Early pregnancy diagnosis in buffaloes from plasma progesterone concentration

Perera, B.M.A.O.; Pathiraja, N.; Abeywardena, S. A.; Motha, M.X.J.; Abeygunawardena, H.

doi:10.1136/vr.106.5.104

Cited by 15 publications

(16 citation statements)

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“…(1983); they reported progesterone levels of 0.7–2.0 ng/ml during early pregnancy in river‐type buffalo in Sri Lanka and swamp‐type buffalo in Thailand and Malaysia. The low accuracy of the progesterone assay for determining pregnant buffalo (Sensitivity) during days 22–24 PM (66.7%) concurs with previous reports (Singh and Pathiyandy 1980; Perera et al. 1981; Gupta and Prakash 1990; Ghoneim et al.…”

Section: Discussionsupporting

confidence: 90%

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Early Pregnancy Detection of Iraqi Riverine Buffalo (Bubalus bubalis) Using the BioPRYN Enzyme‐Linked Immunosorbent Assay for PSPB and the Progesterone Assay

Abdulkareem

Al-Sharifi

Ishak

et al. 2010

Reprod Domestic Animals

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This study was undertaken to detect pregnancy in Iraqi riverine buffalo (Bubalus bubalis) using three different methods (rectal palpation, plasma progesterone concentration and detection of the presence of pregnancy-specific protein B (PSPB) with the BioPRYN(®) enzyme-linked immunosorbent assay (ELISA) test. The aim of the study was to identify the most sensitive, early and accurate method for detecting pregnancy. Twenty-two female riverine buffalo that were 6.0 ± 0.93 years old were used. Four blood samples per buffalo were taken via jugular venipuncture at days 22-24, 32-34, 42-44 and 58-61 post-mating (PM) to measure the progesterone concentration (ng/ml) and to detect the presence of plasma PSPB. The rectal palpation method was employed to evaluate all buffalo on days 42-44 and 58-61 PM. The BioPRYN(®) test differed (p<0.01) from the other tests with earlier accuracy for detecting pregnant and non-pregnant buffalo. Eighty-eight percent of pregnant and 76.9% of non-pregnant buffalo were distinguished early (days 22-24 PM) using BioPRYN(®) and plasma PSPB-ELISA level (2.09 ± 0.12 ng/ml) in relation to 66.7% and 53.9% detected using the progesterone assay at similar days (4.30 ± 0.40 ng/ml). In conclusion, these results described, for the first time, the early and accurate pregnancy detection of water riverine buffalo using BioPRYN(®) technology and provided the plasma levels of PSPB using an ELISA test. These findings will improve the reproductive and productive efficiency of Iraqi riverine buffalo by adapting the recent management and reproductive strategies in Iraq and in the world.

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Section: Discussionsupporting

confidence: 90%

“…Similarly, the current results are greater than those reported by Kamonpatana et al. (1981), Perera et al. (1981) and Jainudeen et al.…”

Section: Discussioncontrasting

confidence: 80%

Early Pregnancy Detection of Iraqi Riverine Buffalo (Bubalus bubalis) Using the BioPRYN Enzyme‐Linked Immunosorbent Assay for PSPB and the Progesterone Assay

Abdulkareem

Al-Sharifi

Ishak

et al. 2010

Reprod Domestic Animals

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“…On the other hand, the current results were higher than those recorded by Kamonpatana et al. (1981), Perera et al. (1981) and Jainudeen et al.…”

Section: Discussioncontrasting

confidence: 69%

“…Another, more classical indicator for pregnancy is the progesterone concentration in the maternal plasma. Peak progesterone levels (0.7–2.0 ng/ml) during early pregnancy were reported in blood plasma of river‐type buffaloes in Sri Lanka (Perera et al. 1981) and swamp‐type buffaloes in Thailand and Malaysia (Kamonpatana et al.…”

Section: Introductionmentioning

confidence: 99%

Pregnancy‐associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows

El-Battawy¹,

Sousa²,

Szenci³

et al. 2009

Reprod Domestic Animals

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Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg prostraglandin (PG)F(2alpha) (Lutalyse) at a 11-day interval. Blood sampling was conducted nearly twice weekly. Results indicated that plasma PAG concentrations in non-pregnant buffaloes were low (<0.20 ng/ml) during the whole experimental period (day 28 to 103), while in pregnant animals plasma PAG levels increased from day 28 (4.48 +/- 0.92 ng/ml) until day 41 (27.27 +/- 6.74 ng/ml), remaining high (20.71 +/- 9.20 ng/ml) until day 103. Progesterone levels were significantly (p < 0.0001) higher in pregnant (3.51-4.80 ng/ml) than in non-pregnant buffaloes (0.28-1.52 ng/ml). A significant difference (p < 0.0001) in plasma PAG concentrations between pregnant and non-pregnant animals starting at day 28 after breeding suggests that PAG-radioimmunoassay could be suitable for pregnancy diagnosis in buffaloes during this period. In conclusion, PAG test offers the advantages that it requires a single plasma sample for early pregnancy diagnosis as well as the accuracy of the test for the detection of pregnancy as early as day 28.

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“…Just like cattle, buffaloes too can be accurately diagnosed as nonpregnant by determination of plasma progesterone concentrations 21 days after insemination [27]. A major constraint in using progesterone assay for pregnancy diagnosis is its use only in cases where AI or breeding dates are known/recorded and not randomly in the herd.…”

Section: Pregnancy Detection Methodsmentioning

confidence: 99%

Early Pregnancy Diagnosis in Bovines: Current Status and Future Directions

Balhara

Gupta

Singh

et al. 2013

The Scientific World Journal

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An early and accurate diagnosis of reproductive dysfunctions or aberrations is crucial to better reproductive management in livestock. High reproductive efficiency is a prerequisite for high life-time production in dairy animals. Early pregnancy diagnosis is key to shorten the calving interval through early identification of open animals and their timely treatment and rebreeding so as to maintain a postpartum barren interval close to 60 days. A buffalo, the most important dairy animal in the Indian subcontinent, is known for problems related to high calving interval, late puberty, and high incidence of anestrus. Lack of reliable cow-side early pregnancy diagnosis methods further aggravates the situation. Several methods of pregnancy diagnosis are being practiced in bovine species, yet none qualifies as the ideal pregnancy diagnosis method due to the inherent limitations of sensitivity, accuracy, specificity, speed, and ease of performing the test. The advancement of molecular techniques like proteomics and their applications in animal research has given a new hope to look for pregnancy biomarker molecules in these animals. This review attempts to examine common pregnancy diagnosis methods available for dairy animals, while assessing the usefulness of the modern technologies in detecting novel pregnancy markers and designing future strategies for research in this area.

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Early pregnancy diagnosis in buffaloes from plasma progesterone concentration

Cited by 15 publications

References 0 publications

Early Pregnancy Detection of Iraqi Riverine Buffalo (Bubalus bubalis) Using the BioPRYN Enzyme‐Linked Immunosorbent Assay for PSPB and the Progesterone Assay

Early Pregnancy Detection of Iraqi Riverine Buffalo (Bubalus bubalis) Using the BioPRYN Enzyme‐Linked Immunosorbent Assay for PSPB and the Progesterone Assay

Pregnancy‐associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows

Early Pregnancy Diagnosis in Bovines: Current Status and Future Directions

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