Early impairment of skeletal muscle endothelial glycocalyx barrier properties in diet-induced obesity in mice

Eskens, Bart; Leurgans, Thomas; Vink, Hans; VanTeeffelen, Jurgen W. G. E.

doi:10.1002/phy2.194

Cited by 26 publications

(15 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have overcome this limitation by combining fluorescence images with differential interference contrast image or by subtracting the endothelial cell edge with a red blood cell trace [23,24]. Until now, the typical method for quantifying glycocalyx thickness involved calculating the full width at half maximum [25,26].…”

Section: Discussionmentioning

confidence: 99%

In vivo Imaging of the Cerebral Endothelial Glycocalyx in Mice

Yoon

Lee²,

Jeong³

2017

J Vasc Res

View full text Add to dashboard Cite

Background/Aims: Endothelial glycocalyx refers to the proteoglycan or glycoprotein layer of vessel walls and has critical physiological functions. Cerebral glycocalyx may have additional functions considering the blood-brain barrier and other features. However, the assessment of it has only been performed ex vivo, which includes processes presumably damaging the glycocalyx layer. Here we visualize and characterize the cerebral endothelial glycocalyx in vivo. Methods: We visualized and quantified the cerebral endothelial glycocalyx in vivo under a 2-photon microscope by tagging glycocalyx and vessel lumen with wheat germ agglutinin lectin and dextran, respectively. The radial intensity was analyzed to measure the thickness of the cerebral endothelial glycocalyx in each vessel type. Results: Cerebral arteries and capillaries have an intact endothelial glycocalyx, but veins and venules do not. The thickness of the glycocalyx layer in pial arteries, penetrating arteries, and capillaries was different; however, it was not correlated with the vessel diameter within each vessel type. Conclusion: We characterized the distribution of the cerebral endothelial glycocalyx in vivo. Compared to the results from ex vivo studies, the layer is thicker, indicating that the layer may be damaged in ex vivo systems. We also observed an inhomogeneous cerebral endothelial glycocalyx distribution that might reflect the functional heterogeneity of the vessel type.

show abstract

Section: Discussionmentioning

confidence: 99%

In vivo Imaging of the Cerebral Endothelial Glycocalyx in Mice

Yoon

Lee²,

Jeong³

2017

J Vasc Res

View full text Add to dashboard Cite

show abstract

“…This process seems to be impaired by insulin resistance in type 2 diabetes and obesity ( 83 ). Mechanistically, damage to the endothelial glycocalyx barrier in skeletal muscle is believed to be an early event, as shown in mice fed an HFD ( 84 ). A recent study showed that endothelial insulin resistance also plays a pivotal role in the regulation of glucose uptake by the skeletal muscle ( 83 ).…”

Section: Endothelial Regulation Of Obesity-associated Insulin Resistamentioning

confidence: 99%

Vascular Endothelial Regulation of Obesity-Associated Insulin Resistance

Qian

2017

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

Obesity is a worldwide epidemic that predisposes individuals to metabolic complications, such as type 2 diabetes mellitus and non-alcoholic fatty liver disease, all of which are related to an imbalance between food intake and energy expenditure. Identification of the pathogenic molecular mechanisms and effective therapeutic approaches are urgently needed. A well-accepted paradigm is that crosstalk between organs/tissues contributes to diseases. Endothelial dysfunction characterizes metabolic disorders and the related vascular complications. Over the past two decades, overwhelming studies have focused on mechanisms that lead to endothelial dysfunction. New investigations, however, have begun to appreciate the opposite direction of the crosstalk: endothelial regulation of metabolism, although the underlying mechanisms remain to be elucidated. This review summarizes the evidence that supports the concept of endothelial regulation of obesity and the associated insulin resistance in fat, liver, and skeletal muscles, the classic targets of insulin. Outstanding questions and future research directions are highlighted. Identification of the mechanisms of vascular endothelial regulation of metabolism may offer strategies for prevention and treatment of obesity and the related metabolic complications.

show abstract

“…All data presented herein were obtained from refs. [44,54,55,70,72,74,75,78,88,95,96,98,102–111,116,119–121,124–130,132,134–137,140–144,147–156,158,159,161,163–171,173–176...…”

Section: Diet Composition and Exposure Timementioning

confidence: 99%

Modeling Diet‐Induced Metabolic Syndrome in Rodents

Leonardi

Gosmann

Zimmer

2020

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Standardized animal models represent one of the most valuable tools available to understand the mechanism underlying the metabolic syndrome (MetS) and to seek for new therapeutic strategies. However, there is considerable variability in the studies conducted with this essential purpose. This review presents an updated discussion of the most recent studies using diverse experimental conditions to induce MetS in rodents with unbalanced diets, discusses the key findings in metabolic outcomes, and critically evaluates what we have been learned from them and how to advance in the field. The study includes scientific reports sourced from the Web of Science and PubMed databases, published between January 2013 and June 2020, which used hypercaloric diets to induce metabolic disorders, and address the impact of the diet on metabolic parameters. The collected data are used as support to discuss variables such as sex, species, and age of the animals, the most favorable type of diet, and the ideal diet length to generate metabolic changes. The experimental characteristics propose herein improve the performance of a preclinical model that resembles the human MetS and will guide researchers to investigate new therapeutic alternatives with confidence and higher translational validity.

show abstract

Early impairment of skeletal muscle endothelial glycocalyx barrier properties in diet-induced obesity in mice

Cited by 26 publications

References 56 publications

In vivo Imaging of the Cerebral Endothelial Glycocalyx in Mice

In vivo Imaging of the Cerebral Endothelial Glycocalyx in Mice

Vascular Endothelial Regulation of Obesity-Associated Insulin Resistance

Modeling Diet‐Induced Metabolic Syndrome in Rodents

Contact Info

Product

Resources

About