponically under control (pH 5.9) and bicarbonate conditions (10 mM NaHCO3, pH 8.3) to obtain ionomic profiles and conduct transcriptomic analysis. In parallel, A1(c+) and T6(c−) parental lines and their progeny were cultivated on carbonated soil to evaluate fitness and segregation patterns. To understand the genetic architecture beyond the contrasted phenotypes, a bulk segregant analysis sequencing (BSA‐Seq) was performed. Transcriptomics revealed 208 root and 2503 leaf differentially expressed genes in A1(c+) versus T6(c−) comparison under bicarbonate stress, mainly involved in iron, nitrogen and carbon metabolism, hormones and glycosylates biosynthesis. Based on A1(c+) and T6(c−) genome contrasts and BSA‐Seq analysis, 69 genes were associated with carbonate tolerance. Comparative analysis of genomics and transcriptomics discovered a final set of 18 genes involved in bicarbonate stress responses that may have relevant roles in soil carbonate tolerance.

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“…Castor seeds regulate the osmotic balance and ROS level by promoting amino acid metabolism and the tricarboxylic acid cycle under alkali stress (Han et al 2022). Vicia faba adapts to early alkaline salt stress by accumulating organic acids such as citric acid and malic acid (Sagervanshi et al 2021).…”

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confidence: 99%

Transcriptome and Metabolome Integrated Analysis Reveals the Mechanism of Cinnamomum bodinieri Root Response to Alkali Stress

Han¹,

Zhang²,

Li³

et al. 2023

Plant Mol Biol Rep

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Cinnamomun bodinieri's normal growth and development are hampered by alkali stress, impeding its production and application of Cinnamomum bodinieri. The root organs being in direct contact with the cultivation environment, are sensitive to environmental stress. The present study revealed the differentially expressed genes and differentially metabolized products of Cinnamomum bodinieri root under alkali stress employing transcriptome and metabonomic analysis. The ndings revealed that 690 differentially expressed genes and 269 metabolites were signi cantly different among HT6 and HCK6. Similarly, 1000 differentially expressed genes and 360 metabolites with signi cant differences were identi ed in HT48.vs.HCK48 combination. The combined analysis of transcriptome and metabolome identi ed 9 metabolic pathways at 6h and 48h after alkali treatment, including the biosynthesis pathway of tropane, piperidine and pyridine alkaloids, pyrimidine metabolic pathway, phenylalanine metabolic pathway, isoquinoline alkaloid biosynthesis pathway, glycolysis/gluconeogenesis pathway, avonoid biosynthesis pathway, fatty acid biosynthesis pathway, carbon xation pathway in photosynthetic organisms, the metabolic pathway of amino sugar and nucleotide sugar. Therefore, the strategy of Cinnamomum bodinieri to cope with alkali stress may be to increase osmotic regulation and antioxidant activity by accumulating alkaloids, avonoids secondary metabolites, and N-acetyl-L-phenylalanine, ensure the stability of cell structure and function through the accumulation of lauric acid and palmitic acid, provide energy for plants to withstand alkali stress by accelerating the glycolysis process, and improve plants' resistance to biological and abiotic stress by inducing the activity of chitinase, The accumulation of oxaloacetic acid and other organic acids alleviates alkali stress environment. This study provides support for the analysis of the pathways and regulatory networks of Cinnamomum bodinieri in response to alkali stress.

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Early growth reduction in Vicia faba L. under alkali salt stress is mainly caused by excess bicarbonate and related to citrate and malate over accumulation

Cited by 17 publications

References 70 publications

Comparative Ionomics and Metabolic Responses and Adaptive Strategies of Cotton to Salt and Alkali Stress

Comparative Ionomics and Metabolic Responses and Adaptive Strategies of Cotton to Salt and Alkali Stress

Combined genomics to discover genes associated with tolerance to soil carbonate

Transcriptome and Metabolome Integrated Analysis Reveals the Mechanism of Cinnamomum bodinieri Root Response to Alkali Stress

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