Early prediction of wheat quality: analysis during grain development using mass spectrometry and multivariate data analysis

Ghirardo, Andrea; Sørensen, Helle Aagaard; Petersen, Marianne; Jacobsen, Susanne; Søndergaard, Ib

doi:10.1002/rcm.1823

Cited by 24 publications

(17 citation statements)

References 23 publications

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“…The multivariate data analyses followed the established procedures to analyze MS data as described previously (Ghirardo et al, 2005(Ghirardo et al, , 2012Kreuzwieser et al, 2014;Vanzo et al, 2014;Velikova et al, 2014). The isoprene emission trait was selected as the Y variable for the OPLS analysis by setting NE = 1 and IE = 0.…”

Section: Statistical Analysesmentioning

confidence: 99%

Knocking Down of Isoprene Emission Modifies the Lipid Matrix of Thylakoid Membranes and Influences the Chloroplast Ultrastructure in Poplar

et al. 2015

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Isoprene is a small lipophilic molecule with important functions in plant protection against abiotic stresses. Here, we studied the lipid composition of thylakoid membranes and chloroplast ultrastructure in isoprene-emitting (IE) and nonisoprene-emitting (NE) poplar (Populus 3 canescens). We demonstrated that the total amount of monogalactosyldiacylglycerols, digalactosyldiacylglycerols, phospholipids, and fatty acids is reduced in chloroplasts when isoprene biosynthesis is blocked. A significantly lower amount of unsaturated fatty acids, particularly linolenic acid in NE chloroplasts, was associated with the reduced fluidity of thylakoid membranes, which in turn negatively affects photosystem II photochemical efficiency. The low photosystem II photochemical efficiency in NE plants was negatively correlated with nonphotochemical quenching and the energy-dependent component of nonphotochemical quenching. Transmission electron microscopy revealed alterations in the chloroplast ultrastructure in NE compared with IE plants. NE chloroplasts were more rounded and contained fewer grana stacks and longer stroma thylakoids, more plastoglobules, and larger associative zones between chloroplasts and mitochondria. These results strongly support the idea that in IE species, the function of this molecule is closely associated with the structural organization and functioning of plastidic membranes.

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Section: Statistical Analysesmentioning

confidence: 99%

Knocking Down of Isoprene Emission Modifies the Lipid Matrix of Thylakoid Membranes and Influences the Chloroplast Ultrastructure in Poplar

et al. 2015

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“…The protein fractions (albumins, globulins, and gliadins) were extracted from flour in triplicate according to Osborne (Ghirardo et al , 2005), and quantified according to Popov et al (1975).…”

Section: Methodsmentioning

confidence: 99%

Exploring abiotic stress on asynchronous protein metabolism in single kernels of wheat studied by NMR spectroscopy and chemometrics

Winning

Viereck

Wollenweber

et al. 2008

Journal of Experimental Botany

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Extreme climate events are being recognized as important factors in the effects on crop growth and yield. Increased climatic variability leads to more frequent extreme conditions which may result in crops being exposed to more than one extreme event within a growing season. The aim of this study was to examine the implications of different drought treatments on the protein fractions in grains of winter wheat using 1H nuclear magnetic resonance spectroscopy followed by chemometric analysis. Triticum aestivum L. cv. Vinjett was studied in a semi-field experiment and subjected to drought episodes either at terminal spikelet, during grain-filling or at both stages. Principal component trajectories of the total protein content and the protein fractions of flour as well as the 1H NMR spectra of single wheat kernels, wheat flour, and wheat methanol extracts were analysed to elucidate the metabolic development during grain-filling. The results from both the 1H NMR spectra of methanol extracts and the 1H HR-MAS NMR of single kernels showed that a single drought event during the generative stage had as strong an influence on protein metabolism as two consecutive events of drought. By contrast, a drought event at the vegetative growth stage had little effect on the parameters investigated. For the first time, 1H HR-MAS NMR spectra of grains taken during grain-filling were analysed by an advanced multiway model. In addition to the results from the chemical protein analysis and the 1H HR-MAS NMR spectra of single kernels indicating that protein metabolism is influenced by multiple drought events, the 1H NMR spectra of the methanol extracts of flour from mature grains revealed that the amount of fumaric acid is particularly sensitive to water deficits.

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“…Although, the hardness of a certain wheat variety can be genetically controlled, is not directly linked to the protein content of the grain (Simmonds, 1974;Miller et al, 1984;Huifen and Hoseney, 1991;Weegels et al 1996). Ghirardo et al (2005) used matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and multivariate data analysis for the determination of wheat quality at different stages of grain development. Wheat varieties with one of two different end-use qualities (i.e.…”

Section: Wheat Genotype and Genetic Loccimentioning

confidence: 98%

Quality and Safety Aspects of Cereals (Wheat) and Their Products

Varzakas

2015

Critical Reviews in Food Science and Nutrition

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Cereals and, most specifically, wheat are described in this chapter highlighting on their safety and quality aspects. Moreover, wheat quality aspects are adequately addressed since they are used to characterize dough properties and baking quality. Determination of dough properties is also mentioned and pasta quality is also described in this chapter. Chemometrics-multivariate analysis is one of the analyses carried out. Regarding production weighing/mixing of flours, kneading, extruded wheat flours, and sodium chloride are important processing steps/raw materials used in the manufacturing of pastry products. Staling of cereal-based products is also taken into account. Finally, safety aspects of cereal-based products are well documented with special emphasis on mycotoxins, acrylamide, and near infrared methodology.

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Early prediction of wheat quality: analysis during grain development using mass spectrometry and multivariate data analysis

Cited by 24 publications

References 23 publications

Knocking Down of Isoprene Emission Modifies the Lipid Matrix of Thylakoid Membranes and Influences the Chloroplast Ultrastructure in Poplar

Knocking Down of Isoprene Emission Modifies the Lipid Matrix of Thylakoid Membranes and Influences the Chloroplast Ultrastructure in Poplar

Exploring abiotic stress on asynchronous protein metabolism in single kernels of wheat studied by NMR spectroscopy and chemometrics

Quality and Safety Aspects of Cereals (Wheat) and Their Products

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