Early Introduction of Plant Polysaccharides Drives the Establishment of Rabbit Gut Bacterial Ecosystems and the Acquisition of Microbial Functions

Paës, Charlotte; Bebin, Karine; Duperray, Joël; Gohier, Charly; Guené-Grand, Emeline; Rebours, G.; Barilly, Céline; Gabinaud, Beatrice; Cauquil, Laurent; Castinel, Adrien; Pascal, Géraldine; Darbot, Vincent; Aymard, Patrick; Debrusse, Anne-Marie; Beaumont, Martín; Combes, Sylvie

doi:10.1128/msystems.00243-22

Cited by 8 publications

(6 citation statements)

References 74 publications

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“…Beaumont et al [ 29 ] found that the relative concentrations of acetate, butyrate, propionate, glucose, and glutamate in the intestines of juvenile rabbits increased with age and the relative abundance of Firmicutes increased with age. Charlotte et al [ 30 ] demonstrated that very early ingestion of solid food in infant rabbits, although in small quantities, induced changes in gut microbiota colonization and activity, with an acceleration of the ecological species succession and increased production of short-chain fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Study on Changes in Gut Microbiota and Microbiability in Rabbits at Different Developmental Stages

Fu,

Ma,

Xia

et al. 2024

Animals

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This study used feces from 0-day-old (36 rabbits), 10-day-old (119 rabbits), and 60-day-old (119 rabbits) offspring rabbits and their corresponding female rabbits (36 rabbits) as experimental materials. Using 16s rRNA sequencing, the study analyzed the types and changes of gut microbiota in rabbits at different growth and development stages, as well as the correlation between gut microbiota composition and the weight of 60-day-old rabbits. All experimental rabbits were placed in the same rabbit shed. Juvenile rabbits were fed solid feed at 18 days of age and weaned at 35 days of age. In addition to identifying the dominant bacterial phyla of gut microbiota in rabbits at different age stages, it was found that the abundance of Clostridium tertium and Clostridium paraputrificum in all suckling rabbits (10-day-old) was significantly higher than that in rabbits fed with whole feed (60-day-old) (p < 0.05), while the abundance of Gram-negative bacterium cTPY13 was significantly lower (p < 0.05). In addition, Fast Expected Maximum Microbial Source Tracing (FEAST) analysis showed that the contribution of female rabbits’ gut microbiota to the colonization of offspring rabbits’ gut microbiota was significantly higher than that of unrelated rabbits’ gut microbiota (p < 0.05). The contribution of female rabbits’ gut microbiota to the colonization of gut microbiota in 0-day-old rabbits was significantly higher than that to the colonization of gut microbiota in the 10- and 60-day-old rabbits (p < 0.05). Finally, the correlation between gut microbiota composition and body weight of 60-day-old rabbits was analyzed based on a mixed linear model, and six ASVs significantly affecting body weight were screened. The above results provide important theoretical and practical guidance for maintaining gut health, improving growth and development performance, and feeding formulation in rabbits.

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Section: Discussionmentioning

confidence: 99%

Study on Changes in Gut Microbiota and Microbiability in Rabbits at Different Developmental Stages

Fu,

Ma,

Xia

et al. 2024

Animals

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“…In comparison, nonrumination herbivores (e.g., horse, donkey, and rabbit) showed significantly different stomach microbiota compositions during the development, as enzyme-based chemical and fermentative digestions were found in the stomach of nonruminants and ruminants, respectively. Actually, the cecum and gut seem to be more important in the studies of nonruminants, for example, rabbits (Paës et al 2022). Previous studies have shown that Firmicutes and Bacteroidetes were the dominant microbes in the stomach or rumen of adult herbivores (Supplemental Table S49; Jin et al 2018;Guo et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Single-cell transcriptome and metagenome profiling reveals the genetic basis of rumen functions and convergent developmental patterns in ruminants

Deng,

Liu,

Wei

et al. 2023

Genome Res.

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The rumen undergoes developmental changes during maturation. To characterize this understudied dynamic process, we profiled single-cell transcriptomes of about 308,000 cells from the rumen tissues of sheep and goats at 17 time points. We built comprehensive transcriptome and metagenome atlases from early embryonic to rumination stages, and recapitulated histomorphometric and transcriptional features of the rumen, revealing key transitional signatures associated with the development of ruminal cells, microbiota, and core transcriptional regulatory networks. In addition, we identified and validated potential cross-talk between host cells and microbiomes and revealed their roles in modulating the spatiotemporal expression of key genes in ruminal cells. Cross-species analyses revealed convergent developmental patterns of cellular heterogeneity, gene expression, and cell–cell and microbiome–cell interactions. Finally, we uncovered how the interactions can act upon the symbiotic rumen system to modify the processes of fermentation, fiber digestion, and immune defense. These results significantly enhance understanding of the genetic basis of the unique roles of rumen.

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“…1 and 7-day old piglets, vs those that did have access, i.e. 28, 36 and 180-day old pigs, assuming that the luminal environment would differ [17,18]. Interestingly, the percentage of organoids to spheroids in the primary culture was greater for organoids originating from younger pigs that did not had access to solid feed than older ones that did (Fig 3D ) and this ratio was maintained with increasing number of passages (Fig 3F and G).…”

Section: Donor Age Influenced Organoid Growthmentioning

confidence: 99%

Initial pig developmental stage influences intestinal organoid growth but not phenotype

Duchesne,

Randuineau,

Normand

et al. 2024

Preprint

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Intestinal organoids are promising tools in the context of animal experiment reduction. Yet, a thorough characterization of the impact of the origin of intestinal stem cells (ISC) on organoid phenotype is needed to routinely use this cellular model. Our objective was to evaluate the effect of ISC donor age on the growth, morphology and cellular composition of intestinal organoids derived from pig, a valuable model of Humans. Organoids were derived from jejunal and colonic ISC obtained from 1, 7, 28, 36 and 180-day old pigs and passaged three times. We first confirmed by qPCR that the expression of 18% of the >80 studied genes related to various intestinal functions differed between jejunal and colonic organoids after two passages (P<0.05). Growth and morphology of organoids depended on intestinal location (greater number and larger organoids derived from colonic than jejunal ISC, P<0.05) but also pig age. Indeed, when ISC were derived from young piglets, the ratio of organoids to spheroids was greater (P<0.05), spheroids were larger during the primary culture but smaller after two passages (P<0.05) and organoids smaller after one passage (P>0.05) compared to ISC from older pigs. Finally, no difference in cellular composition, evaluated by immunostaining of markers of the major intestinal cell types (absorptive, enteroendocrine and goblet cells) were observed between organoids originating from 7 or 180-day old pigs, while difference between intestinal site origin were noticed. In conclusion, while the age of the tissue donor affected organoid growth and morphology, it did not influence their phenotype.

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Early Introduction of Plant Polysaccharides Drives the Establishment of Rabbit Gut Bacterial Ecosystems and the Acquisition of Microbial Functions

Cited by 8 publications

References 74 publications

Study on Changes in Gut Microbiota and Microbiability in Rabbits at Different Developmental Stages

Study on Changes in Gut Microbiota and Microbiability in Rabbits at Different Developmental Stages

Single-cell transcriptome and metagenome profiling reveals the genetic basis of rumen functions and convergent developmental patterns in ruminants

Initial pig developmental stage influences intestinal organoid growth but not phenotype

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