Early Osteogenic Differentiation Stimulation of Dental Pulp Stem Cells by Calcitriol and Curcumin

Abstract

Curcumin, as a natural phenolic substance, is extracted from the rhizome of Curcuma longa (turmeric), which is effective in bone healthfulness. Calcitriol is an effective hormone in regulating bone remodeling and mineral homeostasis and immune response. Mesenchymal stem cells (MSCs) are found in most dental tissues and resemble bone marrow-derived MSCs. In this work, we investigated the effect of combination and individual treatment of curcumin and calcitriol on early osteogenic differentiation of dental pulp stem cells (DPSCs). Early osteogenic differentiation was evaluated and confirmed by the gene expression level of ALP and its activity. Curcumin individually and in combination with calcitriol increased ALP activity and osteoblast-specific mRNA expression of ALP when DPSCs were cultured in an osteogenic medium. Calcitriol alone increased the enzyme more than in combination with curcumin. These findings demonstrate that curcumin can induce early osteogenic differentiation of DPSCs like calcitriol as a potent stimulant of osteogenesis.

Influence of Curcumin Nanocrystals on the Early Osteogenic Differentiation and Proliferation of Dental Pulp Stem Cells

“…Our other recent study showed that curcumin upsurges the early osteogenic differentiation of DPSCs. These outcomes were demonstrated by analyzing the activity of ALP or osteogenic gene expression levels [44]. In another study, the cytotoxicity of curcumin and the osteogenic marker expression in C3H1-T1/2 cells were tested and it was shown that curcumin improved the expression of ALP and osteocalcin (OC) genes that then differentiated osteoblasts in cells [18].…”

Identification of Di/Tripeptide(s) With Osteoblasts Proliferation Stimulation Abilities of Yak Bone Collagen by in silico Screening and Molecular Docking

“…Similarly, CF exhibited an opposite effect on the PI of osteoblasts ( Figures 3A,C,D ). ALP activity is the marker of early osteogenic differentiation ( 49 ). As shown in Figure 3E , the ALP activities of MC3T3-E1 cells treated with MGF and CF for 120 h both highly significantly ( P < 0.01) decreased, which was different from other studies ( 7 , 10 ).…”

Curcumin nanoformulations: Beneficial nanomedicine against cancer

“…Curcumin has a wide range of effects for medical application (Ghavimi et al, 2020; Samiei, Abedi, Sharifi, & Maleki Dizaj, 2021; Sharifi et al, 2019; Sharifi, Fathi, et al, 2020; Sharifi, Moghaddam, et al, 2020), which can interact with various molecular targets involved in chronic diseases (Canistro et al, 2021). Curcumin can be an alternative to chemotherapy drugs due to its anticancer properties and low side effects.…”