Early inflammatory response in soft tissues induced by thin calcium phosphates

Rydén, Louise; Molnár, Dávid; Esposito, Marco; Johansson, Anna; Suska, Felicia; Palmquist, Anders; Thomsen, Peter

doi:10.1002/jbm.a.34571

Cited by 20 publications

(10 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present findings are in partial agreement with previous results in a rat soft-tissue model, using identical amorphous and crystalline HA-coated titanium implants [15]. In the latter study, a lower inflammatory response was demonstrated for both types of HA surface and a smaller number of cells adhered to amorphous HA compared with titanium, as determined by the amount of DNA in the implant-adherent cells [15].…”

Section: Discussionsupporting

confidence: 93%

“…In the latter study, a lower inflammatory response was demonstrated for both types of HA surface and a smaller number of cells adhered to amorphous HA compared with titanium, as determined by the amount of DNA in the implant-adherent cells [15]. Furthermore, relatively lower cytotoxicity, as measured by LD analysis, and lower MCP-1 secretion were detected in response to amorphous HA and, to a lesser extent, at the crystalline HA, when both were compared with titanium [15]. The reason for the discrepancy between the present in vitro findings and the soft-tissue findings cannot currently be determined.…”

Section: Discussionmentioning

confidence: 99%

“…Titanium implants with thin HA coatings elicit a less intense inflammatory reaction when compared with a titanium surface in a soft-tissue model [15]. However, although some trends were observed, the study did not reveal any major difference in cytokine secretions when comparing the thin crystalline and amorphous HA coatings.…”

Section: Introductionmentioning

confidence: 89%

See 2 more Smart Citations

Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces

Rydén

Omar

Johansson

et al. 2016

J Mater Sci: Mater Med

View full text Add to dashboard Cite

It has been suggested that surface modification with a thin hydroxyapatite (HA) coating enhances the osseointegration of titanium implants. However, there is insufficient information about the biological processes involved in the HA-induced response. This study aimed to investigate the inflammatory cell response to titanium implants with either amorphous or crystalline thin HA. Human mononuclear cells were cultured on titanium discs with a machined surface or with a thin, 0.1 μm, amorphous or crystalline HA coating. Cells were cultured for 24 and 96 h, with and without lipopolysaccharide (LPS) stimulation. The surfaces were characterized with respect to chemistry, phase composition, wettability and topography. Biological analyses included the percentage of implant-adherent cells and the secretion of pro-inflammatory cytokine (TNF-α) and growth factors (BMP-2 and TGF-β1). Crystalline HA revealed a smooth surface, whereas the amorphous HA displayed a porous structure, at nano-scale, and a hydrophobic surface. Higher TNF-α secretion and a higher ratio of adherent cells were demonstrated for the amorphous HA compared with the crystalline HA. TGF-β1 secretion was detected in all groups, but without any difference. No BMP-2 secretion was detected in any of the groups. The addition of LPS resulted in a significant increase in TNF-α in all groups, whereas TGF-β1 was not affected. Taken together, the results show that thin HA coatings with similar micro-roughness but a different phase composition, nano-scale roughness and wettability are associated with different monocyte responses. In the absence of strong inflammatory stimuli, crystalline hydroxyapatite elicits a lower inflammatory response compared with amorphous hydroxyapatite.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces

Rydén

Omar

Johansson

et al. 2016

J Mater Sci: Mater Med

View full text Add to dashboard Cite

show abstract

“…Although in certain in vivo experiments inflammatory reactions were observed after implantation of calcium orthophosphate bioceramics [655,656,657,658,659,660,661,662], the general conclusion on using calcium orthophosphates with Ca/P ionic ratio within 1.0–1.7 is that all types of implants (bioceramics of various porosities and structures, powders or granules) are not only nontoxic but also induce neither inflammatory nor foreign-body reactions [128,663,664]. The biological response to implanted calcium orthophosphates follows a similar cascade observed in fracture healing.…”

Section: Biological Properties and In Vivo Behaviormentioning

confidence: 99%

Calcium Orthophosphate-Based Bioceramics

Dorozhkin¹

2013

Materials

231

153

View full text Add to dashboard Cite

Various types of grafts have been traditionally used to restore damaged bones. In the late 1960s, a strong interest was raised in studying ceramics as potential bone grafts due to their biomechanical properties. A bit later, such synthetic biomaterials were called bioceramics. In principle, bioceramics can be prepared from diverse materials but this review is limited to calcium orthophosphate-based formulations only, which possess the specific advantages due to the chemical similarity to mammalian bones and teeth. During the past 40 years, there have been a number of important achievements in this field. Namely, after the initial development of bioceramics that was just tolerated in the physiological environment, an emphasis was shifted towards the formulations able to form direct chemical bonds with the adjacent bones. Afterwards, by the structural and compositional controls, it became possible to choose whether the calcium orthophosphate-based implants remain biologically stable once incorporated into the skeletal structure or whether they were resorbed over time. At the turn of the millennium, a new concept of regenerative bioceramics was developed and such formulations became an integrated part of the tissue engineering approach. Now calcium orthophosphate scaffolds are designed to induce bone formation and vascularization. These scaffolds are often porous and harbor different biomolecules and/or cells. Therefore, current biomedical applications of calcium orthophosphate bioceramics include bone augmentations, artificial bone grafts, maxillofacial reconstruction, spinal fusion, periodontal disease repairs and bone fillers after tumor surgery. Perspective future applications comprise drug delivery and tissue engineering purposes because calcium orthophosphates appear to be promising carriers of growth factors, bioactive peptides and various types of cells.

show abstract

“…Soft tissue metal implants, including titanium based structures, posses a proclivity to commence inflammatory reactions resulting in tissue fibrosis [37]. Although mechanisms of inflammation are being fully delineated, it is believed that the innate immune response, particularly the M1 macrophage component, is responsible for eventual fibrosis.…”

Section: Discussionmentioning

confidence: 99%

The promotion of functional urinary bladder regeneration using anti-inflammatory nanofibers

Bury¹,

Fuller²,

Meisner³

et al. 2014

Biomaterials

View full text Add to dashboard Cite

Current attempts at tissue regeneration utilizing synthetic and decellularized biologic-based materials have typically been met in part by innate immune responses in the form of a robust inflammatory reaction at the site of implantation or grafting. This can ultimately lead to tissue fibrosis with direct negative impact on tissue growth, development, and function. In order to temper the innate inflammatory response, anti-inflammatory signals were incorporated through display on self-assembling peptide nanofibers to promote tissue healing and subsequent graft compliance throughout the regenerative process. Utilizing an established urinary bladder augmentation model, the highly pro-inflammatory biologic scaffold (decellularized small intestinal submucosa) was treated with anti-inflammatory peptide amphiphiles (AIF-PAs) or control peptide amphiphiles and used for augmentation. Significant regenerative advantages of the AIF-PAs were observed including potent angiogenic responses, limited tissue collagen accumulation, and the modulation of macrophage and neutrophil responses in regenerated bladder tissue. Upon further characterization, a reduction in the levels of M2 macrophages was observed, but not in M1 macrophages in control groups, while treatment groups exhibited decreased levels of M1 macrophages and stabilized levels of M2 macrophages. Pro-inflammatory cytokine production was decreased while anti-inflammatory cytokines were up-regulated in treatment groups. This resulted in far fewer incidences of tissue granuloma and bladder stone formation. Finally, functional urinary bladder testing revealed greater bladder compliance and similar capacities in groups treated with AIF-PAs. Data demonstrate that AIF-PAs can alleviate galvanic innate immune responses and provide a highly conducive regenerative milieu that may be applicable in a variety of clinical settings.

show abstract

Early inflammatory response in soft tissues induced by thin calcium phosphates

Cited by 20 publications

References 46 publications

Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces

Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces

Calcium Orthophosphate-Based Bioceramics

The promotion of functional urinary bladder regeneration using anti-inflammatory nanofibers

Contact Info

Product

Resources

About