Early prenatal diagnosis of congenital toxoplamosis using amniotic fluid samples and tissue culture

Derouin, F.; Thulliez, P.; Candolfi, E; Daffos, F.; Forestier, F.

doi:10.1007/bf01962355

Cited by 45 publications

(24 citation statements)

References 4 publications

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“…Fetal blood samples must be collected in the second term of pregnancy (when the fetal immune system is functional) by experienced obstetrical teams [29], Several biological tests must be performed to verify that fetus samples are not contaminated with maternal blood, which would interfere with diagnosis of congenital infection on the basis of parasitological and serological results [1,2,[30][31][32],…”

Section: Discussionmentioning

confidence: 99%

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Anti-P30 IgA antibodies as prenatal markers of congenital toxoplasma infection

Decoster

Darcy

Caron

et al. 1992

Clinical and Experimental Immunology

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SUMMARYThis study extends a previous study and confirms that the detection of anti-P30 IgA antibodies is very helpful in the diagnosis of acute acquired or congenital toxoplasmosis. Moreover, we demonstrate that an anti-P30 IgA response can be mounted in the fetuses infected by Toxoplasma gondii during their intra-uterine life as early as week 23 of gestation. A double-sandwich ELISA described in our previous work was used to detect anti-P30 IgA antibodies in 1378 human serum samples collected from 551 patients, including 162 fetuses whose mothers had been infected by T. gondii during pregnancy, 46 congenitally infected and 90 uninfected newborns and 253 women suspected of having been infected during pregnancy, including the mothers of fetuses and newborns previously described. Anti-P30 IgA antibodies were detected in all cases of acute toxoplasmosis but in no case of chronic toxoplasmosis: in the majority of cases, the IgA antibody titre fell below cut-off in 3-9 months. Among the 46 congenitally infected newborns, anti-P30 IgA antibodies were detected in sera of 41 infected newborns (38 at birth, two in the first months of life, one in the seventh month of life), while anti-P30 IgM antibodies were detected in only 30 cases at birth and in one case during the first month of life. Among 162 fetuses, anti-P30 IgA response was observed in five infected fetuses, but was not detected in either 152 uninfected fetuses or in five fetuses considered as infected. The absence or presence of anti-P30 IgA antibodies in the fetus is discussed in relation to the date of maternal infection and collection ofthe fetal blood. It clearly appears from our study that the combined testing of both IgM and IgA in the fetus and the newborn is essential for a more efficient diagnosis of infection.

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Section: Discussionmentioning

confidence: 99%

“…inoculation of mice [1][2][3] and on serological tests, which detect anti-toxoplasma IgG and IgM Correspondence: Dr Anne Decoster, Laboratoire St Antoine, 329 Boulevard Victor Hugo, BP 255, 59019 Lille Cedex, France.…”

Section: Introductionmentioning

confidence: 99%

Anti-P30 IgA antibodies as prenatal markers of congenital toxoplasma infection

Decoster

Darcy

Caron

et al. 1992

Clinical and Experimental Immunology

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“…The utilization of material obtained by chordocentesis for the diagnosis of infection of fetuses gave reliable results, especially if used for biological testing [21 ]. but we must bear in mind that chordocentesis can be used only after 20 weeks of gestation and that a period of 1-5 weeks is needed for biological tests.…”

Section: Discussionmentioning

confidence: 99%

Specific IgM Antibodies as Parameters of Toxoplasma Infection in Pregnancy

Sibalić

Bobić²,

Djurković–Djaković³

1993

Gynecol Obstet Invest

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The aim of this investigation was to determine the time of toxoplasma infection more precisely by using an indirect fluorescent antibody test and indirect hemagglutination test in 51 pregnant women whose Sabin-Feldman test and immunosorbent agglutination assay were positive. Analysis of the results of sera tested with the two additional serological assays showed that the infection was acquired during pregnancy in 19 cases, while the other pregnant women had become infected before conception.

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“…Furthermore, results are obtained only several weeks after inocculation. Recent studies [4,11,12] have shown human diploid fibroblast cells to be an attractive alternative to mice for isolating T. gondii because of the similar sensitivity, wider availability and higher rapidity in obtaining results.…”

Section: Isolation Of T Gondiimentioning

confidence: 99%

Laboratory diagnosis of toxoplasmosis

Loon

1989

Int Ophthalmol

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Acute infection with Toxoplasma gondii is diagnosed by (i) detection of the parasite directly in patients' specimens using histological or immunological methods, (ii) isolation of T. gondii from blood, body fluids or tissue by inoculation in laboratory mice or on tissue culture cells, or (iii) serological methods for determination of a significant, T. gondii-specific, antibody titre rise or of T. gondii specific IgM (or IgA) antibody. Because of their high sensitivity, specificity and relative ease of performance, serological methods are preferentially used for diagnosis of T. gondii infection. The diagnosis of acute, postnatally-acquired, primary toxoplasmosis is usually established by serological methods and will in general present few problems. However, diagnosis of congenital infections and their late sequelae or of reactivation of a latent infection in immunocompromised patients is often more troublesome because of the absence of a significant antibody titre rise or the lack of specific IgM antibody. In these cases a combination of various methods may be required for definitive diagnosis of T. gondii infection.

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Early prenatal diagnosis of congenital toxoplamosis using amniotic fluid samples and tissue culture

Cited by 45 publications

References 4 publications

Anti-P30 IgA antibodies as prenatal markers of congenital toxoplasma infection

Anti-P30 IgA antibodies as prenatal markers of congenital toxoplasma infection

Specific IgM Antibodies as Parameters of Toxoplasma Infection in Pregnancy

Laboratory diagnosis of toxoplasmosis

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