Early massive follicle loss and apoptosis in heterotopically grafted newborn mouse ovaries

Abstract

The results of the present study indicate that transplantation rather than cryopreservation accounts for the major and early loss of primordial follicles in grafted newborn mouse ovaries.

Beneficial effect of desialylated erythropoietin administration on the frozen-thawed canine ovarian xenotransplantation

“…However, a majority of primordial follicles in frozen-thawed canine ovarian tissues reportedly disappears after transplantation ( Table 1). The main reason for the follicular loss after cryopreservation and xenografting seems to be the ischemic effect which takes place after transplantation rather than cryopreservation per se [16,29]. Several attempts have been The results are shown as the mean±SD.…”

“…The main drawback of ovarian cryopreservation followed by transplantation is that a large proportion of follicles are lost during the initial ischemia which occurs after transplantation [9][10][11][12][13][14][15][16]. Therefore, reducing the damage due to the ischemic interval between transplantation and revascularization is essential to maintaining both follicular reserve and the function of the graft.…”

Blastocyst Development After Cryopreservation and Subcutaneous Transplantation of Mouse Ovarian Tissue

“…In the study of Liu et al cryopreserved primordial follicles developed to preantral follicles by in vivo grafting but were then recovered and cultured to maturity by IVM (4). The same group also found apoptosis and impaired function in frozen and thawed granulosa cells after transplantation (24). It was suggested that granulosa cells are more active metabolically than primordial oocytes and thus were more subject to ischemic damage.…”

Human oocyte and ovarian tissue cryopreservation and its application

“…In sheep, autotransplantation of frozen-thawed ovarian cortex [85] and of hemi-ovaries [94] has resulted in pregnancies, deliveries and prolonged hormone production [7,8]. Nevertheless, in all these cases there was a reduction in the total follicular number due to ischemia, therefore ovarian function was transient [95]. Only eight of 80 human oocytes aspirated from a cryopreserved transplanted ovary were suitable for IVF and only one oocyte fertilized normally [96].…”

“…The first human pregnancy by cryopreserved ovarian cortex and transplantation was recently reported [9]. It appears that the main obstacles to successful restoration of fertility from frozenthawed ovarian cortex are adhesions and the massive ischemic damage to follicles until neovascularization develops [95]. Most follicles which survive cryopreservation undergo ischemic loss during the time required for neovascularization [97].…”